LOCUS       BBa_S05084              1999 bp    DNA     linear       07-Dec-2025
DEFINITION  F2620:S05083
ACCESSION   BBa_S05084
VERSION     BBa_S05084.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1091..1798
                     /label=cI lambda
     misc_feature    1799..1831
                     /label=LVA
     misc_feature    1838..1862
                     /label=Help:Barcodes
     CDS             1088..1862
                     /label=BBa_C0051
     misc_feature    1070..1862
                     /label=BBa_S05082
     stem_loop       1882..1925
                     /label=stem_loop
     misc_feature    1871..1950
                     /label=BBa_B0010
     terminator      1871..1950
                     /label=BBa_B0010
     stem_loop       1966..1985
                     /label=T7 TE
     misc_feature    1992..1992
                     /label=stop
     polyA_site      1986..1999
                     /label=polya
     misc_feature    1959..1999
                     /label=BBa_B0012
     terminator      1959..1999
                     /label=BBa_B0012
     terminator      1871..1999
                     /label=BBa_B0015
     misc_feature    1070..1999
                     /label=BBa_S05083
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg
     1141 ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc
     1201 agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt
     1261 aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg aagaatttag
     1321 cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact
     1381 tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctga
     1441 gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag
     1501 tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa
     1561 gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg
     1621 tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga
     1681 tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga
     1741 gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc
     1801 tgcaaacgac gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc
     1861 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1921 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1981 gggcctttct gcgtttata
//