BBa_J176131 1 PLrigid PLrigid 2011-10-27T11:00:00Z 2015-08-31T04:08:35Z TBA (EAAAR)4 peptide linker, rigid; alpha helix secondary structure; see Yan et al. 2007 false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2179297 1 EAAAR range2179297 1 16 30 annotation2179296 1 EAAAR range2179296 1 1 15 annotation2179298 1 EAAAR range2179298 1 31 45 annotation2179299 1 EAAAR range2179299 1 46 60 BBa_J176041 1 scar Silver scar 2011-10-04T11:00:00Z 2015-08-31T04:08:34Z This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard). Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard). false false _863_ 0 1144 61 Not in stock false The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts. false Karmella Haynes BBa_J176005 1 mCh mCh 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Humanized mCherry fluorescent protein false false _863_ 0 10865 9 It's complicated In stock false TBA false Karmella Haynes BBa_J176001 1 flyPCD flyPCD 2011-07-01T11:00:00Z 2015-08-31T04:08:33Z Danio rerio (zebrafish) CBX2 amino acids 2-64. The human codon-optimized coding sequence was synthesized by Genscript. Amino acids 2-64 from the zebrafish CBX2 protein. This is the conserved Polycomb chromodomain predicted to bind trimethylated histone H3 lysine 27 in chromatin. false false _863_ 0 1144 61 Not in stock false Codons were optimized for expression in cultured human cells. false Karmella Haynes BBa_J176013 1 VP64 VP64 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Tetrameric VP16 transc. activator domain false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2166132 1 VP16 range2166132 1 118 150 annotation2166129 1 VP16 range2166129 1 1 33 annotation2166130 1 VP16 range2166130 1 40 72 annotation2166131 1 VP16 range2166131 1 79 111 BBa_S04967 1 BBa_S04967 J176001:J176041 2011-11-14T12:00:00Z 2015-05-08T01:14:47Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2376402 1 BBa_J176013 component2376395 1 BBa_J176041 component2376386 1 BBa_J176001 component2376394 1 BBa_J176000 component2376393 1 BBa_J176041 component2376387 1 BBa_J176041 component2376397 1 BBa_J176041 component2376392 1 BBa_J176131 component2376396 1 BBa_J176005 annotation2376393 1 BBa_J176041 range2376393 1 253 258 annotation2376402 1 BBa_J176013 range2376402 1 1162 1311 annotation2376386 1 BBa_J176001 range2376386 1 1 186 annotation2376394 1 BBa_J176000 range2376394 1 259 444 annotation2376397 1 BBa_J176041 range2376397 1 1156 1161 annotation2376392 1 BBa_J176131 range2376392 1 193 252 annotation2376395 1 BBa_J176041 range2376395 1 445 450 annotation2376396 1 BBa_J176005 range2376396 1 451 1155 annotation2376387 1 BBa_J176041 range2376387 1 187 192 BBa_J176000 1 hPCD hPCD 2011-06-23T11:00:00Z 2015-08-31T04:08:33Z Synthesized by GenScript. Human Polycomb chromodomain from the CBX8 protein (a.a. 1-63). See Haynes and Silver, JBC, 2011 false false _863_ 0 1144 61 Not in stock false The sequence was not modified. false Karmella Haynes BBa_J176001_sequence 1 aatgcgaccgacgatccagtcgatctcgtgtacgcggctgagaaaatcatccaaaagcgcgttaagaagggcgtcgtggagtaccgtgtcaagtggaagggctggaaccagcgctacaacacctgggaaccggaggtaaacatcctggatcgccgcctcatcgacatctacgaacaaacgaacaaa BBa_J176131_sequence 1 gaagctgctgcaagagaagctgcagctagggaggctgcagctagggaggctgctgcaaga BBa_J176013_sequence 1 gacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttg BBa_J176005_sequence 1 gtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag BBa_J176041_sequence 1 actaga BBa_S04967_sequence 1 aatgcgaccgacgatccagtcgatctcgtgtacgcggctgagaaaatcatccaaaagcgcgttaagaagggcgtcgtggagtaccgtgtcaagtggaagggctggaaccagcgctacaacacctgggaaccggaggtaaacatcctggatcgccgcctcatcgacatctacgaacaaacgaacaaaactagagaagctgctgcaagagaagctgcagctagggaggctgcagctagggaggctgctgcaagaactagaatggagctttcagcggtgggggagcgggtgttcgcggccgaagccctcctgaagcggcgcatacggaaaggacgcatggaatacctcgtgaaatggaagggatggtcgcagaagtacagcacatgggaaccggaggaaaacatcctggatgctcgcttgctcgcagcctttgaggaaagggaaagaactagagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagactagagacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttgggttctgacgctttggacgacttcgacttggacatgttg BBa_J176000_sequence 1 atggagctttcagcggtgggggagcgggtgttcgcggccgaagccctcctgaagcggcgcatacggaaaggacgcatggaatacctcgtgaaatggaagggatggtcgcagaagtacagcacatgggaaccggaggaaaacatcctggatgctcgcttgctcgcagcctttgaggaaagggaaaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z