BBa_J176041 1 scar Silver scar 2011-10-04T11:00:00Z 2015-08-31T04:08:34Z This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard). Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard). false false _863_ 0 1144 61 Not in stock false The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts. false Karmella Haynes BBa_J176042 1 polyA polyA 2011-10-06T11:00:00Z 2015-08-31T04:08:34Z Originally from the Silver Lab BioBrick collection. Biological source unknown. This part was created by David Drubin. Polyadenylation signal for mammalian genes false false _863_ 0 1144 61 Not in stock false This is the first version of polyA that we used to build many of our mammalian parts, until we switched to a streamlined, shorter polyA (polyA2, BBa_J176009). false Karmella Haynes annotation2156673 1 questionable base call range2156673 1 128 128 BBa_J176021 1 HS4 HS4 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA chicken beta-globin gene insulator false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_S04691 1 BBa_S04691 J176025:J176009 2011-10-06T11:00:00Z 2015-05-08T01:14:44Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2156724 1 BBa_J176041 component2156721 1 BBa_J176042 component2156718 1 BBa_J176025 component2156719 1 BBa_J176041 component2156722 1 BBa_J176041 component2156723 1 BBa_J176021 component2156725 1 BBa_J176021 annotation2156725 1 BBa_J176021 range2156725 1 1014 1353 annotation2156724 1 BBa_J176041 range2156724 1 1008 1013 annotation2156723 1 BBa_J176021 range2156723 1 668 1007 annotation2156721 1 BBa_J176042 range2156721 1 194 661 annotation2156719 1 BBa_J176041 range2156719 1 188 193 annotation2156722 1 BBa_J176041 range2156722 1 662 667 annotation2156718 1 BBa_J176025 range2156718 1 1 187 BBa_J176025 1 miRts luc miRts luc 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA miRNA target for luciferase RNAi false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2166811 1 repeat 5 range2166811 1 135 153 annotation2166809 1 repeat 3 range2166809 1 82 100 annotation2166807 1 repeat 1 range2166807 1 28 46 annotation2166808 1 repeat 2 range2166808 1 55 73 annotation2166810 1 repeat 4 range2166810 1 109 127 BBa_J176042_sequence 1 agacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagctt BBa_S04691_sequence 1 atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagaagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagcttactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgccactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgcc BBa_J176025_sequence 1 atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgc BBa_J176021_sequence 1 ttaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgcc BBa_J176041_sequence 1 actaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z