BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_S04629
1
BBa_S04629
B0034:K572009
2011-10-03T11:00:00Z
2015-05-08T01:14:43Z
false
false
_9_
0
7681
9
Not in stock
false
false
Varun Chokshi
component2151863
1
BBa_K572009
component2151859
1
BBa_B0034
annotation2151859
1
BBa_B0034
range2151859
1
1
12
annotation2151863
1
BBa_K572009
range2151863
1
19
1719
BBa_K572009
1
BBa_K572009
Mouse Beta Carotene Dioxygenase
2011-10-02T11:00:00Z
2015-05-08T01:12:44Z
Source Organism : Marine gamma proteobacterium EBAC31A08.
We would like to thank Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea for sending us the plasmid (pKJ900) with dioxygenase gene.
..
false
true
_743_
0
7684
9
It's complicated
false
Forward Primer :
5???TTTACTACGAATTCGCGGCCGCTTCTAGATGGAGATAATATTTG 3???
Reverse Primer :
5???AATCGAACctgcagcggccgctactagtaTTAAAGACTTGAG 3???
Polymerase Chain reactions didn't work for a long time at various temperature conditions. Finally, increasing Magnesium Sulphate in the reaction resulted in bringing about the amplification. The following is the optimized PCR Setup :
Template (116 ng/ul) = 0.25 ul
10mM dNTP = 1.0 ul
10x Pfu Buffer (without Magnesium Sulphate) = 2.0 ul
+ Primer (10 uM) = 1.0 ul
- Primer (10 uM) = 1.0 ul
Magnesium Sulphate - final concentration of 2.5 - 3 mM
Pfu Pol = 1.25 u
Nuclease Free Water - make the volume to 20ul
false
Kousik Sundararajan
annotation2151502
1
Start Codon
range2151502
1
1
3
annotation2151504
1
Stop Codon
range2151504
1
1699
1701
annotation2151503
1
Bc_dioxygenase
range2151503
1
1
1701
BBa_K572009_sequence
1
atggagataatatttggccagaataagaaagaacagctggagccagttcaggccaaagtgacaggcagcattccagcatggctgcaggggaccctgctccgaaacgggcccgggatgcacacagtgggagagagcaagtacaaccattggtttgatggcctggcccttctccacagtttctccatcagagatggggaggtcttctacaggagcaaatacctgcagagtgacacctacatcgccaacattgaggccaacagaatcgtggtgtctgagttcggaaccatggcctacccggacccctgcaaaaacatcttttccaaagctttctcctacttgtctcacaccatccccgacttcacagacaactgtctgatcaacatcatgaaatgtggagaagacttctatgcaaccacggagaccaactacatcaggaaaatcgacccccagaccctagagactttggagaaggttgattaccggaagtatgtggcggtaaacctggctacctcgcaccctcattatgacgaggctgggaatgtccttaacatgggcacatccgtcgtggacaaagggaggacaaaatacgtgatatttaagatccctgccacagtgccagacagcaagaagaaagggaagagtcccgtgaagcacgcggaagttttctgctccatttcctcccgctcgctgctctctcccagctactaccacagctttggtgtcacggagaactatgtggtgtttctggagcagccttttaagttggatatcctcaagatggccaccgcatacatgaggggagtgagctgggcttcctgtatgtcattcgacagggaggacaagacatacattcatatcatcgaccagaggaccaggaagcctgtgcctaccaagttctacacagatcccatggtggtcttccatcatgtcaatgcctacgaggaggacggctgtgtgctgtttgatgtgatcgcctatgaggacagcagcctctatcagctcttctacctggccaacctgaacaaggacttcgaggagaagtccaggctgacctcagtgcctaccctcaggaggtttgctgtgcccctccatgtggacaaggatgcagaagtgggctcaaatttagtcaaggtgtcatctacaactgcaacagccctgaaggagaaagacggccatgtctattgccagcccgaggtcctctacgaaggcctagagctccctcggataaattatgcttacaacgggaagccatatcgctacatctttgcagctgaagtacagtggagtccagtcccaaccaagatactgaaatatgacattctcacaaagtcctccttaaagtggtctgaggagagctgctggccagcagagcctctgtttgttcccacgccaggtgcgagggatgaagatgatggagtcattttatcagccatcgtctctacggatccccaaaagctgccttttttactcattctggatgccaaaagttttgcggaactggctcgcgcctctgttgatgcggacatgcacctggaccttcatggtttatttatcccagatgcagactggaatgcagtgaagcagactccagctgaaacgcaagaggttgaaaactcagatcatcccacagatccgacagcaccagaactgagccacagtgaaaatgacttcacagcgggtcatggtggctcaagtctttaa
BBa_S04629_sequence
1
aaagaggagaaatactagatggagataatatttggccagaataagaaagaacagctggagccagttcaggccaaagtgacaggcagcattccagcatggctgcaggggaccctgctccgaaacgggcccgggatgcacacagtgggagagagcaagtacaaccattggtttgatggcctggcccttctccacagtttctccatcagagatggggaggtcttctacaggagcaaatacctgcagagtgacacctacatcgccaacattgaggccaacagaatcgtggtgtctgagttcggaaccatggcctacccggacccctgcaaaaacatcttttccaaagctttctcctacttgtctcacaccatccccgacttcacagacaactgtctgatcaacatcatgaaatgtggagaagacttctatgcaaccacggagaccaactacatcaggaaaatcgacccccagaccctagagactttggagaaggttgattaccggaagtatgtggcggtaaacctggctacctcgcaccctcattatgacgaggctgggaatgtccttaacatgggcacatccgtcgtggacaaagggaggacaaaatacgtgatatttaagatccctgccacagtgccagacagcaagaagaaagggaagagtcccgtgaagcacgcggaagttttctgctccatttcctcccgctcgctgctctctcccagctactaccacagctttggtgtcacggagaactatgtggtgtttctggagcagccttttaagttggatatcctcaagatggccaccgcatacatgaggggagtgagctgggcttcctgtatgtcattcgacagggaggacaagacatacattcatatcatcgaccagaggaccaggaagcctgtgcctaccaagttctacacagatcccatggtggtcttccatcatgtcaatgcctacgaggaggacggctgtgtgctgtttgatgtgatcgcctatgaggacagcagcctctatcagctcttctacctggccaacctgaacaaggacttcgaggagaagtccaggctgacctcagtgcctaccctcaggaggtttgctgtgcccctccatgtggacaaggatgcagaagtgggctcaaatttagtcaaggtgtcatctacaactgcaacagccctgaaggagaaagacggccatgtctattgccagcccgaggtcctctacgaaggcctagagctccctcggataaattatgcttacaacgggaagccatatcgctacatctttgcagctgaagtacagtggagtccagtcccaaccaagatactgaaatatgacattctcacaaagtcctccttaaagtggtctgaggagagctgctggccagcagagcctctgtttgttcccacgccaggtgcgagggatgaagatgatggagtcattttatcagccatcgtctctacggatccccaaaagctgccttttttactcattctggatgccaaaagttttgcggaactggctcgcgcctctgttgatgcggacatgcacctggaccttcatggtttatttatcccagatgcagactggaatgcagtgaagcagactccagctgaaacgcaagaggttgaaaactcagatcatcccacagatccgacagcaccagaactgagccacagtgaaaatgacttcacagcgggtcatggtggctcaagtctttaa
BBa_B0034_sequence
1
aaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z