BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_S05049 1 BBa_S05049 B0034:K748001 2012-09-14T11:00:00Z 2015-05-08T01:14:48Z false false _9_ 0 8564 9 It's complicated false false Lei Qiao component2183416 1 BBa_K748001 component2183414 1 BBa_B0034 annotation2183414 1 BBa_B0034 range2183414 1 1 12 annotation2183416 1 BBa_K748001 range2183416 1 19 1314 BBa_K748001 1 BBa_K748001 AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system. 2012-09-14T11:00:00Z 2015-05-08T01:13:12Z Staphylococcus aureus genomic sequence AgrC is a transmembrane protein, which is the sensor molecule of a typical two-component signal system in S.aureus. AgrC possesses several key amino acid motifs typical of histidine protein kinase sensor. The AgrC sensor kinase can specifically binds to corresponding AIP, which secreted only from specific S.aureus, and the composite of AgrC and AIP then leads to phosphorylation of AgrA. AgrA in its phosphorylated sate activates transcription from both P2 and P3, leading to the production of GFP and 3OC6HSL. Thus we can detect the presence of S.aureus expediently by observing the expression of GFP. false false _999_ 0 8564 9 It's complicated false we employed site-specific mutagenesis(AgrC-I A101T) to replace a amino acid residue in AgrC-I so that the mutated AgrC can bound to both AIP-I and AIP-IV. false Lei Qiao annotation2183341 1 AgrC-I A101T range2183341 1 301 303 BBa_K748001_sequence 1 atggaattattaaatagttataattttgttttattcgtattaactcaaatgatattaatgtttacaataccagctataattagtggtattaagtacagtaaacttgattattttttcatcatagtaatttcgacattatcgttatttctatttaaaatgtttgatagcgcgtccttaatcatattaacttcatttattattataatgtattttgtcaaaatcaaatggtattctattttgttgattatgacttcgcagattattctatactgtgctaattacatgtatatagttatatatacctatatcaccaaaatttctgatagtatatttgtaatattccctagcttttttgtagtttatgtgactattagtatactattctcatatataataaatagagttctcaaaaaaattagcacaccatatctaatactaaacaaaggatttttaatagttatttcgactatcttactgcttactttttcattatttttcttttattcacaaataaactcggatgaagctaaagtaataaggcagtattcttttatttttattggtatcactatatttttaagtatattaacatttgttatttctcaatttctccttaaagagatgaaatataaacgtaatcaagaagaaattgaaacctattatgaatatacattgaagattgaagctatcaacaacgaaatgcgcaagttccgtcatgattatgtcaatatcttaacgacactttcagaatacattcgagaagatgacatgcctggcctacgtgattatttcaataaaaatattgtacctatgaaagacaatttacaaatgaatgctataaaattaaatggtatcgagaatcttaaagtacgtgaaattaaaggcttaattactgcgaaaattttacgtgcacaagaaatgaatattccgattagtatcgaaatacccgatgaagtaagtagcattaacttgaatatgatcgatttaagtcgcagtattggtattattcttgataatgcaattgaggcatcaactgaaattgatgaccctatcattcgcgttgcatttattgaaagtgaaaattcagtaacgtttattgttatgaataaatgcgctgatgatataccacgcattcatgaattgttccaagaaagtttttctactaaaggtgaaggtcgtggtttaggtctatcaactttaaaagaaattgctgataatgcagacaatgtcttattagatacaattatcgaaaatggtttctttattcaaaaagttgaaattattaacaactaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_S05049_sequence 1 aaagaggagaaatactagatggaattattaaatagttataattttgttttattcgtattaactcaaatgatattaatgtttacaataccagctataattagtggtattaagtacagtaaacttgattattttttcatcatagtaatttcgacattatcgttatttctatttaaaatgtttgatagcgcgtccttaatcatattaacttcatttattattataatgtattttgtcaaaatcaaatggtattctattttgttgattatgacttcgcagattattctatactgtgctaattacatgtatatagttatatatacctatatcaccaaaatttctgatagtatatttgtaatattccctagcttttttgtagtttatgtgactattagtatactattctcatatataataaatagagttctcaaaaaaattagcacaccatatctaatactaaacaaaggatttttaatagttatttcgactatcttactgcttactttttcattatttttcttttattcacaaataaactcggatgaagctaaagtaataaggcagtattcttttatttttattggtatcactatatttttaagtatattaacatttgttatttctcaatttctccttaaagagatgaaatataaacgtaatcaagaagaaattgaaacctattatgaatatacattgaagattgaagctatcaacaacgaaatgcgcaagttccgtcatgattatgtcaatatcttaacgacactttcagaatacattcgagaagatgacatgcctggcctacgtgattatttcaataaaaatattgtacctatgaaagacaatttacaaatgaatgctataaaattaaatggtatcgagaatcttaaagtacgtgaaattaaaggcttaattactgcgaaaattttacgtgcacaagaaatgaatattccgattagtatcgaaatacccgatgaagtaagtagcattaacttgaatatgatcgatttaagtcgcagtattggtattattcttgataatgcaattgaggcatcaactgaaattgatgaccctatcattcgcgttgcatttattgaaagtgaaaattcagtaacgtttattgttatgaataaatgcgctgatgatataccacgcattcatgaattgttccaagaaagtttttctactaaaggtgaaggtcgtggtttaggtctatcaactttaaaagaaattgctgataatgcagacaatgtcttattagatacaattatcgaaaatggtttctttattcaaaaagttgaaattattaacaactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z