BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_K874001 1 BBa_K874001 Polydactyl Zinc Finger (PZF3838) 2012-09-18T11:00:00Z 2015-05-08T01:13:38Z Synthesized by IDT. For more information see individual components descriptions. Intermediate construct used in the iGEM Amsterdam 2012 project. false true _1135_ 0 12435 9 Not in stock false See Individual components false Ernst Bank annotation2192156 1 start range2192156 1 4 6 annotation2200695 1 Zinc Finger 1 range2200695 1 7 90 annotation2192157 1 Zinc Finger Array range2192157 1 7 510 annotation2200707 1 Zinc Finger 6 range2200707 1 427 510 annotation2200705 1 Zinc Finger 5 range2200705 1 343 426 annotation2200700 1 Zinc Finger 3 range2200700 1 175 258 annotation2200698 1 Zinc Finger 2 range2200698 1 91 174 annotation2200703 1 Zinc Finger 4 range2200703 1 259 342 BBa_K874000 1 BBa_K874000 M.ScaI Methyltransferase 2012-09-16T11:00:00Z 2015-05-08T01:13:38Z Natively found in Streptomyces caespitosus. Sequence obtained from REBASE. Synthesized by IDT. The M.ScaI protein is a type II methyltransferase (subtype beta) that recognises site on the DNA of the following sequence 5..AGTACT..3. It methylates this site at the 5th (Cytosine) nucleotide leaving ao an N4-methylcytosine (m4). This methylation type (m4) is not found in native E. coli nor is the recognition site methylated by any of E. coli's native methylation systems (Dam, Dcm). This part also includes a flexible linker with incorporated myc-tag at the N terminus of the protein. This will allow for easier creation of fusion proteins and expression verification using westerblot (using the myc antibodies). It can be assumed that M.ScaI (with linker) is expressed and folds properly in E. coli because its function has been verified by the iGEM Amsterdam 2012 team. false false _1135_ 0 12435 9 Not in stock true Contains no forbidden sites (according to the RFC-10 protocol). Contains no sites vulnerable to E. coli's native restriction systems. false Ernst Bank annotation2187005 1 stop range2187005 1 913 915 annotation2187003 1 start range2187003 1 1 3 annotation2187004 1 M.ScaI range2187004 1 1 912 BBa_S05074 1 BBa_S05074 B0032:K874021 2012-09-21T11:00:00Z 2015-05-08T01:14:49Z false false _9_ 0 12435 9 Not in stock false false Ernst Bank component2192169 1 BBa_K874001 component2192172 1 BBa_K874021 component2192165 1 BBa_B0032 component2192176 1 BBa_K874000 annotation2192169 1 BBa_K874001 range2192169 1 14 523 annotation2192176 1 BBa_K874000 range2192176 1 578 1492 annotation2192165 1 BBa_B0032 range2192165 1 1 13 annotation2192172 1 BBa_K874021 range2192172 1 524 577 BBa_K874021 1 BBa_K874021 Myc-Linker 2012-09-21T11:00:00Z 2015-05-08T01:13:38Z None yet None yet false false _1135_ 0 12435 9 Not in stock false None yet false Ernst Bank annotation2191557 1 Linker range2191557 1 1 54 annotation2191558 1 Myc range2191558 1 16 45 BBa_K874001_sequence 1 cgcatgggcgaaaagccgtatgcgtgccccgaatgtgggaagtccttttctcgtagcgacgagcttgttcgccaccaaaggacccataccggcgagaagccctacgcctgcccggagtgtggaaagtcgttctcccagagcggcgatttgcgtcggcatcaacggacgcataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgagaaaccatacgcctgtcccgagtgcggtaaaagctttagtcagcgtgcccatcttgaacgccaccagaggacccataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgaaaaaccgtatgcttgtccagaatgtggtaagtccttttctcgtagcgataagctcgttcgccaccaaaggactcatacc BBa_K874021_sequence 1 gccgccagctttcaagaacagaaactcatctctgaagaggatctggcacccggc BBa_K874000_sequence 1 atgtccgggcgggactttggatatgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggcttgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgagctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagggtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcggcgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggacaagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgcgcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgtgaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttccccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattgaacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgaccgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggaggcggaggcgcaatccctcttctag BBa_S05074_sequence 1 tcacacaggaaagcgcatgggcgaaaagccgtatgcgtgccccgaatgtgggaagtccttttctcgtagcgacgagcttgttcgccaccaaaggacccataccggcgagaagccctacgcctgcccggagtgtggaaagtcgttctcccagagcggcgatttgcgtcggcatcaacggacgcataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgagaaaccatacgcctgtcccgagtgcggtaaaagctttagtcagcgtgcccatcttgaacgccaccagaggacccataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgaaaaaccgtatgcttgtccagaatgtggtaagtccttttctcgtagcgataagctcgttcgccaccaaaggactcataccgccgccagctttcaagaacagaaactcatctctgaagaggatctggcacccggcatgtccgggcgggactttggatatgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggcttgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgagctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagggtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcggcgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggacaagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgcgcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgtgaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttccccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattgaacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgaccgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggaggcggaggcgcaatccctcttctag BBa_B0032_sequence 1 tcacacaggaaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z