LOCUS       BBa_S05073              1104 bp    DNA     linear       29-Sep-2024
DEFINITION  B0015:P1007
ACCESSION   BBa_S05073
VERSION     BBa_S05073.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     CDS             138..956
                     /label=kanamycin resistance
     misc_feature    924..924
                     /label=G to C mutation to eliminate XhoI site
     misc_feature    1061..1104
                     /label=repeat region
     misc_feature    138..1104
                     /label=BBa_P1007
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtta ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      181 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      241 aactcaccga ggcagttcca aagaatggca aggtcctggt aacggtctgc gattccgacc
      301 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      361 aaatcaccat gagtgacgac tgaatccggt gagaatggca agagcttgtg catttctttc
      421 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
      481 ccgttattca tgcgtgattg cgcctgagca agacgaaata cacgatcgct gttaaaagga
      541 caattacaaa caggaatcga atgtaaccgg cgcaggaaca cggccagcgc atcaacaata
      601 ttttcacctg aatcaggata ttcttctaat acctggaagg ctgttttccc aggaatcgcg
      661 gtggtgagta accacgcatc atcaggagta cggataaaat gcttgatggt cgggagaggc
      721 ataaactccg tcagccagtt gagacggacc atctcatctg taacatcatt ggcaacgcta
      781 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt
      841 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcgtcc
      901 atgttggagt ttaagcgcgg acgggagcaa gacgtttccc gttgaatatg gctcataaca
      961 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     1021 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     1081 acttttgctg agttgaagga tcag
//