##gff-version 3
##sequence-region BBa_S05054 1 1828
BBa_S05054	.	engineered_region	1	959	.	+	0	ID=BBa_K081024;Name=BBa_K081024
BBa_S05054	.	promoter	852	959	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_K081024
BBa_S05054	.	non_covalent_binding_site	905	922	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_S05054	.	non_covalent_binding_site	864	881	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_S05054	.	promoter	949	954	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_S05054	.	non_covalent_binding_site	885	902	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_S05054	.	promoter	926	931	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_S05054	.	CDS	22	796	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_K081024
BBa_S05054	.	CDS	22	771	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_S05054	.	engineered_tag	772	796	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_S05054	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081024
BBa_S05054	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S05054	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S05054	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S05054	.	terminator	805	843	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081024
BBa_S05054	.	polyA_site	805	813	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_S05054	.	engineered_region	805	843	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_S05054	.	polyA_site	836	843	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_S05054	.	stem_loop	814	835	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_S05054	.	engineered_region	968	1828	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_S05054	.	CDS	986	1666	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_S05054	.	engineered_tag	1667	1691	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S05054	.	CDS	986	1660	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S05054	.	ribosome_entry_site	968	979	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_S05054	.	conserved_region	972	975	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05054	.	terminator	1700	1779	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_S05054	.	stem_loop	1711	1754	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05054	.	engineered_region	1700	1779	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05054	.	terminator	1788	1828	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_S05054	.	polyA_site	1815	1828	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05054	.	stop_codon	1821	1821	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05054	.	stem_loop	1795	1814	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05054	.	engineered_region	1788	1828	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_S05054
attaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccga
tgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggattt
gggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaa
atgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtc
tacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaag
cgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattg
cccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtac
aaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttgga
gcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagctttt
ggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgta
gatctctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagtcccttgcatttacattt
tgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggg
gagaatggacttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttc
aaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggta
cccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagta
cggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgg
gaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatct
acaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttc
caccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcac
tacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaac
tggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgc
ttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata