BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_S04629 1 BBa_S04629 B0034:K572009 2011-10-03T11:00:00Z 2015-05-08T01:14:43Z false false _9_ 0 7681 9 Not in stock false false Varun Chokshi component2151863 1 BBa_K572009 component2151859 1 BBa_B0034 annotation2151859 1 BBa_B0034 range2151859 1 1 12 annotation2151863 1 BBa_K572009 range2151863 1 19 1719 BBa_K572009 1 BBa_K572009 Mouse Beta Carotene Dioxygenase 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z Source Organism : Marine gamma proteobacterium EBAC31A08. We would like to thank Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea for sending us the plasmid (pKJ900) with dioxygenase gene. .. false true _743_ 0 7684 9 It's complicated false Forward Primer : 5???TTTACTACGAATTCGCGGCCGCTTCTAGATGGAGATAATATTTG 3??? Reverse Primer : 5???AATCGAACctgcagcggccgctactagtaTTAAAGACTTGAG 3??? Polymerase Chain reactions didn't work for a long time at various temperature conditions. Finally, increasing Magnesium Sulphate in the reaction resulted in bringing about the amplification. The following is the optimized PCR Setup : Template (116 ng/ul) = 0.25 ul 10mM dNTP = 1.0 ul 10x Pfu Buffer (without Magnesium Sulphate) = 2.0 ul + Primer (10 uM) = 1.0 ul - Primer (10 uM) = 1.0 ul Magnesium Sulphate - final concentration of 2.5 - 3 mM Pfu Pol = 1.25 u Nuclease Free Water - make the volume to 20ul false Kousik Sundararajan annotation2151502 1 Start Codon range2151502 1 1 3 annotation2151504 1 Stop Codon range2151504 1 1699 1701 annotation2151503 1 Bc_dioxygenase range2151503 1 1 1701 BBa_K572009_sequence 1 atggagataatatttggccagaataagaaagaacagctggagccagttcaggccaaagtgacaggcagcattccagcatggctgcaggggaccctgctccgaaacgggcccgggatgcacacagtgggagagagcaagtacaaccattggtttgatggcctggcccttctccacagtttctccatcagagatggggaggtcttctacaggagcaaatacctgcagagtgacacctacatcgccaacattgaggccaacagaatcgtggtgtctgagttcggaaccatggcctacccggacccctgcaaaaacatcttttccaaagctttctcctacttgtctcacaccatccccgacttcacagacaactgtctgatcaacatcatgaaatgtggagaagacttctatgcaaccacggagaccaactacatcaggaaaatcgacccccagaccctagagactttggagaaggttgattaccggaagtatgtggcggtaaacctggctacctcgcaccctcattatgacgaggctgggaatgtccttaacatgggcacatccgtcgtggacaaagggaggacaaaatacgtgatatttaagatccctgccacagtgccagacagcaagaagaaagggaagagtcccgtgaagcacgcggaagttttctgctccatttcctcccgctcgctgctctctcccagctactaccacagctttggtgtcacggagaactatgtggtgtttctggagcagccttttaagttggatatcctcaagatggccaccgcatacatgaggggagtgagctgggcttcctgtatgtcattcgacagggaggacaagacatacattcatatcatcgaccagaggaccaggaagcctgtgcctaccaagttctacacagatcccatggtggtcttccatcatgtcaatgcctacgaggaggacggctgtgtgctgtttgatgtgatcgcctatgaggacagcagcctctatcagctcttctacctggccaacctgaacaaggacttcgaggagaagtccaggctgacctcagtgcctaccctcaggaggtttgctgtgcccctccatgtggacaaggatgcagaagtgggctcaaatttagtcaaggtgtcatctacaactgcaacagccctgaaggagaaagacggccatgtctattgccagcccgaggtcctctacgaaggcctagagctccctcggataaattatgcttacaacgggaagccatatcgctacatctttgcagctgaagtacagtggagtccagtcccaaccaagatactgaaatatgacattctcacaaagtcctccttaaagtggtctgaggagagctgctggccagcagagcctctgtttgttcccacgccaggtgcgagggatgaagatgatggagtcattttatcagccatcgtctctacggatccccaaaagctgccttttttactcattctggatgccaaaagttttgcggaactggctcgcgcctctgttgatgcggacatgcacctggaccttcatggtttatttatcccagatgcagactggaatgcagtgaagcagactccagctgaaacgcaagaggttgaaaactcagatcatcccacagatccgacagcaccagaactgagccacagtgaaaatgacttcacagcgggtcatggtggctcaagtctttaa BBa_S04629_sequence 1 aaagaggagaaatactagatggagataatatttggccagaataagaaagaacagctggagccagttcaggccaaagtgacaggcagcattccagcatggctgcaggggaccctgctccgaaacgggcccgggatgcacacagtgggagagagcaagtacaaccattggtttgatggcctggcccttctccacagtttctccatcagagatggggaggtcttctacaggagcaaatacctgcagagtgacacctacatcgccaacattgaggccaacagaatcgtggtgtctgagttcggaaccatggcctacccggacccctgcaaaaacatcttttccaaagctttctcctacttgtctcacaccatccccgacttcacagacaactgtctgatcaacatcatgaaatgtggagaagacttctatgcaaccacggagaccaactacatcaggaaaatcgacccccagaccctagagactttggagaaggttgattaccggaagtatgtggcggtaaacctggctacctcgcaccctcattatgacgaggctgggaatgtccttaacatgggcacatccgtcgtggacaaagggaggacaaaatacgtgatatttaagatccctgccacagtgccagacagcaagaagaaagggaagagtcccgtgaagcacgcggaagttttctgctccatttcctcccgctcgctgctctctcccagctactaccacagctttggtgtcacggagaactatgtggtgtttctggagcagccttttaagttggatatcctcaagatggccaccgcatacatgaggggagtgagctgggcttcctgtatgtcattcgacagggaggacaagacatacattcatatcatcgaccagaggaccaggaagcctgtgcctaccaagttctacacagatcccatggtggtcttccatcatgtcaatgcctacgaggaggacggctgtgtgctgtttgatgtgatcgcctatgaggacagcagcctctatcagctcttctacctggccaacctgaacaaggacttcgaggagaagtccaggctgacctcagtgcctaccctcaggaggtttgctgtgcccctccatgtggacaaggatgcagaagtgggctcaaatttagtcaaggtgtcatctacaactgcaacagccctgaaggagaaagacggccatgtctattgccagcccgaggtcctctacgaaggcctagagctccctcggataaattatgcttacaacgggaagccatatcgctacatctttgcagctgaagtacagtggagtccagtcccaaccaagatactgaaatatgacattctcacaaagtcctccttaaagtggtctgaggagagctgctggccagcagagcctctgtttgttcccacgccaggtgcgagggatgaagatgatggagtcattttatcagccatcgtctctacggatccccaaaagctgccttttttactcattctggatgccaaaagttttgcggaactggctcgcgcctctgttgatgcggacatgcacctggaccttcatggtttatttatcccagatgcagactggaatgcagtgaagcagactccagctgaaacgcaagaggttgaaaactcagatcatcccacagatccgacagcaccagaactgagccacagtgaaaatgacttcacagcgggtcatggtggctcaagtctttaa BBa_B0034_sequence 1 aaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z