LOCUS       BBa_S05039              3293 bp    DNA     linear       22-Nov-2024
DEFINITION  R0011:I732019
ACCESSION   BBa_S05039
VERSION     BBa_S05039.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    3105..3105
                     /label=C->T
     CDS             82..3156
                     /label=lacZ
     misc_feature    82..84
                     /label=START
     misc_feature    3154..3156
                     /label=STOP
     CDS             82..3156
                     /label=BBa_I732005
     stem_loop       3176..3219
                     /label=stem_loop
     misc_feature    3165..3244
                     /label=BBa_B0010
     terminator      3165..3244
                     /label=BBa_B0010
     stem_loop       3260..3279
                     /label=T7 TE
     misc_feature    3286..3286
                     /label=stop
     polyA_site      3280..3293
                     /label=polya
     misc_feature    3253..3293
                     /label=BBa_B0012
     terminator      3253..3293
                     /label=BBa_B0012
     misc_feature    64..3293
                     /label=BBa_I732019
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaccatg attacggatt cactggccgt cgttttacaa
      121 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      181 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      241 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
      301 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
      361 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
      421 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
      481 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg
      541 tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac
      601 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt
      661 gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc
      721 tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat
      781 gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac
      841 tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg
      901 cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt
      961 ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg
     1021 gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc
     1081 cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga
     1141 ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg
     1201 gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat
     1261 ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa
     1321 gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg
     1381 ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt
     1441 gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat
     1501 cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac
     1561 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc
     1621 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg
     1681 ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac
     1741 tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat
     1801 cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt
     1861 ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg
     1921 ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc
     1981 gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg
     2041 cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc
     2101 gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg
     2161 caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg
     2221 cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc
     2281 gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt
     2341 aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat
     2401 aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac
     2461 attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg
     2521 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat
     2581 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc
     2641 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg
     2701 gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca
     2761 gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc
     2821 gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg
     2881 agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc
     2941 ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat
     3001 cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg
     3061 attggtggcg acgactcctg gagcccgtca gtatcggcgg aatttcagct gagcgccggt
     3121 cgctaccatt accagttggt ctggtgtcaa aaataatact agagccaggc atcaaataaa
     3181 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     3241 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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