LOCUS BBa_S05035 5557 bp DNA linear 31-Oct-2024 DEFINITION S05034:K524003 ACCESSION BBa_S05035 VERSION BBa_S05035.1 FEATURES Location/Qualifiers CDS 2..184 /label=orf60a CDS CDS 373..1323 /label=repA101-ts misc_feature 1492..1574 /label=AT Rich region_oriR101 promoter 1329..1354 /label=Promoter region of repA101ts misc_feature 1391..1391 /label=point mutation, substitute of C as G to remove SpeI site misc_feature 1721..2067 /label=par region misc_feature 1..372 /label=regions not necessary for origin function misc_feature 1..2067 /label=BBa_K524000 promoter 3155..3285 /label=Pbad promoter 3118..3189 /label=PC misc_feature 2076..3154 /label=AraC promoter 2076..3285 /label=BBa_I0500 RBS 3294..3308 /label=RBS-1\Strong RBS 3301..3305 /label=RBS misc_feature 3294..3308 /label=BBa_B0030 RBS 3294..3308 /label=BBa_B0030 CDS 3315..4351 /label=BBa_J61047 misc_feature 2076..4351 /label=BBa_I718008 misc_feature 1..4351 /label=BBa_S05034 misc_feature 4360..4401 /label=sigma 70 promoter 4402..4459 /label=nadEp CDS 4558..5385 /label=NAD+ synthtase stem_loop 5429..5508 /label=B0010 stem_loop 5517..5557 /label=B0012 misc_feature 4547..4560 /label=post-transcriptional regulation by CyaR misc_feature 4360..5557 /label=BBa_K524003 ORIGIN 1 gatgacgcat cctcacgata atatccgggt aggcgcaatc actttcgtct actccgttac 61 aaagcgaggc tgggtatttc ccggcctttc tgttatccga aatccactga aagcacagcg 121 gctggctgag gagataaata ataaacgagg ggctgtatgc acaaagcatc ttctgttgag 181 ttaagaacga gtatcgagat ggcacatagc cttgctcaaa ttggaatcag gtttgtgcca 241 ataccagtag aaacagacga agaatccatg ggtatggaca gttttccctt tgatatgtaa 301 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga 361 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg 421 tttttgcgtg agccatgaga acgaaccatt gagatcatac ttactttgca tgtcactcaa 481 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt 541 tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt 601 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac 661 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct 721 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc 781 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt 841 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga 901 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg 961 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt 1021 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac 1081 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact 1141 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt 1201 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg 1261 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga 1321 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca 1381 atgataatta gtagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt 1441 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt 1501 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat 1561 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta 1621 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg 1681 cttaagtagc accctcgcaa gctcggttgc ggccgcaatc gggcaaatcg ctgaatattc 1741 cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct 1801 gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt 1861 catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt 1921 atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct 1981 gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc 2041 acccagtaag gcagcggtat catcaactac tagagttatg acaacttgac ggctacatca 2101 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 2161 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 2221 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 2281 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 2341 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 2401 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 2461 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 2521 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 2581 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 2641 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 2701 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 2761 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 2821 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 2881 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 2941 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 3001 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 3061 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 3121 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 3181 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 3241 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagctacta gagattaaag 3301 aggagaaata ctagatgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg 3361 tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg 3421 cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt 3481 gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc 3541 tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa 3601 acatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg 3661 ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag 3721 cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc 3781 aggatatacg taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg 3841 aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc 3901 atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg 3961 gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata 4021 actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc 4081 tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta 4141 aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg 4201 cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga 4261 ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg 4321 tgcgcctgct ggaagatggc gattaagaat ttactagagc cgtcctcttg tttatcagcg 4381 tgttagataa gcctggaata cattgggcgc tttttcaagc ccgtgaacga aacggctccg 4441 ctttcagagg attcctgtat gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt 4501 tcagtagcaa cgggttagct ttaaggaagt tttgtctttt ctgtctggag gggttcaatg 4561 acattgcaac aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag 4621 gaaattcgtc gtagtgtcga ttttctgaaa agctacctgc aaacttatcc gttcattaaa 4681 tcactggtgc tcgggatcag cggcggtcag gactccacgc ttgccggaaa gctgtgccag 4741 atggcgatta atgagctgcg cctggaaacc ggcaacgaat cactgcaatt tattgccgta 4801 cgcctgccct atggtgttca ggccgacgaa caagattgcc aggatgccat tgcctttatt 4861 caaccggatc gcgtattaac cgttaatatc aagggcgcgg tattggccag cgaacaggca 4921 ttgcgggaag caggcattga actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag 4981 cggatgaaag cacaatatag cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat 5041 catgcagcag aagccattac cggattcttc actaaatatg gtgacggcgg tacggacatt 5101 aatccgctgt atcgtctcaa caaacgtcag ggtaaacagt tactggcggc attagcttgc 5161 ccggaacacc tttataagaa agcgccaacg gccgatctgg aagatgatcg cccttctctg 5221 ccagatgaag tggcactcgg cgtgacctat gacaatatcg acgactatct ggaagggaaa 5281 aacgtacctc aacaggtcgc cagaacaata gagaactggt atctgaaaac cgaacataaa 5341 cgccgtccgc caattaccgt tttcgatgat ttctggaaaa agtaataatt tgcacatatt 5401 ggattgtgcg aaaaagagta atactagacc aggcatcaaa taaaacgaaa ggctcagtcg 5461 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 5521 actggctcac cttcgggtgg gcctttctgc gtttata //