##gff-version 3
##sequence-region BBa_S05037 1 2275
BBa_S05037	.	engineered_region	1207	2275	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_S05037	.	ribosome_entry_site	1415	1426	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_S05037	.	conserved_region	1419	1422	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05037	.	promoter	1207	1406	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_S05037	.	CDS	1207	1294	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S05037	.	promoter	1367	1372	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S05037	.	non_covalent_binding_site	1372	1406	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S05037	.	engineered_region	1207	1406	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S05037	.	promoter	1343	1348	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S05037	.	non_covalent_binding_site	1295	1332	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S05037	.	start_codon	1379	1379	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S05037	.	CDS	1433	2113	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_S05037	.	CDS	1433	2107	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S05037	.	engineered_tag	2114	2138	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S05037	.	terminator	2147	2226	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_S05037	.	stem_loop	2158	2201	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05037	.	engineered_region	2147	2226	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05037	.	terminator	2235	2275	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_S05037	.	stem_loop	2242	2261	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05037	.	polyA_site	2262	2275	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05037	.	stop_codon	2268	2268	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05037	.	engineered_region	2235	2275	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S05037	.	engineered_region	1	1198	.	+	0	ID=BBa_K524003;Name=BBa_K524003
BBa_S05037	.	sequence_feature	188	201	.	+	0	ID=annotation2153332;Name=post-transcriptional regulation by CyaR;Parent=BBa_K524003
BBa_S05037	.	non_covalent_binding_site	1	42	.	+	0	ID=annotation2153245;Name=sigma 70;Parent=BBa_K524003
BBa_S05037	.	stem_loop	1070	1149	.	+	0	ID=annotation2153281;Name=B0010;Parent=BBa_K524003
BBa_S05037	.	promoter	43	100	.	+	0	ID=annotation2153246;Name=nadEp;Parent=BBa_K524003
BBa_S05037	.	stem_loop	1158	1198	.	+	0	ID=annotation2153321;Name=B0012;Parent=BBa_K524003
BBa_S05037	.	CDS	199	1026	.	+	0	ID=annotation2153248;Name=NAD+ synthtase;Parent=BBa_K524003
>BBa_S05037
ccgtcctcttgtttatcagcgtgttagataagcctggaatacattgggcgctttttcaagcccgtgaacgaaacggctc
cgctttcagaggattcctgtatgacgttttaaccaccattcagcccgctgtcgcttgtcgtttcagtagcaacgggtta
gctttaaggaagttttgtcttttctgtctggaggggttcaatgacattgcaacaacaaataataaaggcgctgggcgca
aaaccgcagattaatgctgaagaggaaattcgtcgtagtgtcgattttctgaaaagctacctgcaaacttatccgttca
ttaaatcactggtgctcgggatcagcggcggtcaggactccacgcttgccggaaagctgtgccagatggcgattaatga
gctgcgcctggaaaccggcaacgaatcactgcaatttattgccgtacgcctgccctatggtgttcaggccgacgaacaa
gattgccaggatgccattgcctttattcaaccggatcgcgtattaaccgttaatatcaagggcgcggtattggccagcg
aacaggcattgcgggaagcaggcattgaactgagcgattttgtccgtggcaatgaaaaagcgcgtgagcggatgaaagc
acaatatagcattgcgggtatgaccagcggtgtcgtggtgggcaccgatcatgcagcagaagccattaccggattcttc
actaaatatggtgacggcggtacggacattaatccgctgtatcgtctcaacaaacgtcagggtaaacagttactggcgg
cattagcttgcccggaacacctttataagaaagcgccaacggccgatctggaagatgatcgcccttctctgccagatga
agtggcactcggcgtgacctatgacaatatcgacgactatctggaagggaaaaacgtacctcaacaggtcgccagaaca
atagagaactggtatctgaaaaccgaacataaacgccgtccgccaattaccgttttcgatgatttctggaaaaagtaat
aatttgcacatattggattgtgcgaaaaagagtaatactagaccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacga
caggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggct
ttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagagga
gaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaac
ggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttacca
aaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacaccc
ggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggt
ggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaact
tcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacgg
tgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctac
atggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagact
acaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtag
atcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata