LOCUS BBa_S05085 3751 bp DNA linear 15-Dec-2024 DEFINITION K598002:F1610 ACCESSION BBa_S05085 VERSION BBa_S05085.1 FEATURES Location/Qualifiers CDS 1..681 /label=mRFP RBS 682..693 /label=RBS CDS 708..1421 /label=lamda CI repressor RBS 1422..1433 /label=RBS promoter 1440..1491 /label=PRM (CI434 regulated promoter) promoter 1492..1540 /label=PR (CI regulated promoter) RBS 1556..1567 /label=RBS CDS 1568..2203 /label=CI434 repressor misc_feature 2214..2225 /label=RBS CDS 2226..2945 /label=eGFP misc_feature 1..2945 /label=BBa_K598002 misc_feature 2958..2961 /label=conserved RBS 2954..2965 /label=BBa_B0034 misc_feature 3551..3582 /label=LVA CDS 2972..3550 /label=luxI misc_feature 2972..3589 /label=BBa_C0061 misc_feature 3590..3614 /label=Help:Barcodes CDS 2972..3589 /label=BBa_C0061 stem_loop 3634..3677 /label=stem_loop misc_feature 3623..3702 /label=BBa_B0010 terminator 3623..3702 /label=BBa_B0010 stem_loop 3718..3737 /label=T7 TE misc_feature 3744..3744 /label=stop polyA_site 3738..3751 /label=polya misc_feature 3711..3751 /label=BBa_B0012 terminator 3711..3751 /label=BBa_B0012 misc_feature 2954..3751 /label=BBa_F1610 ORIGIN 1 ttattaagca ccggtggagt gacgaccttc agcacgttcg tactgttcaa cgatggtgta 61 gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg 121 aaccggtttt ttagccatgt aggtggtttt aacttcagcg tcgtagtgac caccgtcttt 181 cagtttcaga cgcattttga tttcaccttt cagagcaccg tcttccgggt acatacgttc 241 ggtggaagct tcccaaccca tggttttttt ctgcataacc ggaccgtcgg acgggaagtt 301 ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt 361 aacggtaaca acaccaccgt cttcgaagtt cataacacgt tcccatttga aaccttccgg 421 gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc 481 gtactggaac tgcggggaca ggatgtccca agcgaacggc agcggaccac ctttggtaac 541 tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac cttcgatttc 601 gaactcgtga ccgctaacgg aaccttccat acgaactttg aaacgcatga actctttgat 661 aacgtcttcg gaggaagcca tctagtattt ctcctctttc actagaatca gccaaacgtc 721 tcttcaggcc actgactagc gataactttc cccacaacgg aacaactctc attgcatggg 781 atcattgggt actgtgggtt tagtggttgt aaaaacgcct gaccgctatc cctgatcagt 841 ttcttgaagg taaactcatc acccccaagt ctggctatgc agaaatcacc tggctcaaca 901 gcctgctcag ggtcaacgag aattaacatt ccgtcaggaa agcttggctt ggagcctgtt 961 ggtgcggtca tggaattacc ttcaacctca agccagaatg cagaatcact ggcttttttg 1021 gttgtgctta cccatctctc cgcatcacct ttggtaaagg ttctaagctc aggtgagaac 1081 atccctgcct gaacatgaga aaaaacaggg tactcatgct cacttctaag tgacggctgc 1141 atactaaccg ctttatacat ctcgtagatt tctctggcga ttgaagggct aaattcttca 1201 acgctaactt tgagaatttt tgcaagcaat gcggcgttat aagcatttaa tgcattgatg 1261 ccattaaata aagcaccaac gcctgactgc cccatcccca tcttgtctgc gacagattcc 1321 tgggataagc caagttcatt tttctttttt tcataaattg ctttaaggcg acgtgcgtcc 1381 tcaagctgct cttgtgttaa tggtttcttt tttgtgctca tttttttatc agtactagaa 1441 acaaactttc ttgtacatac gttaaatcta acaaactttc ttgtaaatat ctaacaccgt 1501 gcgtgttgac tattttacct ctggcggtga taatggttgc tactagagaa agtacggtac 1561 tggttgtatg agtatttctt ccagggtaaa aagcaaaaga atccagcttg gacttaacca 1621 ggctgaactt gctcaaaagg tggggactac ccagcagtct atagagcagc tcgaaaacgg 1681 taaaactaag cgaccacgct ttttaccaga acttgcgtca gctcttggcg taagtgttga 1741 ctggctgctc aatggcacct ctgattcgaa tgttagattt gttgggcacg ttgagcccaa 1801 agggaaatat ccattgatta gcatggttag agctggttcg tggtgtgaag cttgtgaacc 1861 ctacgatatc aaggacattg atgaatggta tgacagtgac gttaacttat taggcaatgg 1921 attctggctg aaagttgaag gtgattccat gacctcacct gtaggtcaaa gcatccctga 1981 aggtcatatg gtgttagtag atactggacg ggagccagtg aatggaagcc ttgttgtagc 2041 caaactgact gacgcgaacg aagcaacatt caagaaactg gtcatagatg gcggtcagaa 2101 gtacctgaaa ggcctgaacc cttcatggcc tatgactcct atcaacggaa actgcaagat 2161 tatcggtgtt gtcgtggaag cgagggtaaa attcgtataa taaactagaa gaggagaaat 2221 taagcatggt aagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 2281 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 2341 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 2401 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 2461 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 2521 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 2581 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 2641 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 2701 agagcggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtacagc 2761 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 2821 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 2881 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 2941 agtaatacta gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt 3001 tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa 3061 gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa 3121 ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg 3181 tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca 3241 acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa 3301 tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg aagctatata 3361 taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga 3421 gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt 3481 attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc 3541 agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 3601 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 3661 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 3721 tcaccttcgg gtgggccttt ctgcgtttat a //