BBa_S05048 1 BBa_S05048 B0034:K748000 2012-09-14T11:00:00Z 2015-05-08T01:14:48Z Released HQ 2013 false false _9_ 0 8564 9 In stock false false Lei Qiao component2183411 1 BBa_B0034 component2183412 1 BBa_K748000 annotation2183411 1 BBa_B0034 range2183411 1 1 12 annotation2183412 1 BBa_K748000 range2183412 1 19 648 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K748000 1 BBa_K748000 AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system. 2012-09-14T11:00:00Z 2015-05-08T01:13:12Z Staphylococcus aureus genomic sequence Staphylococcus aureus AgrA, the transcriptional component of a quorum sensing system and global regulator of virulence that up-regulates secreted virulence factors and down-regulates cell wall-associated proteins, can bind in both the P2 and P3 promoter regions of the agr locus. The composite of AgrC and AIP then leads to phosphorylation of AgrA. AgrA in its phosphorylated sate activates transcription from both P2 and P3, leading to the production of GFP and 3OC6HSL. Thus we can detect the presence of S.aureus expediently by observing the expression of GFP. false false _999_ 0 8564 9 It's complicated false As a AIP sensor system, agrA should work with agrC. false Lei Qiao BBa_B0034_sequence 1 aaagaggagaaa BBa_S05048_sequence 1 aaagaggagaaatactagatggaaattgcattagcaacagataatccatatgaagttttagaacaagcaaaaaatatgaatgatattggttgttattttttagatattcaattatcaacagatattaatggtattaaattaggttcagaaattcgtaaacatgatccagttggtaatattatttttgttacatcacattcagaattaacatatttaacatttgtttataaagttgcagcaatggattttatttttaaagatgatccagcagaattacgtacacgtattattgattgtttagaaacagcacatacacgtttacaattattatcaaaagataattcagttgaaacaattgaattaaaacgtggttcaaattcagtttatgttcaatatgatgatattatgttttttgaatcatcaacaaaatcacatcgtttaattgcacatttagataatcgtcaaattgaattttatggtaatttaaaagaattatcacaattagatgatcgtttttttcgttgtcataattcatttgttgttaatcgtcataatattgaatcaattgattcaaaagaacgtattgtttattttaaaaataaagaacattgttatgcatcagttcgtaatgttaaaaaaatttaataa BBa_K748000_sequence 1 atggaaattgcattagcaacagataatccatatgaagttttagaacaagcaaaaaatatgaatgatattggttgttattttttagatattcaattatcaacagatattaatggtattaaattaggttcagaaattcgtaaacatgatccagttggtaatattatttttgttacatcacattcagaattaacatatttaacatttgtttataaagttgcagcaatggattttatttttaaagatgatccagcagaattacgtacacgtattattgattgtttagaaacagcacatacacgtttacaattattatcaaaagataattcagttgaaacaattgaattaaaacgtggttcaaattcagtttatgttcaatatgatgatattatgttttttgaatcatcaacaaaatcacatcgtttaattgcacatttagataatcgtcaaattgaattttatggtaatttaaaagaattatcacaattagatgatcgtttttttcgttgtcataattcatttgttgttaatcgtcataatattgaatcaattgattcaaaagaacgtattgtttattttaaaaataaagaacattgttatgcatcagttcgtaatgttaaaaaaatttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z