BBa_J176131 1 PLrigid PLrigid 2011-10-27T11:00:00Z 2015-08-31T04:08:35Z TBA (EAAAR)4 peptide linker, rigid; alpha helix secondary structure; see Yan et al. 2007 false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2179297 1 EAAAR range2179297 1 16 30 annotation2179298 1 EAAAR range2179298 1 31 45 annotation2179296 1 EAAAR range2179296 1 1 15 annotation2179299 1 EAAAR range2179299 1 46 60 BBa_J176041 1 scar Silver scar 2011-10-04T11:00:00Z 2015-08-31T04:08:34Z This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard). Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard). false false _863_ 0 1144 61 Not in stock false The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts. false Karmella Haynes BBa_S04854 1 BBa_S04854 J176000:J176041 2011-11-11T12:00:00Z 2015-05-08T01:14:46Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2374916 1 BBa_J176041 component2374922 1 BBa_J176041 component2374915 1 BBa_J176000 component2374923 1 BBa_J176002 component2374927 1 BBa_J176036 component2374921 1 BBa_J176131 component2374924 1 BBa_J176041 component2374925 1 BBa_J176005 component2374926 1 BBa_J176041 annotation2374924 1 BBa_J176041 range2374924 1 445 450 annotation2374921 1 BBa_J176131 range2374921 1 193 252 annotation2374922 1 BBa_J176041 range2374922 1 253 258 annotation2374926 1 BBa_J176041 range2374926 1 1156 1161 annotation2374927 1 BBa_J176036 range2374927 1 1162 1701 annotation2374916 1 BBa_J176041 range2374916 1 187 192 annotation2374923 1 BBa_J176002 range2374923 1 259 444 annotation2374915 1 BBa_J176000 range2374915 1 1 186 annotation2374925 1 BBa_J176005 range2374925 1 451 1155 BBa_J176005 1 mCh mCh 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Humanized mCherry fluorescent protein false false _863_ 0 10865 9 It's complicated In stock false TBA false Karmella Haynes BBa_J176036 1 BBa_J176036 SP1A 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA activation domain A from SP1; Emamai et al. 1995 false false _863_ 0 10865 9 Not in stock false TBA false Brady Laughlin BBa_J176002 1 fshPCD fshPCD 2011-07-02T11:00:00Z 2015-08-31T04:08:33Z Drosophila Pc protein amino acids 16-78. Human codon optimized sequence was synthesized by Genscript. Polycomb chromodomain from the Drosophila (fruit fly) Pc protein (a.a. 16-78). false false _863_ 0 1144 61 Not in stock false Human codon optimized for expression in human cell culture. false Karmella Haynes BBa_J176000 1 hPCD hPCD 2011-06-23T11:00:00Z 2015-08-31T04:08:33Z Synthesized by GenScript. Human Polycomb chromodomain from the CBX8 protein (a.a. 1-63). See Haynes and Silver, JBC, 2011 false false _863_ 0 1144 61 Not in stock false The sequence was not modified. false Karmella Haynes BBa_J176036_sequence 1 ggcccgagtcagtcagggggaacaggtgagcttgacctcacagccacacaactttcacagggtgccaatggctggcagatcatctcttcctcctctggggctacccctacctcaaaggaacagagtggcagcagtaccaatggcagcaatggcagtgagtcttccaagaatcgcacagtctctggtgggcagtatgttgtggctgccgctcccaacttacagaaccagcgagttctgacaggactacctggagtgatgcctaatattcagtatcaagtaatcccacagttccagaccgttgatgggcaacagctgcaatttgctgccactggggcccaagtgcagcaggatggttctggtcaaatacagatcataccaggtgcaaaccaacagattatcacaaatcgaggaagtggaggcaacatcattgctgctatgccaaacctactccagcaggctgtccccctccaaggcctggctaataatgtactctcaggacagactcagtatgtgaccaatgtaccagtggccctgaatggg BBa_J176131_sequence 1 gaagctgctgcaagagaagctgcagctagggaggctgcagctagggaggctgctgcaaga BBa_S04854_sequence 1 atggagctttcagcggtgggggagcgggtgttcgcggccgaagccctcctgaagcggcgcatacggaaaggacgcatggaatacctcgtgaaatggaagggatggtcgcagaagtacagcacatgggaaccggaggaaaacatcctggatgctcgcttgctcgcagcctttgaggaaagggaaagaactagagaagctgctgcaagagaagctgcagctagggaggctgcagctagggaggctgctgcaagaactagagaggagctgagcgcggtgggagagcaggtctttgacgccgagtgtatcctcaacaaacgcacgaggaagggcaaactggagtatctggtcaagtggagaggatggtcgtccaagcataacagttgggaacctcaggaaaaccttcttgacccgagactcttggtggcatttaacaagagggagcagactagagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagactagaggcccgagtcagtcagggggaacaggtgagcttgacctcacagccacacaactttcacagggtgccaatggctggcagatcatctcttcctcctctggggctacccctacctcaaaggaacagagtggcagcagtaccaatggcagcaatggcagtgagtcttccaagaatcgcacagtctctggtgggcagtatgttgtggctgccgctcccaacttacagaaccagcgagttctgacaggactacctggagtgatgcctaatattcagtatcaagtaatcccacagttccagaccgttgatgggcaacagctgcaatttgctgccactggggcccaagtgcagcaggatggttctggtcaaatacagatcataccaggtgcaaaccaacagattatcacaaatcgaggaagtggaggcaacatcattgctgctatgccaaacctactccagcaggctgtccccctccaaggcctggctaataatgtactctcaggacagactcagtatgtgaccaatgtaccagtggccctgaatggg BBa_J176002_sequence 1 gaggagctgagcgcggtgggagagcaggtctttgacgccgagtgtatcctcaacaaacgcacgaggaagggcaaactggagtatctggtcaagtggagaggatggtcgtccaagcataacagttgggaacctcaggaaaaccttcttgacccgagactcttggtggcatttaacaagagggagcag BBa_J176005_sequence 1 gtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag BBa_J176041_sequence 1 actaga BBa_J176000_sequence 1 atggagctttcagcggtgggggagcgggtgttcgcggccgaagccctcctgaagcggcgcatacggaaaggacgcatggaatacctcgtgaaatggaagggatggtcgcagaagtacagcacatgggaaccggaggaaaacatcctggatgctcgcttgctcgcagcctttgaggaaagggaaaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z