BBa_K784013
1
BBa_K784013
pLux+Theophylline riboswitch+mCherry
2012-09-15T11:00:00Z
2015-05-08T01:13:21Z
Cloning of [[Part:BBa_K784006|theophylline riboswitch]] downstream to the [[Part:BBa_R0062|pLux promoter]]
Released HQ 2013
This sequence is the product of restriction cloning of the [[Part:BBa_K784006|theophylline riboswitch]] downstream to the [[Part:BBa_R0062|pLux promoter]]. In the presence of [[Part:BBa_I0462|LuxR]] transcription can be induced by [[3OC6HSL|3OC<sub>6</sub>HSL]].
false
true
_1039_
0
11677
9
In stock
false
No design considerations.
false
Ilya Vainberg Slutskin
component2183682
1
BBa_R0062
component2183694
1
BBa_K784006
annotation2183694
1
BBa_K784006
range2183694
1
64
851
annotation2183682
1
BBa_R0062
range2183682
1
1
55
BBa_J06504
1
mCherry
monomeric RFP optimized for bacteria
2005-07-17T11:00:00Z
2016-01-25T01:05:28Z
mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
Released HQ 2013
mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
false
false
_20_
4206
340
20
In stock
false
<p>
Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR.
</p>
<p>
Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
</p>
true
ytwang
annotation1585829
1
mCherry
range1585829
1
1
711
annotation1585833
1
C->T (removing PstI site)
range1585833
1
352
352
BBa_S05052
1
BBa_S05052
R0062:K784006
2012-09-15T11:00:00Z
2015-05-08T01:14:49Z
Released HQ 2013
true
false
_9_
0
11677
9
Discontinued
false
false
Ilya Vainberg Slutskin
component2183664
1
BBa_K784006
component2183652
1
BBa_R0062
annotation2183652
1
BBa_R0062
range2183652
1
1
55
annotation2183664
1
BBa_K784006
range2183664
1
64
851
BBa_K784006
1
BBa_K784006
Theophylline riboswitch 12.1 + mCherry
2012-09-12T11:00:00Z
2015-05-08T01:13:21Z
This part is a composite part of [[Part:BBa_K784005|BBa_K784005]] and [[Part:BBa_J06504|BBa_J06504]]
Released HQ 2013
This part is a direct fusion of the theophylline riboswitch ([[Part:BBa_K784005|BBa_K784005]]) and the mCherry protein ([[Part:BBa_J06504|BBa_J06504]]) which was amplified from [[Part:BBa_J06702|BBa_J06702]]. The part was generated by [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr]. This part was to be used in order to characterize the effect of theophylline concentration on the level of translation of the mCherry protein.
false
false
_1039_
0
11677
9
In stock
false
The theophylline riboswitch had to be directly fused to the start codon of the mCherry protein. To achieve this, [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr] was used.
false
Ilya Vainberg Slutskin
component2182907
1
BBa_K784005
component2182910
1
BBa_J06504
annotation2182907
1
BBa_K784005
range2182907
1
1
74
annotation2182910
1
BBa_J06504
range2182910
1
75
788
BBa_K784005
1
BBa_K784005
Theophylline riboswitch 12.1
2012-09-12T11:00:00Z
2015-05-08T01:13:21Z
The part was obtained from the <a href="http://www.gallivanlab.org/">Gallivan lab</a>. The description of the part was found in the following paper:
Lynch Sean A., Gallivan Justin P. 2009. A flow cytometry-based screen for synthetic riboswitches. Nucleic Acids Research 37(1): 184-192.
A riboswitch which is comprised of an aptamer which recognizes the ligand, theophylline. The riboswitch also contains an RBS. In the absence of theophylline, there is extensive base pairing in the 5' untranslated region (UTR) which blocks the RBS. This base pairing prevents translation of a gene downstream to the riboswitch. In the presence of theophylline, the transcript adopts a secondary structure in which the RBS is exposed, allowing translation to occur.
false
false
_1039_
0
11677
9
Not in stock
false
The part must be directly fused to the starting codon of the downstream coding region.
false
Ilya Vainberg Slutskin
annotation2182924
1
RBS
range2182924
1
56
74
annotation2182922
1
Additional 5' UTR
range2182922
1
1
17
annotation2182923
1
Theophylline aptamer
range2182923
1
18
55
BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
true
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2045
1
LuxR/HSL
range2045
1
1
20
annotation2046
1
-35
range2046
1
20
25
annotation2047
1
-10
range2047
1
42
47
annotation7070
1
BBa_R0062
range7070
1
1
55
annotation2048
1
start
range2048
1
53
53
BBa_R0062_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_J06504_sequence
1
atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
BBa_K784005_sequence
1
gactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaag
BBa_K784006_sequence
1
gactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
BBa_K784013_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagaggactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
BBa_S05052_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagaggactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z