LOCUS       BBa_S05052               851 bp    DNA     linear       08-Jun-2024
DEFINITION  R0062:K784006
ACCESSION   BBa_S05052
VERSION     BBa_S05052.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    64..80
                     /label=Additional 5' UTR
     misc_feature    81..118
                     /label=Theophylline aptamer
     RBS             119..137
                     /label=RBS
     misc_feature    64..137
                     /label=BBa_K784005
     CDS             138..848
                     /label=mCherry
     misc_feature    489..489
                     /label=C->T (removing PstI site)
     misc_feature    138..851
                     /label=BBa_J06504
     misc_feature    64..851
                     /label=BBa_K784006
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gaggactcac tataggtacc ggtgatacca gcatcgtctt gatgcccttg gcagcaccct
      121 gctaaggtaa caacaagatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
      181 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      301 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      361 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      481 actcctcctt gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      541 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
      601 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
      661 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      721 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
      781 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      841 acaagtaata a
//
LOCUS       BBa_K784013              851 bp    DNA     linear       08-Jun-2024
DEFINITION  pLux+Theophylline riboswitch+mCherry
ACCESSION   BBa_K784013
VERSION     BBa_K784013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    64..80
                     /label=Additional 5' UTR
     misc_feature    81..118
                     /label=Theophylline aptamer
     RBS             119..137
                     /label=RBS
     misc_feature    64..137
                     /label=BBa_K784005
     CDS             138..848
                     /label=mCherry
     misc_feature    489..489
                     /label=C->T (removing PstI site)
     misc_feature    138..851
                     /label=BBa_J06504
     misc_feature    64..851
                     /label=BBa_K784006
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gaggactcac tataggtacc ggtgatacca gcatcgtctt gatgcccttg gcagcaccct
      121 gctaaggtaa caacaagatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
      181 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      301 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      361 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      481 actcctcctt gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      541 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
      601 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
      661 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      721 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
      781 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      841 acaagtaata a
//