BBa_S05032
type: ComponentDefinition
persistent identity: BBa S05032
display id: BBa_S05032
version: 1
was generated by: iGEM to SBOL conversion
description: K559011:K112808
created: 2012-07-09T11:00:00Z
modified: 2015-05-08T01:14:48Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 12861
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Michael Kenton
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K112808
component: BBa_K559011
sequence annotation: BBa_K559011
sequence annotation: BBa_K112808
sequence: https://synbiohub.org/public/igem/BBa_S05032_sequence/1

BBa_K112808
type: Component
persistent identity: component2177616
display id: component2177616
version: 1
top level: BBa_S05032
definition: BBa_K112808
access: public

BBa_K559011
type: Component
persistent identity: component2177600
display id: component2177600
version: 1
top level: BBa_S05032
definition: Pgad
access: public

BBa_K559011
type: SequenceAnnotation
persistent identity: annotation2177600
display id: annotation2177600
version: 1
top level: BBa_S05032
location: https://synbiohub.org/public/igem/BBa_S05032/annotation2177600/range2177600/1
component: BBa_K559011

https://synbiohub.org/public/igem/BBa_S05032/annotation2177600/range2177600/1
type: Range
persistent identity: range2177600
display id: range2177600
version: 1
top level: BBa_S05032
direction: direction/reverse
start: 1
end: 1251
orientation: inline

BBa_K112808
type: SequenceAnnotation
persistent identity: annotation2177616
display id: annotation2177616
version: 1
top level: BBa_S05032
location: https://synbiohub.org/public/igem/BBa_S05032/annotation2177616/range2177616/1
component: BBa_K112808

https://synbiohub.org/public/igem/BBa_S05032/annotation2177616/range2177616/1
type: Range
persistent identity: range2177616
display id: range2177616
version: 1
top level: BBa_S05032
direction: direction/reverse
start: 1260
end: 3044
orientation: inline

Pgad
type: ComponentDefinition
persistent identity: BBa K559011
display id: BBa_K559011
version: 1
was generated by: iGEM to SBOL conversion
description: Pgad - Intracellular Chloride-Sensing Cassette
created: 2011-10-27T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: The gene sequence was obtained from the cassette from bp 821 to 2071 of GenBank sequence AF005098, which includes PgadR, gadR, Pgad and the starting codon ATG.
owned by: james
owned by: myers
mutable description: Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment. In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad2. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated.
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Synthesized part by GenScript,all the illegal sites for RFC 10 (EcoRI, XbaI, SpeI, PstI) have been substituted by other codon producing the same amino acid in E. coli.
star: false
creator: Jacky, Fong Chuen, Loo
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: PgadR
sequence annotation: RBS_gadR
sequence annotation: gadR
sequence annotation: Pgad
sequence annotation: RBS_gadC
sequence annotation: gadC Start
sequence: https://synbiohub.org/public/igem/BBa_K559011_sequence/1

PgadR
type: SequenceAnnotation
persistent identity: annotation2160937
display id: annotation2160937
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160937/range2160937/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K559011/annotation2160937/range2160937/1
type: Range
persistent identity: range2160937
display id: range2160937
version: 1
top level: Pgad
direction: direction/forward
start: 141
end: 158
orientation: inline

RBS_gadR
type: SequenceAnnotation
persistent identity: annotation2160941
display id: annotation2160941
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160941/range2160941/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K559011/annotation2160941/range2160941/1
type: Range
persistent identity: range2160941
display id: range2160941
version: 1
top level: Pgad
direction: direction/forward
start: 264
end: 269
orientation: inline

gadR
type: SequenceAnnotation
persistent identity: annotation2160938
display id: annotation2160938
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160938/range2160938/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K559011/annotation2160938/range2160938/1
type: Range
persistent identity: range2160938
display id: range2160938
version: 1
top level: Pgad
direction: direction/forward
start: 275
end: 1105
orientation: inline

Pgad
type: SequenceAnnotation
persistent identity: annotation2160939
display id: annotation2160939
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160939/range2160939/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K559011/annotation2160939/range2160939/1
type: Range
persistent identity: range2160939
display id: range2160939
version: 1
top level: Pgad
direction: direction/forward
start: 1163
end: 1181
orientation: inline

RBS_gadC
type: SequenceAnnotation
persistent identity: annotation2160940
display id: annotation2160940
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160940/range2160940/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K559011/annotation2160940/range2160940/1
type: Range
persistent identity: range2160940
display id: range2160940
version: 1
top level: Pgad
direction: direction/forward
start: 1237
end: 1245
orientation: inline

gadC Start
type: SequenceAnnotation
persistent identity: annotation2160936
display id: annotation2160936
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160936/range2160936/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K559011/annotation2160936/range2160936/1
type: Range
persistent identity: range2160936
display id: range2160936
version: 1
top level: Pgad
direction: direction/forward
start: 1249
end: 1251
orientation: inline

BBa_K112808
type: ComponentDefinition
persistent identity: BBa K112808
display id: BBa_K112808
version: 1
was generated by: iGEM to SBOL conversion
description: Enterobacteria phage T4 Lysis Device - no promoter
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Pubmed Accession Number: NC_000866 Enterobacteria phage T4
owned by: james
owned by: myers
mutable description: This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin. Various promoters can be installed with this device to control the lysis of bacteria. Antiholin has its own constitutive promoter to prevent the formation of holin multimers from basal expression. When the device is off, higher expression of antiholin compared to holin is required for better stability of the device.
discontinued: false
dominant: true
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: [Pcon].[Antiholin].[terminator] was assembled first, and then assembled with [T4 holin].[terminator]; Constitutive expression of antiholin is required before assembling holin.
star: true
creator: Jin Huh
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J23116
component: BBa_B0012
component: BBa_B0010
component: BBa_K112807
component: BBa_K112805
component: BBa_K112806
sequence annotation: BBa_K112805
sequence annotation: BBa_K112806
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23116
sequence annotation: BBa_K112807
sequence: https://synbiohub.org/public/igem/BBa_K112808_sequence/1

BBa_J23116
type: Component
persistent identity: component1999030
display id: component1999030
version: 1
top level: BBa_K112808
definition: BBa_J23116
access: public

BBa_B0012
type: Component
persistent identity: component1999026
display id: component1999026
version: 1
top level: BBa_K112808
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1999033
display id: component1999033
version: 1
top level: BBa_K112808
definition: BBa_B0010
access: public

BBa_K112807
type: Component
persistent identity: component1999032
display id: component1999032
version: 1
top level: BBa_K112808
definition: BBa_K112807
access: public

BBa_K112805
type: Component
persistent identity: component1999021
display id: component1999021
version: 1
top level: BBa_K112808
definition: BBa_K112805
access: public

BBa_B0010
type: Component
persistent identity: component1999024
display id: component1999024
version: 1
top level: BBa_K112808
definition: BBa_B0010
access: public

BBa_K112806
type: Component
persistent identity: component1999023
display id: component1999023
version: 1
top level: BBa_K112808
definition: BBa_K112806
access: public

BBa_K112805
type: SequenceAnnotation
persistent identity: annotation1999021
display id: annotation1999021
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999021/range1999021/1
component: BBa_K112805

https://synbiohub.org/public/igem/BBa_K112808/annotation1999021/range1999021/1
type: Range
persistent identity: range1999021
display id: range1999021
version: 1
top level: BBa_K112808
direction: direction/reverse
start: 1
end: 676
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation1999023
display id: annotation1999023
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999023/range1999023/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K112808/annotation1999023/range1999023/1
type: Range
persistent identity: range1999023
display id: range1999023
version: 1
top level: BBa_K112808
direction: direction/reverse
start: 685
end: 1198
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1999024
display id: annotation1999024
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999024/range1999024/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K112808/annotation1999024/range1999024/1
type: Range
persistent identity: range1999024
display id: range1999024
version: 1
top level: BBa_K112808
direction: direction/forward
start: 1207
end: 1286
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1999026
display id: annotation1999026
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999026/range1999026/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K112808/annotation1999026/range1999026/1
type: Range
persistent identity: range1999026
display id: range1999026
version: 1
top level: BBa_K112808
direction: direction/forward
start: 1295
end: 1335
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation1999030
display id: annotation1999030
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999030/range1999030/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K112808/annotation1999030/range1999030/1
type: Range
persistent identity: range1999030
display id: range1999030
version: 1
top level: BBa_K112808
direction: direction/reverse
start: 1344
end: 1378
orientation: inline

BBa_K112807
type: SequenceAnnotation
persistent identity: annotation1999032
display id: annotation1999032
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999032/range1999032/1
component: BBa_K112807

https://synbiohub.org/public/igem/BBa_K112808/annotation1999032/range1999032/1
type: Range
persistent identity: range1999032
display id: range1999032
version: 1
top level: BBa_K112808
direction: direction/reverse
start: 1387
end: 1697
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1999033
display id: annotation1999033
version: 1
top level: BBa_K112808
location: https://synbiohub.org/public/igem/BBa_K112808/annotation1999033/range1999033/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K112808/annotation1999033/range1999033/1
type: Range
persistent identity: range1999033
display id: range1999033
version: 1
top level: BBa_K112808
direction: direction/forward
start: 1706
end: 1785
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_K112805
type: ComponentDefinition
persistent identity: BBa K112805
display id: BBa_K112805
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 holin]
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=160221&to=160877&view=gbwithparts
owned by: james
owned by: myers
mutable description: Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer.
discontinued: false
dominant: true
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Making composite part of rbs.holin is problematic unless antiholin is expressed in bacteria together. Antiholin binds holin and inhibits the formation of holin.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 holin
sequence: https://synbiohub.org/public/igem/BBa_K112805_sequence/1

T4 holin
type: SequenceAnnotation
persistent identity: annotation1999005
display id: annotation1999005
version: 1
top level: BBa_K112805
location: https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
type: Range
persistent identity: range1999005
display id: range1999005
version: 1
top level: BBa_K112805
direction: direction/forward
start: 17
end: 673
orientation: inline

BBa_K112806
type: ComponentDefinition
persistent identity: BBa K112806
display id: BBa_K112806
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 endolysin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: In stock
status: status/Available
bookmark: false
mutable notes: High expression of lysozyme only is toxic to bacteria.
star: false
creator: Jin Huh
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K112806_sequence/1

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation1999018
display id: annotation1999018
version: 1
top level: BBa_K112806
location: https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
type: Range
persistent identity: range1999018
display id: range1999018
version: 1
top level: BBa_K112806
direction: direction/forward
start: 17
end: 511
orientation: inline

BBa_K112807
type: ComponentDefinition
persistent identity: BBa K112807
display id: BBa_K112807
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 antiholin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=59202&to=59495&view=gbwithparts
owned by: james
owned by: myers
mutable description: Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria.
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Higher constitutive expression of antiholin compared to the expression of holin is required for the stable lysis device.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 antiholin
sequence: https://synbiohub.org/public/igem/BBa_K112807_sequence/1

T4 antiholin
type: SequenceAnnotation
persistent identity: annotation1999019
display id: annotation1999019
version: 1
top level: BBa_K112807
location: https://synbiohub.org/public/igem/BBa_K112807/annotation1999019/range1999019/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112807/annotation1999019/range1999019/1
type: Range
persistent identity: range1999019
display id: range1999019
version: 1
top level: BBa_K112807
direction: direction/forward
start: 15
end: 308
orientation: inline

https://synbiohub.org/public/igem/BBa_S05032_sequence/1
type: Sequence
persistent identity: BBa S05032 sequence
display id: BBa_S05032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgagcgttgtataagcttttatgtctttctatatcaacttttaatagaaatataaagtaatataaatgtttttataataaattatgtgagatatatttttttgtccgtactggtatagatttgacgattaagtcttaaataagttataatctcaattgcgtaatttcttaaatacagaaataacaactacattggtagactgattaaaaagtgtacttgatgaactgttataaaccttaaaaaaataaaaataatagtttgggggatgttaaagatgtataaaaaatatggagattgttttaaaaagttgcgaaaccaaaagaatttagggttatcatactttagtaaacttggaatagaccgttcaaatatatcaagatttgaacatggaaaatgtatgatgagttttgagcgtatagatttgatgttagaagaaatgcaagttccgttatctgagtacgaattgattgtaaataattttatgccgaatttccaagaattttttatattagaattggaaaaagctgaatttagccaaaatcgagataaaataaaagagttgtattctgaggtcaaagaaacggggaatcatttactgacggttaccgtgaaaacgaagcttgggaatataagtcagacagaagttaaagaaattgaagcttatctttgcaatattgaagagtggggatattttgaacttactttattttattttgtatctgattatctcaatgtcaatcaattagaattgctgctttttaattttgataaaagatgtgaaaattactgtagagtcttaaaatatagaaggagactattgcaaatagcctataaaagtgttgcgatatacgcggctaaaggagaaagaaaaaaagccgaaaatattttagaaatgactaaaaaatatcgaactgtgggagtcgatttatattcagaagtattaagacatcttgctagagctatcattatttttaattttgaaaatgcagagattggggaagaaaaaataaattatgctcttgagattttggaagaatttggaggaaagaagataaaagaattttatcagaataaaatggaaaagtatttgaaaaggtcaatttagtctcttttgagctgttgctttaaagcaacagctcaaaagagattttctttattccagagcatatactagagggtgaagataggttgtctgaagcattataacttgtcttttaaaaaattcaatcataaatataaggaggtatgatgtactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K559011_sequence/1
type: Sequence
persistent identity: BBa K559011 sequence
display id: BBa_K559011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgagcgttgtataagcttttatgtctttctatatcaacttttaatagaaatataaagtaatataaatgtttttataataaattatgtgagatatatttttttgtccgtactggtatagatttgacgattaagtcttaaataagttataatctcaattgcgtaatttcttaaatacagaaataacaactacattggtagactgattaaaaagtgtacttgatgaactgttataaaccttaaaaaaataaaaataatagtttgggggatgttaaagatgtataaaaaatatggagattgttttaaaaagttgcgaaaccaaaagaatttagggttatcatactttagtaaacttggaatagaccgttcaaatatatcaagatttgaacatggaaaatgtatgatgagttttgagcgtatagatttgatgttagaagaaatgcaagttccgttatctgagtacgaattgattgtaaataattttatgccgaatttccaagaattttttatattagaattggaaaaagctgaatttagccaaaatcgagataaaataaaagagttgtattctgaggtcaaagaaacggggaatcatttactgacggttaccgtgaaaacgaagcttgggaatataagtcagacagaagttaaagaaattgaagcttatctttgcaatattgaagagtggggatattttgaacttactttattttattttgtatctgattatctcaatgtcaatcaattagaattgctgctttttaattttgataaaagatgtgaaaattactgtagagtcttaaaatatagaaggagactattgcaaatagcctataaaagtgttgcgatatacgcggctaaaggagaaagaaaaaaagccgaaaatattttagaaatgactaaaaaatatcgaactgtgggagtcgatttatattcagaagtattaagacatcttgctagagctatcattatttttaattttgaaaatgcagagattggggaagaaaaaataaattatgctcttgagattttggaagaatttggaggaaagaagataaaagaattttatcagaataaaatggaaaagtatttgaaaaggtcaatttagtctcttttgagctgttgctttaaagcaacagctcaaaagagattttctttattccagagcatatactagagggtgaagataggttgtctgaagcattataacttgtcttttaaaaaattcaatcataaatataaggaggtatgatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112808_sequence/1
type: Sequence
persistent identity: BBa K112808 sequence
display id: BBa_K112808_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23116_sequence/1
type: Sequence
persistent identity: BBa J23116 sequence
display id: BBa_J23116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112805_sequence/1
type: Sequence
persistent identity: BBa K112805 sequence
display id: BBa_K112805_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112806_sequence/1
type: Sequence
persistent identity: BBa K112806 sequence
display id: BBa_K112806_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112807_sequence/1
type: Sequence
persistent identity: BBa K112807 sequence
display id: BBa_K112807_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z