LOCUS       BBa_S05036              2275 bp    DNA     linear       23-Dec-2024
DEFINITION  J04450:K524003
ACCESSION   BBa_S05036
VERSION     BBa_S05036.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI
     misc_feature    173..173
                     /label=start
     misc_feature    209..220
                     /label=BBa_B0034
     misc_feature    213..216
                     /label=conserved
     misc_feature    227..907
                     /label=BBa_E1010
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=barcode
     misc_feature    941..1020
                     /label=BBa_B0010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    1029..1069
                     /label=BBa_B0012
     stem_loop       1036..1055
                     /label=T7 TE
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1062..1062
                     /label=stop
     misc_feature    1..1069
                     /label=BBa_J04450
     misc_feature    1078..1119
                     /label=sigma 70
     promoter        1120..1177
                     /label=nadEp
     misc_feature    1265..1278
                     /label=post-transcriptional regulation by CyaR
     CDS             1276..2103
                     /label=NAD+ synthtase
     stem_loop       2147..2226
                     /label=B0010
     stem_loop       2235..2275
                     /label=B0012
     misc_feature    1078..2275
                     /label=BBa_K524003
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagccg
     1081 tcctcttgtt tatcagcgtg ttagataagc ctggaataca ttgggcgctt tttcaagccc
     1141 gtgaacgaaa cggctccgct ttcagaggat tcctgtatga cgttttaacc accattcagc
     1201 ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt aaggaagttt tgtcttttct
     1261 gtctggaggg gttcaatgac attgcaacaa caaataataa aggcgctggg cgcaaaaccg
     1321 cagattaatg ctgaagagga aattcgtcgt agtgtcgatt ttctgaaaag ctacctgcaa
     1381 acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga ctccacgctt
     1441 gccggaaagc tgtgccagat ggcgattaat gagctgcgcc tggaaaccgg caacgaatca
     1501 ctgcaattta ttgccgtacg cctgccctat ggtgttcagg ccgacgaaca agattgccag
     1561 gatgccattg cctttattca accggatcgc gtattaaccg ttaatatcaa gggcgcggta
     1621 ttggccagcg aacaggcatt gcgggaagca ggcattgaac tgagcgattt tgtccgtggc
     1681 aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat gaccagcggt
     1741 gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg gattcttcac taaatatggt
     1801 gacggcggta cggacattaa tccgctgtat cgtctcaaca aacgtcaggg taaacagtta
     1861 ctggcggcat tagcttgccc ggaacacctt tataagaaag cgccaacggc cgatctggaa
     1921 gatgatcgcc cttctctgcc agatgaagtg gcactcggcg tgacctatga caatatcgac
     1981 gactatctgg aagggaaaaa cgtacctcaa caggtcgcca gaacaataga gaactggtat
     2041 ctgaaaaccg aacataaacg ccgtccgcca attaccgttt tcgatgattt ctggaaaaag
     2101 taataatttg cacatattgg attgtgcgaa aaagagtaat actagaccag gcatcaaata
     2161 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2221 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//