LOCUS BBa_K873013 1699 bp DNA linear 25-Nov-2024 DEFINITION cujugative transfer death gene ACCESSION BBa_K873013 VERSION BBa_K873013.1 FEATURES Location/Qualifiers oriT 1..371 /label=BBa_J01003 misc_feature 380..398 /label=TetR 1 misc_feature 380..433 /label=BBa_R0040 promoter 399..404 /label=-35 misc_feature 405..423 /label=TetR 2 promoter 422..427 /label=-10 promoter 380..433 /label=BBa_R0040 misc_feature 446..449 /label=conserved RBS 442..453 /label=BBa_B0034 CDS 460..1134 /label=mrfp1 misc_feature 1141..1165 /label=Help:Barcodes CDS 460..1140 /label=BBa_E1010 stem_loop 1185..1228 /label=stem_loop misc_feature 1174..1253 /label=BBa_B0010 terminator 1174..1253 /label=BBa_B0010 stem_loop 1269..1288 /label=T7 TE misc_feature 1295..1295 /label=stop polyA_site 1289..1302 /label=polya misc_feature 1262..1302 /label=BBa_B0012 terminator 1262..1302 /label=BBa_B0012 misc_feature 380..1302 /label=BBa_I13521 oriT 1..1302 /label=BBa_K873003 RBS 1422..1433 /label=RBS BBa_B0034 promoter 1311..1412 /label=BBa_K117002 CDS 1419..1562 /label=BBa_K117000 stem_loop 1582..1625 /label=stem_loop misc_feature 1571..1650 /label=BBa_B0010 terminator 1571..1650 /label=BBa_B0010 stem_loop 1666..1685 /label=T7 TE misc_feature 1692..1692 /label=stop polyA_site 1686..1699 /label=polya misc_feature 1659..1699 /label=BBa_B0012 terminator 1659..1699 /label=BBa_B0012 misc_feature 1311..1699 /label=BBa_K117010 ORIGIN 1 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg 61 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg 121 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa 181 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc 241 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat 301 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat 361 gcagcggaaa atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag 421 agatactgag cactactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt 481 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc 541 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa 601 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac 661 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc 721 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt 781 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc 841 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc 901 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg 961 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt 1021 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac 1081 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa 1141 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc 1201 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 1261 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag cacaacatca 1321 actgagtgca tgccttattt aatcatgtaa ctattcttac gacttcaaac gtcaaacagt 1381 cttaacactt atttaattaa aaagaggaga aatactagat gaaaaaaata acagggatta 1441 ttttattgct tcttgcagcc attattcttg ctgcatgtca ggcaaactat atccgtgatg 1501 ttcagggcgg gacagtatca ccgtcgtcaa ctgctgaact gaccggagtg gaaacgcagt 1561 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 1621 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 1681 gggcctttct gcgtttata // LOCUS BBa_S05059 1699 bp DNA linear 25-Nov-2024 DEFINITION K873003:K117010 ACCESSION BBa_S05059 VERSION BBa_S05059.1 FEATURES Location/Qualifiers oriT 1..371 /label=BBa_J01003 misc_feature 380..398 /label=TetR 1 misc_feature 380..433 /label=BBa_R0040 promoter 399..404 /label=-35 misc_feature 405..423 /label=TetR 2 promoter 422..427 /label=-10 promoter 380..433 /label=BBa_R0040 misc_feature 446..449 /label=conserved RBS 442..453 /label=BBa_B0034 CDS 460..1134 /label=mrfp1 misc_feature 1141..1165 /label=Help:Barcodes CDS 460..1140 /label=BBa_E1010 stem_loop 1185..1228 /label=stem_loop misc_feature 1174..1253 /label=BBa_B0010 terminator 1174..1253 /label=BBa_B0010 stem_loop 1269..1288 /label=T7 TE misc_feature 1295..1295 /label=stop polyA_site 1289..1302 /label=polya misc_feature 1262..1302 /label=BBa_B0012 terminator 1262..1302 /label=BBa_B0012 misc_feature 380..1302 /label=BBa_I13521 oriT 1..1302 /label=BBa_K873003 RBS 1422..1433 /label=RBS BBa_B0034 promoter 1311..1412 /label=BBa_K117002 CDS 1419..1562 /label=BBa_K117000 stem_loop 1582..1625 /label=stem_loop misc_feature 1571..1650 /label=BBa_B0010 terminator 1571..1650 /label=BBa_B0010 stem_loop 1666..1685 /label=T7 TE misc_feature 1692..1692 /label=stop polyA_site 1686..1699 /label=polya misc_feature 1659..1699 /label=BBa_B0012 terminator 1659..1699 /label=BBa_B0012 misc_feature 1311..1699 /label=BBa_K117010 ORIGIN 1 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg 61 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg 121 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa 181 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc 241 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat 301 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat 361 gcagcggaaa atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag 421 agatactgag cactactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt 481 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc 541 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa 601 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac 661 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc 721 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt 781 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc 841 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc 901 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg 961 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt 1021 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac 1081 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa 1141 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc 1201 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 1261 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag cacaacatca 1321 actgagtgca tgccttattt aatcatgtaa ctattcttac gacttcaaac gtcaaacagt 1381 cttaacactt atttaattaa aaagaggaga aatactagat gaaaaaaata acagggatta 1441 ttttattgct tcttgcagcc attattcttg ctgcatgtca ggcaaactat atccgtgatg 1501 ttcagggcgg gacagtatca ccgtcgtcaa ctgctgaact gaccggagtg gaaacgcagt 1561 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 1621 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 1681 gggcctttct gcgtttata //