LOCUS       BBa_S05038              1712 bp    DNA     linear       25-Nov-2024
DEFINITION  J04450:K524200
ACCESSION   BBa_S05038
VERSION     BBa_S05038.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
     misc_feature    1..1069
                     /label=BBa_J04450
     stem_loop       1149..1174
                     /label=Terminator Loop
     CDS             1213..1431
                     /label=infA CDS
     promoter        1680..1685
                     /label=infAp1 -10
     promoter        1471..1476
                     /label=infAp2
     promoter        1707..1712
                     /label=infAp1 -35
     promoter        1497..1502
                     /label=infAp2 -35
     misc_feature    1078..1712
                     /label=BBa_K524200
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtgt
     1081 gcagtatatc tacatcgaga caagttacgg acttatactt ccaaagtact tcatacatat
     1141 cacaaaataa aaaggccggt taaaccgacc ttttactcgt tctttctctt cgcccatcag
     1201 gcggtaaaac aatcagcgac tacggaagac aatgcggcct ttgctcaggt cgtacggggt
     1261 cagttcaaca gtcactttgt cgcccgtcag gatgcggatg tagtttttgc gcattttacc
     1321 ggagatgtgt gcagtaacca cgtgaccgtt ttctaactct acgcggaaca tggtattagg
     1381 caacgtttca agaacggtac cttgcatttc aatattgtct tctttggcca tctaatcctc
     1441 tggggtatca ctaccgtaat ttgaaccggc aagataatgc cgaagttctg taaataagta
     1501 aagatttgcg cgctaaatcg caacaaacag gttcggcaca ttactccgaa aacacacggc
     1561 taagccgcac caaaagcgca acgtataagg gagcggtgag ataaacgatg ggcgttacct
     1621 gacgcgaaaa attccttatc ggcagcgggg taatgagcgt aaccaactct gcgaccgcaa
     1681 ttataacact ctggggagaa atgtgccgaa aa
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