BBa_J119026
1
BBa_J119026
BD10 bicistronic translational junction - Low efficiency
2011-12-21T12:00:00Z
2015-10-01T11:43:18Z
Synthetic DNA
The BD10 bicistronic translational junction was engineered by Vivek Mutalik and The BIOFAB Team at biofab.org. It includes an RBS that allows translation of a leader polypeptide. The leader has a second RBS embedded in it. The stop codon for the leader polypeptide overlaps with the start codon for the gene of interest.
false
false
_613_
606
606
61
Not in stock
false
The bicistronic translational junction was designed by Team Leader Vivek Mutalik at BioFAB [1]. It is is intended to give reproducible and consistent expression for various genes of interest.
false
Vivek Mutalik
annotation2167453
1
Start
range2167453
1
33
35
annotation2167454
1
Start
range2167454
1
86
88
annotation2167452
1
Stop
range2167452
1
84
86
annotation2167451
1
GOI RBS
range2167451
1
71
79
annotation2167450
1
leader RBS
range2167450
1
19
27
BBa_S05014
1
BBa_S05014
J119033:J119026
2012-01-09T12:00:00Z
2015-05-08T01:14:48Z
false
false
_61_
0
201
61
Not in stock
false
false
Malcolm Campbell
component2169250
1
BBa_J119033
component2169256
1
BBa_J119026
annotation2169256
1
BBa_J119026
range2169256
1
37
124
annotation2169250
1
BBa_J119033
range2169250
1
1
36
BBa_J119033
1
BBa_J119033
P14 Promoter - Medium efficiency
2011-12-22T12:00:00Z
2015-10-01T11:41:31Z
Synthetic DNA.
P14 promoter engineered by Vivek Mutalik and The BIOFAB Team at biofab.org. Derived from pTrc promoter.
false
false
_613_
606
606
61
Not in stock
false
Designed by Team Leader Vivek Mutalik at BioFAB [http://www.biofab.org] for use in testing bicistronic translational junctions (eg. http://partsregistry.org/Part:BBa_J119024).
false
Vivek Mutalik
BBa_S05014_sequence
1
ttgacaattaatcatccggctcgtataatgtgtggagggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcggaggatcgtttctaatg
BBa_J119026_sequence
1
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcggaggatcgtttctaatg
BBa_J119033_sequence
1
ttgacaattaatcatccggctcgtataatgtgtgga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z