LOCUS       BBa_S04735              3244 bp    DNA     linear       25-Nov-2024
DEFINITION  J176041:J176009
ACCESSION   BBa_S04735
VERSION     BBa_S04735.1  
FEATURES             Location/Qualifiers
     misc_feature    253..253
                     /label=mutated SpeI
     misc_feature    511..511
                     /label=mutated PstI
     promoter        1..516
                     /label=BBa_J176028
     misc_feature    517..522
                     /label=BBa_J176041
     RBS             523..540
                     /label=BBa_J176012
     misc_feature    541..546
                     /label=BBa_J176041
     misc_feature    547..1005
                     /label=BBa_J176020
     misc_feature    1006..1011
                     /label=BBa_J176041
     misc_feature    1012..1545
                     /label=BBa_J176018
     misc_feature    1546..1551
                     /label=BBa_J176041
     misc_feature    1552..2256
                     /label=BBa_J176005
     misc_feature    2257..2262
                     /label=BBa_J176041
     misc_feature    2263..2967
                     /label=BBa_J176005
     misc_feature    2968..2973
                     /label=BBa_J176041
     misc_feature    2974..2994
                     /label=BBa_J176008
     misc_feature    2995..3000
                     /label=BBa_J176041
     terminator      3001..3013
                     /label=BBa_J176016
     misc_feature    1..3013
                     /label=BBa_S04732
     misc_feature    3014..3019
                     /label=BBa_J176041
     misc_feature    3020..3244
                     /label=BBa_J176009
ORIGIN
        1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
       61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
      121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
      181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
      241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
      301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
      361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
      421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
      481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacccgccgc caccatggag
      541 actagaatga agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag
      601 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt
      661 cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa
      721 tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga agaccttgac
      781 atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta
      841 caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg
      901 cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac
      961 aaaggtcaaa gacagttgac tgtatcgccg gaatttccgg ggatcactag agcatccaac
     1021 ttgaacattt cgagaaagct taccatatca accccatcat gctctttcga aaattcaaat
     1081 agcacatcca ttccttcgcc cgcttcctca tctcaaagcc acactccaat gagaaacatg
     1141 agctcactct ctgataacag cgttttcagc cggaatatgg aacaatcatc accaatcact
     1201 ccaagtatgt accaatttgg tcagcagcag tcaaacagta tatgtggtag caccgttagt
     1261 gtgaatagtc tggtgaatac aaataacaaa caaaggatct acgaacaaat cacgggtcct
     1321 aacagcaata acgcaaccaa tgattatatt gatttgctaa acctaaatga gtctaacaag
     1381 gaaaaccaaa atcccgcaac ggcgcattac ctcaatgggg gcccacccaa gacaagcttc
     1441 attaaccatg gaatgttccc ctcgccaact gggaccataa atagcggtaa atctagcagt
     1501 gcctcatctt taatttcttt tggtatgggc aatacccaag taataactag agtgagcaag
     1561 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1621 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1681 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1741 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1801 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1861 gaggacggcg gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc
     1921 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1981 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     2041 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     2101 acctacaagg ccaagaagcc cgtgcagcta cccggcgcct acaacgtcaa catcaagttg
     2161 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     2221 cgccactcca ccggcggcat ggacgagctg tacaagacta gagtgagcaa gggcgaggag
     2281 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     2341 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     2401 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     2461 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     2521 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     2581 ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg
     2641 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     2701 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     2761 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     2821 gccaagaagc ccgtgcagct acccggcgcc tacaacgtca acatcaagtt ggacatcacc
     2881 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     2941 accggcggca tggacgagct gtacaagact agacccaaga aaaagcgcaa ggtaactaga
     3001 gcgtaaagct gagactagac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3061 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3121 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3181 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3241 atgg
//