LOCUS       BBa_S05054              1828 bp    DNA     linear       23-May-2024
DEFINITION  K081024:I13507
ACCESSION   BBa_S05054
VERSION     BBa_S05054.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..771
                     /label=PcyA
     misc_feature    772..796
                     /label=Help:Barcodes
     CDS             22..796
                     /label=BBa_I15009
     misc_feature    805..843
                     /label=B1006
     stem_loop       814..835
                     /label=modified thr terminator
     polyA_site      836..843
                     /label=PolyA
     polyA_site      805..813
                     /label=PolyA
     terminator      805..843
                     /label=BBa_B1006
     misc_feature    864..881
                     /label=C1 OmpR
     misc_feature    885..902
                     /label=C2 OmpR
     misc_feature    905..922
                     /label=C3 OmpR
     promoter        926..931
                     /label=-35
     promoter        949..954
                     /label=-10
     promoter        852..959
                     /label=BBa_R0082
     misc_feature    1..959
                     /label=BBa_K081024
     misc_feature    972..975
                     /label=conserved
     RBS             968..979
                     /label=BBa_B0034
     CDS             986..1660
                     /label=mrfp1
     misc_feature    1667..1691
                     /label=Help:Barcodes
     CDS             986..1666
                     /label=BBa_E1010
     stem_loop       1711..1754
                     /label=stem_loop
     misc_feature    1700..1779
                     /label=BBa_B0010
     terminator      1700..1779
                     /label=BBa_B0010
     stem_loop       1795..1814
                     /label=T7 TE
     misc_feature    1821..1821
                     /label=stop
     polyA_site      1815..1828
                     /label=polya
     misc_feature    1788..1828
                     /label=BBa_B0012
     terminator      1788..1828
                     /label=BBa_B0012
     misc_feature    968..1828
                     /label=BBa_I13507
ORIGIN
        1 attaaagagg agaaatacta gatggccgtc actgatttaa gtttgaccaa ttcttccctg
       61 atgcctacgt tgaacccgat gattcaacag ttggccctgg cgatcgccgc tagttggcaa
      121 agtttacccc tcaagcccta tcaattgccg gaggatttgg gctacgtaga aggccgcctg
      181 gaaggggaaa agttagtgat tgaaaatcgg tgctaccaaa cgccccagtt tcgcaaaatg
      241 catttggagt tggccaaggt gggcaaaggg ttggatattc tccactgtgt aatgtttcct
      301 gagcctttat acggtctacc tttgtttggc tgtgacattg tggccggccc cggtggagta
      361 agtgcggcta ttgcggatct atcccccacc caaagcgatc gccaattgcc cgcagcgtac
      421 caaaaatcat tggcagagct aggccagcca gaatttgagc aacaacggga attgcccccc
      481 tggggagaaa tattttctga atattgttta ttcatccgtc ccagcaatgt cactgaagaa
      541 gaaagatttg tacaaagggt agtggacttt ttgcaaattc attgtcacca atccatcgtt
      601 gccgaaccct tgtctgaagc tcaaactttg gagcaccgtc aggggcaaat tcattactgc
      661 caacaacaac agaaaaatga taaaacccgt cgggtactgg aaaaagcttt tggggaagct
      721 tgggcggaac ggtatatgag ccaagtctta tttgatgtta tccaataata actctgatag
      781 tgctagtgta gatctctact agagaaaaaa aaaccccgcc cctgacaggg cggggttttt
      841 ttttactaga gtcccttgca tttacatttt gaaacatcta tagcgataaa tgaaacatct
      901 taaaagtttt agtatcatat tcgtgttgga ttattctgca tttttgggga gaatggactt
      961 actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat
     1021 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
     1081 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
     1141 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
     1201 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
     1261 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
     1321 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
     1381 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
     1441 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
     1501 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
     1561 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
     1621 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
     1681 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1741 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1801 ccttcgggtg ggcctttctg cgtttata
//