LOCUS       BBa_S05053              2418 bp    DNA     linear       05-Nov-2024
DEFINITION  S03419:B0015
ACCESSION   BBa_S05053
VERSION     BBa_S05053.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..2256
                     /label=Cph8
     misc_feature    2257..2281
                     /label=Help:Barcodes
     CDS             19..2256
                     /label=BBa_I15010
     misc_feature    1..2281
                     /label=BBa_S03419
     stem_loop       2301..2344
                     /label=stem_loop
     misc_feature    2290..2369
                     /label=BBa_B0010
     terminator      2290..2369
                     /label=BBa_B0010
     stem_loop       2385..2404
                     /label=T7 TE
     misc_feature    2411..2411
                     /label=stop
     polyA_site      2405..2418
                     /label=polya
     misc_feature    2378..2418
                     /label=BBa_B0012
     terminator      2378..2418
                     /label=BBa_B0012
     terminator      2290..2418
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat ggccaccacc gtacaactca gcgaccaatc cctccgtcag
       61 ctagaaaccc tcgccatcca caccgcccac ctgattcagc cccacggttt agtggtggtc
      121 ctgcaagaac cagacctcac catcagccaa attagcgcca actgcaccgg cattttaggg
      181 cgatcgccag aggatttgtt gggcagaacc ctaggggaag tgtttgatag ctttcagatt
      241 gatcccatcc agagtcgcct aacggccgga caaatcagca gcctcaaccc cagtaaactt
      301 tgggcgcggg tcatggggga cgactttgtc atttttgacg gggtttttca tcgcaacagt
      361 gacggtttat tggtatgtga actcgagcca gcctacactt ccgataatct gcccttcctc
      421 ggtttttatc acatggccaa cgctgccctg aatcggttgc gccaacaagc taatctacgg
      481 gatttctacg atgttattgt cgaagaagtc cgccgtatga ctggctttga ccgggtgatg
      541 ctataccgct ttgatgaaaa taaccacggt gatgtcattg ccgaagataa acgggatgat
      601 atggaaccct atttgggcct gcactatccc gaatcggata ttccccaacc cgcccgtcgg
      661 ctatttatcc acaaccccat tcgagtaatt cccgatgttt atggtgtggc ggtgcccctg
      721 accccagcgg ttaaccccag caccaaccga gcggtggatt taacagaatc cattctgcgc
      781 agtgcgtacc attgccactt gacctatctg aaaaatatgg gggtaggagc gtctttaacc
      841 atttccctaa ttaaggacgg ccatctctgg gggctcattg cctgccacca tcaaaccccc
      901 aaagtaattc cctttgaact gcgtaaagcc tgcgaatttt ttggtcgggt ggtgtttagc
      961 aacatttccg cccaggaaga tacggaaacc ttcgattacc gggtgcagct ggcggagcat
     1021 gaagcggttt tattggacaa aatgaccacg gcggcggatt ttgtcgaagg attaactaat
     1081 catcccgatc gcctgttggg attaacgggc tcccaggggg cggccatttg ctttggggaa
     1141 aaattgattt tagtagggga aaccccggac gagaaagcag tgcaatattt actgcaatgg
     1201 ttggagaatc gggaagtgca agacgttttc ttcacctctt ccctctcaca aatttatcct
     1261 gatgcagtga attttaaatc cgtggccagt ggcttattgg ccattcccat tgcccgtcac
     1321 aactttttgc tctggtttcg ccctgaagtg ttgcaaacgg ttaattgggg cggtgaccca
     1381 aatcatgctt acgaagctac ccaggaagac ggtaaaatcg agctccatcc ccgccaatcc
     1441 tttgacctct ggaaagaaat tgtccgactc caatctttgc cctggcaatc ggtggaaatc
     1501 caaagtgccc tggccctgaa aaaggcgatc gtcaacctca ttttgcgcca ggcagaagaa
     1561 ttgcatatgg cggctggtgt taagcaactg gcggatgacc gcacgctgct gatggcgggg
     1621 gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc tggcgactga gatgatgagc
     1681 gagcaggatg gctatctggc agaatcgatc aataaagata tcgaagagtg caacgccatc
     1741 attgagcagt ttatcgacta cctgcgcacc gggcaggaga tgccgatgga aatggcggat
     1801 cttaatgcag tactcggtga ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa
     1861 accgcgcttt accccggcag cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg
     1921 gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg gctgggtcaa agtcagcagc
     1981 ggaacggagc cgaatcgcgc ctggttccag gtggaagatg acggtccggg aattgcgccg
     2041 gaacaacgta agcacctgtt ccagccgttt gtccgcggcg acagtgcgcg caccattagc
     2101 ggcacgggat tagggctggc aattgtgcag cgtatcgtgg ataaccataa cgggatgctg
     2161 gagcttggca ccagcgagcg gggcgggctt tccattcgcg cctggctgcc agtgccggta
     2221 acgcgggcgc agggcatgac aaaagaaggg taataactct gatagtgcta gtgtagatct
     2281 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2341 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2401 ggcctttctg cgtttata
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