LOCUS       BBa_S05037              2275 bp    DNA     linear       09-Dec-2025
DEFINITION  K524003:J04450
ACCESSION   BBa_S05037
VERSION     BBa_S05037.1  
FEATURES             Location/Qualifiers
     misc_feature    1..42
                     /label=sigma 70
     promoter        43..100
                     /label=nadEp
     CDS             199..1026
                     /label=NAD+ synthtase
     stem_loop       1070..1149
                     /label=B0010
     stem_loop       1158..1198
                     /label=B0012
     misc_feature    188..201
                     /label=post-transcriptional regulation by CyaR
     misc_feature    1..1198
                     /label=BBa_K524003
     CDS             1207..1294
                     /label=end of LacI coding region (inactive)
     misc_feature    1207..1406
                     /label=BBa_R0010
     misc_feature    1295..1332
                     /label=CAP binding site
     promoter        1343..1348
                     /label=-35
     promoter        1367..1372
                     /label=-10
     misc_feature    1372..1406
                     /label=LacI binding site
     misc_feature    1379..1379
                     /label=start
     promoter        1207..1406
                     /label=BBa_R0010
     misc_feature    1419..1422
                     /label=conserved
     RBS             1415..1426
                     /label=BBa_B0034
     CDS             1433..2107
                     /label=mrfp1
     misc_feature    2114..2138
                     /label=Help:Barcodes
     CDS             1433..2113
                     /label=BBa_E1010
     stem_loop       2158..2201
                     /label=stem_loop
     misc_feature    2147..2226
                     /label=BBa_B0010
     terminator      2147..2226
                     /label=BBa_B0010
     stem_loop       2242..2261
                     /label=T7 TE
     misc_feature    2268..2268
                     /label=stop
     polyA_site      2262..2275
                     /label=polya
     misc_feature    2235..2275
                     /label=BBa_B0012
     terminator      2235..2275
                     /label=BBa_B0012
     misc_feature    1207..2275
                     /label=BBa_J04450
ORIGIN
        1 ccgtcctctt gtttatcagc gtgttagata agcctggaat acattgggcg ctttttcaag
       61 cccgtgaacg aaacggctcc gctttcagag gattcctgta tgacgtttta accaccattc
      121 agcccgctgt cgcttgtcgt ttcagtagca acgggttagc tttaaggaag ttttgtcttt
      181 tctgtctgga ggggttcaat gacattgcaa caacaaataa taaaggcgct gggcgcaaaa
      241 ccgcagatta atgctgaaga ggaaattcgt cgtagtgtcg attttctgaa aagctacctg
      301 caaacttatc cgttcattaa atcactggtg ctcgggatca gcggcggtca ggactccacg
      361 cttgccggaa agctgtgcca gatggcgatt aatgagctgc gcctggaaac cggcaacgaa
      421 tcactgcaat ttattgccgt acgcctgccc tatggtgttc aggccgacga acaagattgc
      481 caggatgcca ttgcctttat tcaaccggat cgcgtattaa ccgttaatat caagggcgcg
      541 gtattggcca gcgaacaggc attgcgggaa gcaggcattg aactgagcga ttttgtccgt
      601 ggcaatgaaa aagcgcgtga gcggatgaaa gcacaatata gcattgcggg tatgaccagc
      661 ggtgtcgtgg tgggcaccga tcatgcagca gaagccatta ccggattctt cactaaatat
      721 ggtgacggcg gtacggacat taatccgctg tatcgtctca acaaacgtca gggtaaacag
      781 ttactggcgg cattagcttg cccggaacac ctttataaga aagcgccaac ggccgatctg
      841 gaagatgatc gcccttctct gccagatgaa gtggcactcg gcgtgaccta tgacaatatc
      901 gacgactatc tggaagggaa aaacgtacct caacaggtcg ccagaacaat agagaactgg
      961 tatctgaaaa ccgaacataa acgccgtccg ccaattaccg ttttcgatga tttctggaaa
     1021 aagtaataat ttgcacatat tggattgtgc gaaaaagagt aatactagac caggcatcaa
     1081 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1141 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     1201 ctagagcaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1261 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     1321 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     1381 ttgtgagcgg ataacaattt cacacatact agagaaagag gagaaatact agatggcttc
     1441 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
     1501 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
     1561 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
     1621 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
     1681 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
     1741 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
     1801 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
     1861 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
     1921 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
     1981 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
     2041 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
     2101 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata
     2161 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2221 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//