##gff-version 3
##sequence-region BBa_S05035 1 5557
BBa_S05035	.	engineered_region	4360	5557	.	+	0	ID=BBa_K524003;Name=BBa_K524003
BBa_S05035	.	stem_loop	5429	5508	.	+	0	ID=annotation2153281;Name=B0010;Parent=BBa_K524003
BBa_S05035	.	non_covalent_binding_site	4360	4401	.	+	0	ID=annotation2153245;Name=sigma 70;Parent=BBa_K524003
BBa_S05035	.	stem_loop	5517	5557	.	+	0	ID=annotation2153321;Name=B0012;Parent=BBa_K524003
BBa_S05035	.	promoter	4402	4459	.	+	0	ID=annotation2153246;Name=nadEp;Parent=BBa_K524003
BBa_S05035	.	CDS	4558	5385	.	+	0	ID=annotation2153248;Name=NAD+ synthtase;Parent=BBa_K524003
BBa_S05035	.	sequence_feature	4547	4560	.	+	0	ID=annotation2153332;Name=post-transcriptional regulation by CyaR;Parent=BBa_K524003
BBa_S05035	.	engineered_region	1	4351	.	+	0	ID=BBa_S05034;Name=BBa_S05034
BBa_S05035	.	engineered_region	2076	4351	.	+	0	ID=BBa_I718008;Name=BBa_I718008;Parent=BBa_S05034
BBa_S05035	.	ribosome_entry_site	3294	3308	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_I718008
BBa_S05035	.	engineered_region	3294	3308	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S05035	.	ribosome_entry_site	3301	3305	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S05035	.	ribosome_entry_site	3294	3308	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S05035	.	promoter	2076	3285	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_I718008
BBa_S05035	.	promoter	3118	3189	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_S05035	.	promoter	3155	3285	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_S05035	.	sequence_feature	2076	3154	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_S05035	.	CDS	3315	4351	.	+	0	ID=BBa_J61047;Name=BBa_J61047;Parent=BBa_I718008
BBa_S05035	.	sequence_feature	1	2067	.	+	0	ID=BBa_K524000;Name=BBa_K524000;Parent=BBa_S05034
BBa_S05035	.	sequence_feature	1	372	.	+	0	ID=annotation2371777;Name=regions not necessary for origin function;Parent=BBa_K524000
BBa_S05035	.	sequence_feature	1721	2067	.	+	0	ID=annotation2371776;Name=par region;Parent=BBa_K524000
BBa_S05035	.	promoter	1329	1354	.	+	0	ID=annotation2138825;Name=Promoter region of repA101ts;Parent=BBa_K524000
BBa_S05035	.	CDS	373	1323	.	+	0	ID=annotation2138823;Name=repA101-ts;Parent=BBa_K524000
BBa_S05035	.	sequence_alteration	1391	1391	.	+	0	ID=annotation2138826;Name=point mutation, substitute of C as G to remove SpeI site;Parent=BBa_K524000
BBa_S05035	.	CDS	2	184	.	+	0	ID=annotation2138822;Name=orf60a CDS;Parent=BBa_K524000
BBa_S05035	.	sequence_feature	1492	1574	.	+	0	ID=annotation2138824;Name=AT Rich region_oriR101;Parent=BBa_K524000
>BBa_S05035
gatgacgcatcctcacgataatatccgggtaggcgcaatcactttcgtctactccgttacaaagcgaggctgggtattt
cccggcctttctgttatccgaaatccactgaaagcacagcggctggctgaggagataaataataaacgaggggctgtat
gcacaaagcatcttctgttgagttaagaacgagtatcgagatggcacatagccttgctcaaattggaatcaggtttgtg
ccaataccagtagaaacagacgaagaatccatgggtatggacagttttccctttgatatgtaacggtgaacagttgttc
tacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagcca
gtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtc
actcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgtta
cgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggtta
cgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaattt
catattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagtta
ttttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagtt
cttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaa
tttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatag
tttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttg
ctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgt
ccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctcc
gttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggt
gattttaatcactataccaattgagatgggctagtcaatgataattagtagtccttttcctttgagttgtgggtatctg
taaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgtt
ttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagcc
ctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaa
cagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcggttgcggccgcaatcgggcaaatcgctgaatat
tccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggc
agtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagccc
gtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgc
cctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcag
cggtatcatcaactactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcg
ctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacgg
tggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgct
aatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatca
aaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgact
cgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttcccc
ttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgta
ttggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccatt
cgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaa
ttctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcgg
tcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccg
gcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattg
catcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcatt
ctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattg
attatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgcttttt
atcgcaactctctactgtttctccatacccgtttttttgggctagctactagagattaaagaggagaaatactagatgt
ccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgat
ggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggca
tggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgc
gcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgacc
aagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacag
gctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgta
atctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctc
acgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggca
cttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgt
tttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttt
tgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcc
cgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatg
taaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgatta
agaatttactagagccgtcctcttgtttatcagcgtgttagataagcctggaatacattgggcgctttttcaagcccgt
gaacgaaacggctccgctttcagaggattcctgtatgacgttttaaccaccattcagcccgctgtcgcttgtcgtttca
gtagcaacgggttagctttaaggaagttttgtcttttctgtctggaggggttcaatgacattgcaacaacaaataataa
aggcgctgggcgcaaaaccgcagattaatgctgaagaggaaattcgtcgtagtgtcgattttctgaaaagctacctgca
aacttatccgttcattaaatcactggtgctcgggatcagcggcggtcaggactccacgcttgccggaaagctgtgccag
atggcgattaatgagctgcgcctggaaaccggcaacgaatcactgcaatttattgccgtacgcctgccctatggtgttc
aggccgacgaacaagattgccaggatgccattgcctttattcaaccggatcgcgtattaaccgttaatatcaagggcgc
ggtattggccagcgaacaggcattgcgggaagcaggcattgaactgagcgattttgtccgtggcaatgaaaaagcgcgt
gagcggatgaaagcacaatatagcattgcgggtatgaccagcggtgtcgtggtgggcaccgatcatgcagcagaagcca
ttaccggattcttcactaaatatggtgacggcggtacggacattaatccgctgtatcgtctcaacaaacgtcagggtaa
acagttactggcggcattagcttgcccggaacacctttataagaaagcgccaacggccgatctggaagatgatcgccct
tctctgccagatgaagtggcactcggcgtgacctatgacaatatcgacgactatctggaagggaaaaacgtacctcaac
aggtcgccagaacaatagagaactggtatctgaaaaccgaacataaacgccgtccgccaattaccgttttcgatgattt
ctggaaaaagtaataatttgcacatattggattgtgcgaaaaagagtaatactagaccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata