LOCUS BBa_K594013 1758 bp DNA linear 22-Nov-2024 DEFINITION A device that can accepts the 3--O-C6-HSL and then produces 3-O-C12-HSL ACCESSION BBa_K594013 VERSION BBa_K594013.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 RBS 1071..1085 /label=RBS-1\Strong RBS 1078..1082 /label=RBS misc_feature 1071..1085 /label=BBa_B0030 RBS 1071..1085 /label=BBa_B0030 CDS 1092..1694 /label=LasI misc_feature 1695..1727 /label=LVA misc_feature 1734..1758 /label=Help:Barcodes CDS 1092..1758 /label=BBa_C0078 misc_feature 1071..1758 /label=BBa_K081009 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag attaaagagg 1081 agaaatacta gatgatcgtt cagatcggtc gtcgtgaaga gttcgacaaa aaactgctgg 1141 gtgaaatgca caaactgcgt gctcaggttt tcaaagaacg taaaggttgg gacgtttccg 1201 ttatcgacga aatggaaatc gacggttacg acgctctgtc cccgtactac atgctgatcc 1261 aggaagacac cccggaagct caggttttcg gttgctggcg tatcttcgac accaccggtc 1321 cgtacatgct gaaaaacacc ttcccggaac tgctgcacgg taaagaagct ccgtgctccc 1381 cgcacatctg ggaactgtcc cgtttcgcta tcaactccgg tcagaaaggt tccctgggtt 1441 tctccgactg caccctggaa gctatgcgtg ctctggctcg ttactccttg cagaacgaca 1501 tccagaccct ggttaccgtt accaccgttg gtgttgaaaa aatgatgatc cgtgctggtc 1561 tggacgtttc ccgtttcggt ccgcacctga aaatcggtat cgaacgtgct gttgctctgc 1621 gtatcgaact gaacgctaaa acccagatcg ctctgtacgg tggtgttctg gttgaacagc 1681 gtctggctgt ttccgctgct aacgacgaaa actacgctct ggttgcttaa taactctgat 1741 agtgctagtg tagatctc // LOCUS BBa_S04628 1758 bp DNA linear 22-Nov-2024 DEFINITION F2622:K081009 ACCESSION BBa_S04628 VERSION BBa_S04628.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 CDS 1092..1694 /label=LasI misc_feature 1695..1727 /label=LVA misc_feature 1734..1758 /label=Help:Barcodes CDS 1092..1733 /label=BBa_C0078 RBS 1071..1085 /label=RBS-1\Strong RBS 1078..1082 /label=RBS misc_feature 1071..1085 /label=BBa_B0030 RBS 1071..1085 /label=BBa_B0030 CDS 1092..1694 /label=LasI misc_feature 1695..1727 /label=LVA misc_feature 1734..1758 /label=Help:Barcodes CDS 1092..1758 /label=BBa_C0078 misc_feature 1071..1758 /label=BBa_K081009 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag attaaagagg 1081 agaaatacta gatgatcgtt cagatcggtc gtcgtgaaga gttcgacaaa aaactgctgg 1141 gtgaaatgca caaactgcgt gctcaggttt tcaaagaacg taaaggttgg gacgtttccg 1201 ttatcgacga aatggaaatc gacggttacg acgctctgtc cccgtactac atgctgatcc 1261 aggaagacac cccggaagct caggttttcg gttgctggcg tatcttcgac accaccggtc 1321 cgtacatgct gaaaaacacc ttcccggaac tgctgcacgg taaagaagct ccgtgctccc 1381 cgcacatctg ggaactgtcc cgtttcgcta tcaactccgg tcagaaaggt tccctgggtt 1441 tctccgactg caccctggaa gctatgcgtg ctctggctcg ttactccttg cagaacgaca 1501 tccagaccct ggttaccgtt accaccgttg gtgttgaaaa aatgatgatc cgtgctggtc 1561 tggacgtttc ccgtttcggt ccgcacctga aaatcggtat cgaacgtgct gttgctctgc 1621 gtatcgaact gaacgctaaa acccagatcg ctctgtacgg tggtgttctg gttgaacagc 1681 gtctggctgt ttccgctgct aacgacgaaa actacgctct ggttgcttaa taactctgat 1741 agtgctagtg tagatctc //