BBa_S05031 1 BBa_S05031 J119030:J119028 2012-01-09T12:00:00Z 2015-05-08T01:14:48Z false false _61_ 0 201 61 Not in stock false false Malcolm Campbell component2169522 1 BBa_J119028 component2169516 1 BBa_J119030 annotation2169516 1 BBa_J119030 range2169516 1 1 36 annotation2169522 1 BBa_J119028 range2169522 1 37 124 BBa_J119030 1 BBa_J119030 P4 Promoter - Lowest efficiency 2011-12-22T12:00:00Z 2015-10-01T11:39:34Z Synthetic DNA. P4 promoter engineered by Vivek Mutalik and The BIOFAB Team at biofab.org. false false _613_ 606 606 61 Not in stock false Designed by Team Leader Vivek Mutalik at BioFAB [http://www.biofab.org] for use in testing bicistronic translational junctions (eg. http://partsregistry.org/Part:BBa_J119024). false Vivek Mutalik annotation2167467 1 -35 range2167467 1 1 6 annotation2167468 1 -10 range2167468 1 24 36 BBa_J119028 1 BBa_J119028 BD24 bicistronic translational junction - Highest efficiency 2011-12-21T12:00:00Z 2015-10-01T11:44:13Z Synthetic DNA. The BD24 bicistronic translational junction was engineered by Vivek Mutalik and The BIOFAB Team at biofab.org. It includes an RBS that allows translation of a leader polypeptide. The leader has a second RBS embedded in it. The stop codon for the leader polypeptide overlaps with the start codon for the gene of interest. false false _613_ 606 606 61 Not in stock false The bicistronic translational junction was designed by Team Leader Vivek Mutalik at BioFAB [http://www.biofab.org]. It is is intended to give reproducible and consistent expression for various genes of interest. false Vivek Mutalik annotation2167462 1 Stop range2167462 1 84 86 annotation2167460 1 Start range2167460 1 33 35 annotation2167464 1 GOI RBS range2167464 1 71 79 annotation2167463 1 leader RBS range2167463 1 19 27 annotation2167461 1 Start range2167461 1 86 88 BBa_J119028_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgatggacggtttctaatg BBa_S05031_sequence 1 ttgacatcaggaaaatttttctgtataatgtgtggagggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgatggacggtttctaatg BBa_J119030_sequence 1 ttgacatcaggaaaatttttctgtataatgtgtgga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z