BBa_S05035
type: ComponentDefinition
persistent identity: BBa S05035
display id: BBa_S05035
version: 1
was generated by: iGEM to SBOL conversion
description: S05034:K524003
created: 2012-08-23T11:00:00Z
modified: 2015-05-08T01:14:48Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 8758
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: HO Yuan Heng Trevor
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S05034
component: BBa_K524003
sequence annotation: BBa_S05034
sequence annotation: BBa_K524003
sequence: https://synbiohub.org/public/igem/BBa_S05035_sequence/1

BBa_S05034
type: Component
persistent identity: component2373471
display id: component2373471
version: 1
top level: BBa_S05035
definition: BBa_S05034
access: public

BBa_K524003
type: Component
persistent identity: component2373478
display id: component2373478
version: 1
top level: BBa_S05035
definition: BBa_K524003
access: public

BBa_S05034
type: SequenceAnnotation
persistent identity: annotation2373471
display id: annotation2373471
version: 1
top level: BBa_S05035
location: https://synbiohub.org/public/igem/BBa_S05035/annotation2373471/range2373471/1
component: BBa_S05034

https://synbiohub.org/public/igem/BBa_S05035/annotation2373471/range2373471/1
type: Range
persistent identity: range2373471
display id: range2373471
version: 1
top level: BBa_S05035
direction: direction/reverse
start: 1
end: 4351
orientation: inline

BBa_K524003
type: SequenceAnnotation
persistent identity: annotation2373478
display id: annotation2373478
version: 1
top level: BBa_S05035
location: https://synbiohub.org/public/igem/BBa_S05035/annotation2373478/range2373478/1
component: BBa_K524003

https://synbiohub.org/public/igem/BBa_S05035/annotation2373478/range2373478/1
type: Range
persistent identity: range2373478
display id: range2373478
version: 1
top level: BBa_S05035
direction: direction/reverse
start: 4360
end: 5557
orientation: inline

BBa_K524003
type: ComponentDefinition
persistent identity: BBa K524003
display id: BBa_K524003
version: 1
was generated by: iGEM to SBOL conversion
description: nadE gene
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:34Z
mutable provenance: From genomic DNA of BL21(DE3)
owned by: james
owned by: myers
mutable description: (to be entered)
discontinued: false
dominant: false
experience: experience/None
group u list: _689_
m user id: 0
owner id: 8758
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: nil
star: false
creator: HO Yuan Heng Trevor
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: sigma 70
sequence annotation: nadEp
sequence annotation: post-transcriptional regulation by CyaR
sequence annotation: NAD+ synthtase
sequence annotation: B0010
sequence annotation: B0012
sequence: https://synbiohub.org/public/igem/BBa_K524003_sequence/1

sigma 70
type: SequenceAnnotation
persistent identity: annotation2153245
display id: annotation2153245
version: 1
top level: BBa_K524003
location: https://synbiohub.org/public/igem/BBa_K524003/annotation2153245/range2153245/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K524003/annotation2153245/range2153245/1
type: Range
persistent identity: range2153245
display id: range2153245
version: 1
top level: BBa_K524003
direction: direction/forward
start: 1
end: 42
orientation: inline

nadEp
type: SequenceAnnotation
persistent identity: annotation2153246
display id: annotation2153246
version: 1
top level: BBa_K524003
location: https://synbiohub.org/public/igem/BBa_K524003/annotation2153246/range2153246/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K524003/annotation2153246/range2153246/1
type: Range
persistent identity: range2153246
display id: range2153246
version: 1
top level: BBa_K524003
direction: direction/forward
start: 43
end: 100
orientation: inline

post-transcriptional regulation by CyaR
type: SequenceAnnotation
persistent identity: annotation2153332
display id: annotation2153332
version: 1
top level: BBa_K524003
location: https://synbiohub.org/public/igem/BBa_K524003/annotation2153332/range2153332/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K524003/annotation2153332/range2153332/1
type: Range
persistent identity: range2153332
display id: range2153332
version: 1
top level: BBa_K524003
direction: direction/forward
start: 188
end: 201
orientation: inline

NAD+ synthtase
type: SequenceAnnotation
persistent identity: annotation2153248
display id: annotation2153248
version: 1
top level: BBa_K524003
location: https://synbiohub.org/public/igem/BBa_K524003/annotation2153248/range2153248/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K524003/annotation2153248/range2153248/1
type: Range
persistent identity: range2153248
display id: range2153248
version: 1
top level: BBa_K524003
direction: direction/forward
start: 199
end: 1026
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation2153281
display id: annotation2153281
version: 1
top level: BBa_K524003
location: https://synbiohub.org/public/igem/BBa_K524003/annotation2153281/range2153281/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K524003/annotation2153281/range2153281/1
type: Range
persistent identity: range2153281
display id: range2153281
version: 1
top level: BBa_K524003
direction: direction/forward
start: 1070
end: 1149
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation2153321
display id: annotation2153321
version: 1
top level: BBa_K524003
location: https://synbiohub.org/public/igem/BBa_K524003/annotation2153321/range2153321/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K524003/annotation2153321/range2153321/1
type: Range
persistent identity: range2153321
display id: range2153321
version: 1
top level: BBa_K524003
direction: direction/forward
start: 1158
end: 1198
orientation: inline

BBa_S05034
type: ComponentDefinition
persistent identity: BBa S05034
display id: BBa_S05034
version: 1
was generated by: iGEM to SBOL conversion
description: K524000:I718008
created: 2012-08-23T11:00:00Z
modified: 2015-05-08T01:14:48Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 8758
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: HO Yuan Heng Trevor
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K524000
component: BBa_I718008
sequence annotation: BBa_K524000
sequence annotation: BBa_I718008
sequence: https://synbiohub.org/public/igem/BBa_S05034_sequence/1

BBa_K524000
type: Component
persistent identity: component2373209
display id: component2373209
version: 1
top level: BBa_S05034
definition: BBa_K524000
access: public

BBa_I718008
type: Component
persistent identity: component2373218
display id: component2373218
version: 1
top level: BBa_S05034
definition: BBa_I718008
access: public

BBa_K524000
type: SequenceAnnotation
persistent identity: annotation2373209
display id: annotation2373209
version: 1
top level: BBa_S05034
location: https://synbiohub.org/public/igem/BBa_S05034/annotation2373209/range2373209/1
component: BBa_K524000

https://synbiohub.org/public/igem/BBa_S05034/annotation2373209/range2373209/1
type: Range
persistent identity: range2373209
display id: range2373209
version: 1
top level: BBa_S05034
direction: direction/reverse
start: 1
end: 2067
orientation: inline

BBa_I718008
type: SequenceAnnotation
persistent identity: annotation2373218
display id: annotation2373218
version: 1
top level: BBa_S05034
location: https://synbiohub.org/public/igem/BBa_S05034/annotation2373218/range2373218/1
component: BBa_I718008

https://synbiohub.org/public/igem/BBa_S05034/annotation2373218/range2373218/1
type: Range
persistent identity: range2373218
display id: range2373218
version: 1
top level: BBa_S05034
direction: direction/reverse
start: 2076
end: 4351
orientation: inline

BBa_I718008
type: ComponentDefinition
persistent identity: BBa I718008
display id: BBa_I718008
version: 1
was generated by: iGEM to SBOL conversion
description: araC-pBad-rbs-cre
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: none
discontinued: false
dominant: false
experience: experience/Works
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none
star: true
creator: David Bikard
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_J61047
component: BBa_B0030
sequence annotation: BBa_I0500
sequence annotation: BBa_B0030
sequence annotation: BBa_J61047
sequence: https://synbiohub.org/public/igem/BBa_I718008_sequence/1

BBa_I0500
type: Component
persistent identity: component1956079
display id: component1956079
version: 1
top level: BBa_I718008
definition: pBad/araC
access: public

BBa_J61047
type: Component
persistent identity: component1956083
display id: component1956083
version: 1
top level: BBa_I718008
definition: Cre
access: public

BBa_B0030
type: Component
persistent identity: component1956081
display id: component1956081
version: 1
top level: BBa_I718008
definition: BBa_B0030
access: public

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1956079
display id: annotation1956079
version: 1
top level: BBa_I718008
location: https://synbiohub.org/public/igem/BBa_I718008/annotation1956079/range1956079/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I718008/annotation1956079/range1956079/1
type: Range
persistent identity: range1956079
display id: range1956079
version: 1
top level: BBa_I718008
direction: direction/reverse
start: 1
end: 1210
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956081
display id: annotation1956081
version: 1
top level: BBa_I718008
location: https://synbiohub.org/public/igem/BBa_I718008/annotation1956081/range1956081/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718008/annotation1956081/range1956081/1
type: Range
persistent identity: range1956081
display id: range1956081
version: 1
top level: BBa_I718008
direction: direction/forward
start: 1219
end: 1233
orientation: inline

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation1956083
display id: annotation1956083
version: 1
top level: BBa_I718008
location: https://synbiohub.org/public/igem/BBa_I718008/annotation1956083/range1956083/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_I718008/annotation1956083/range1956083/1
type: Range
persistent identity: range1956083
display id: range1956083
version: 1
top level: BBa_I718008
direction: direction/reverse
start: 1240
end: 2276
orientation: inline

BBa_K524000
type: ComponentDefinition
persistent identity: BBa K524000
display id: BBa_K524000
version: 1
was generated by: iGEM to SBOL conversion
description: Heat sensitive origin of replication (oriR101 & repA101-ts)
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:34Z
mutable provenance: From the plasmid pKD46 maintained inside the E.coli strain BW25113, courtesy of E.coli Genetic Resources at Yale CGSC, The Coli Genetic Stock Center.
owned by: james
owned by: myers
mutable description: OriR101 is an low copy origin of replication that is initiated by the protein repA101. repA101-ts, a mutated form of repA101 would denature at temperatures above 37??C. Hence plasmids using this construct to drive replication can only initiate replication when cultured below 37??C (recommended <30??C). Production of repA101-ts is drived by an unknown constitutive promoter included in the construct.
discontinued: false
dominant: false
experience: experience/Works
group u list: _689_
m user id: 0
owner id: 8750
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: To make this part compatible with Biobrick standard assembly protocols, overlapping PCR was used to mutate a SpeI cut site originally present inside the construct to render it nonfunctional.
star: false
creator: HO Yuan Heng Trevor
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: regions not necessary for origin function
sequence annotation: orf60a CDS
sequence annotation: repA101-ts
sequence annotation: Promoter region of repA101ts
sequence annotation: point mutation, substitute of C as G to remove SpeI site
sequence annotation: AT Rich region_oriR101
sequence annotation: par region
sequence: https://synbiohub.org/public/igem/BBa_K524000_sequence/1

regions not necessary for origin function
type: SequenceAnnotation
persistent identity: annotation2371777
display id: annotation2371777
version: 1
top level: BBa_K524000
location: https://synbiohub.org/public/igem/BBa_K524000/annotation2371777/range2371777/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K524000/annotation2371777/range2371777/1
type: Range
persistent identity: range2371777
display id: range2371777
version: 1
top level: BBa_K524000
direction: direction/forward
start: 1
end: 372
orientation: inline

orf60a CDS
type: SequenceAnnotation
persistent identity: annotation2138822
display id: annotation2138822
version: 1
top level: BBa_K524000
location: https://synbiohub.org/public/igem/BBa_K524000/annotation2138822/range2138822/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K524000/annotation2138822/range2138822/1
type: Range
persistent identity: range2138822
display id: range2138822
version: 1
top level: BBa_K524000
direction: direction/forward
start: 2
end: 184
orientation: inline

repA101-ts
type: SequenceAnnotation
persistent identity: annotation2138823
display id: annotation2138823
version: 1
top level: BBa_K524000
location: https://synbiohub.org/public/igem/BBa_K524000/annotation2138823/range2138823/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K524000/annotation2138823/range2138823/1
type: Range
persistent identity: range2138823
display id: range2138823
version: 1
top level: BBa_K524000
direction: direction/reverse
start: 373
end: 1323
orientation: inline

Promoter region of repA101ts
type: SequenceAnnotation
persistent identity: annotation2138825
display id: annotation2138825
version: 1
top level: BBa_K524000
location: https://synbiohub.org/public/igem/BBa_K524000/annotation2138825/range2138825/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K524000/annotation2138825/range2138825/1
type: Range
persistent identity: range2138825
display id: range2138825
version: 1
top level: BBa_K524000
direction: direction/reverse
start: 1329
end: 1354
orientation: inline

point mutation, substitute of C as G to remove SpeI site
type: SequenceAnnotation
persistent identity: annotation2138826
display id: annotation2138826
version: 1
top level: BBa_K524000
location: https://synbiohub.org/public/igem/BBa_K524000/annotation2138826/range2138826/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K524000/annotation2138826/range2138826/1
type: Range
persistent identity: range2138826
display id: range2138826
version: 1
top level: BBa_K524000
direction: direction/forward
start: 1391
end: 1391
orientation: inline

AT Rich region_oriR101
type: SequenceAnnotation
persistent identity: annotation2138824
display id: annotation2138824
version: 1
top level: BBa_K524000
location: https://synbiohub.org/public/igem/BBa_K524000/annotation2138824/range2138824/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K524000/annotation2138824/range2138824/1
type: Range
persistent identity: range2138824
display id: range2138824
version: 1
top level: BBa_K524000
direction: direction/forward
start: 1492
end: 1574
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation2371776
display id: annotation2371776
version: 1
top level: BBa_K524000
location: https://synbiohub.org/public/igem/BBa_K524000/annotation2371776/range2371776/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K524000/annotation2371776/range2371776/1
type: Range
persistent identity: range2371776
display id: range2371776
version: 1
top level: BBa_K524000
direction: direction/reverse
start: 1721
end: 2067
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

Cre
type: ComponentDefinition
persistent identity: BBa J61047
display id: BBa_J61047
version: 1
was generated by: iGEM to SBOL conversion
description: Cre DNA recombinase
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J61047_sequence/1

https://synbiohub.org/public/igem/BBa_S05035_sequence/1
type: Sequence
persistent identity: BBa S05035 sequence
display id: BBa_S05035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatgacgcatcctcacgataatatccgggtaggcgcaatcactttcgtctactccgttacaaagcgaggctgggtatttcccggcctttctgttatccgaaatccactgaaagcacagcggctggctgaggagataaataataaacgaggggctgtatgcacaaagcatcttctgttgagttaagaacgagtatcgagatggcacatagccttgctcaaattggaatcaggtttgtgccaataccagtagaaacagacgaagaatccatgggtatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattagtagtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcggttgcggccgcaatcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccact…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K524003_sequence/1
type: Sequence
persistent identity: BBa K524003 sequence
display id: BBa_K524003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccgtcctcttgtttatcagcgtgttagataagcctggaatacattgggcgctttttcaagcccgtgaacgaaacggctccgctttcagaggattcctgtatgacgttttaaccaccattcagcccgctgtcgcttgtcgtttcagtagcaacgggttagctttaaggaagttttgtcttttctgtctggaggggttcaatgacattgcaacaacaaataataaaggcgctgggcgcaaaaccgcagattaatgctgaagaggaaattcgtcgtagtgtcgattttctgaaaagctacctgcaaacttatccgttcattaaatcactggtgctcgggatcagcggcggtcaggactccacgcttgccggaaagctgtgccagatggcgattaatgagctgcgcctggaaaccggcaacgaatcactgcaatttattgccgtacgcctgccctatggtgttcaggccgacgaacaagattgccaggatgccattgcctttattcaaccggatcgcgtattaaccgttaatatcaagggcgcggtattggccagcgaacaggcattgcgggaagcaggcattgaactgagcgattttgtccgtggcaatgaaaaagcgcgtgagcggatgaaagcacaatatagcattgcgggtatgaccagcggtgtcgtggtgggcaccgatcatgcagcagaagccattaccggattcttcactaaatatggtgacggcggtacggacattaatccgctgtatcgtctcaacaaacgtcagggtaaacagttactggcggcattagcttgcccggaacacctttataagaaagcgccaacggccgatctggaagatgatcgcccttctctgccagatgaagtggcactcggcgtgacctatgacaatatcgacgactatctggaagggaaaaacgtacctcaacaggtcgccagaacaatagagaactggtatctgaaaaccgaacataaacgccgtccgccaattaccgttttcgatgatttctggaaaaagtaataatttgcacatattggattgtgcgaaaaagagtaatactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05034_sequence/1
type: Sequence
persistent identity: BBa S05034 sequence
display id: BBa_S05034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatgacgcatcctcacgataatatccgggtaggcgcaatcactttcgtctactccgttacaaagcgaggctgggtatttcccggcctttctgttatccgaaatccactgaaagcacagcggctggctgaggagataaataataaacgaggggctgtatgcacaaagcatcttctgttgagttaagaacgagtatcgagatggcacatagccttgctcaaattggaatcaggtttgtgccaataccagtagaaacagacgaagaatccatgggtatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattagtagtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcggttgcggccgcaatcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccact…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I718008_sequence/1
type: Sequence
persistent identity: BBa I718008 sequence
display id: BBa_I718008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagattaaagaggagaaatactagatgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K524000_sequence/1
type: Sequence
persistent identity: BBa K524000 sequence
display id: BBa_K524000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatgacgcatcctcacgataatatccgggtaggcgcaatcactttcgtctactccgttacaaagcgaggctgggtatttcccggcctttctgttatccgaaatccactgaaagcacagcggctggctgaggagataaataataaacgaggggctgtatgcacaaagcatcttctgttgagttaagaacgagtatcgagatggcacatagccttgctcaaattggaatcaggtttgtgccaataccagtagaaacagacgaagaatccatgggtatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattagtagtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcggttgcggccgcaatcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccact…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61047_sequence/1
type: Sequence
persistent identity: BBa J61047 sequence
display id: BBa_J61047_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattaagaatt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z