##gff-version 3
##sequence-region BBa_S04623 1 3420
BBa_S04623	.	engineered_region	1	1733	.	+	0	ID=BBa_K561000;Name=BBa_K561000
BBa_S04623	.	promoter	1	135	.	+	0	ID=BBa_K561001;Name=BBa_K561001;Parent=BBa_K561000
BBa_S04623	.	promoter	1	135	.	+	0	ID=annotation2146939;Name=vgb promoter;Parent=BBa_K561001
BBa_S04623	.	engineered_region	894	1733	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K561000
BBa_S04623	.	terminator	1605	1684	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_S04623	.	engineered_region	1605	1684	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04623	.	stem_loop	1616	1659	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04623	.	ribosome_entry_site	894	905	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_S04623	.	conserved_region	898	901	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04623	.	terminator	1693	1733	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_S04623	.	engineered_region	1693	1733	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04623	.	stop_codon	1726	1726	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04623	.	stem_loop	1700	1719	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04623	.	polyA_site	1720	1733	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04623	.	CDS	912	1571	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_S04623	.	engineered_tag	1572	1596	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S04623	.	tag	1532	1565	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S04623	.	CDS	915	1531	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S04623	.	engineered_region	144	885	.	+	0	ID=BBa_I15017;Name=BBa_I15017;Parent=BBa_K561000
BBa_S04623	.	CDS	163	885	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_I15017
BBa_S04623	.	ribosome_entry_site	144	156	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I15017
BBa_S04623	.	ribosome_entry_site	150	153	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04623	.	ribosome_entry_site	144	156	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04623	.	engineered_region	144	156	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04623	.	engineered_region	1742	3420	.	+	0	ID=BBa_K561002;Name=BBa_K561002
BBa_S04623	.	engineered_region	1849	2572	.	+	0	ID=BBa_K093005;Name=BBa_K093005;Parent=BBa_K561002
BBa_S04623	.	CDS	1867	2572	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K093005
BBa_S04623	.	engineered_tag	2548	2572	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04623	.	CDS	1867	2541	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04623	.	ribosome_entry_site	1849	1860	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K093005
BBa_S04623	.	conserved_region	1853	1856	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04623	.	promoter	1742	1840	.	+	0	ID=BBa_K387003;Name=BBa_K387003;Parent=BBa_K561002
BBa_S04623	.	engineered_region	2581	3420	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K561002
BBa_S04623	.	terminator	3292	3371	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_S04623	.	engineered_region	3292	3371	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04623	.	stem_loop	3303	3346	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04623	.	ribosome_entry_site	2581	2592	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_S04623	.	conserved_region	2585	2588	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04623	.	terminator	3380	3420	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_S04623	.	engineered_region	3380	3420	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04623	.	stop_codon	3413	3413	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04623	.	stem_loop	3387	3406	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04623	.	polyA_site	3407	3420	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04623	.	CDS	2599	3258	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_S04623	.	engineered_tag	3259	3283	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S04623	.	tag	3219	3252	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S04623	.	CDS	2602	3218	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
>BBa_S04623
acaggacgctggggttaaaagtatttgagttttgatgtggattaagttttaagaggcaataaagattataataagtgct
gctacaccatactgatgtatggcaaaaccataataatgaacttaaggaagaccctctactagagtcacacaggaaagta
ctagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggc
cacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccg
gcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgacca
catgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgac
ggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgact
tcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaa
gcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactac
cagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagca
aagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacga
gctgtacaagtaataatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcg
cattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcc
tacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatact
cacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaa
gtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagc
ctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgc
gtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattac
gacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggatt
agaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatag
tgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagtgaataaatagtgcccgtaatatcagggaatgaccccacataaaatgtggcataaaagatgcatactgtagtcgag
agcgcgtatgcgtgatttgattatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagag
ttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtc
cgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcccc
gcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaa
ggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacg
gtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatggg
ttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaa
gacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaa
ccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca
ctccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgtccaga
ttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaac
tcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagc
cattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataac
gctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaac
agtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcag
cgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacct
actactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttat
tcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaacta
cgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata