##gff-version 3
##sequence-region BBa_S05085 1 3751
BBa_S05085	.	engineered_region	1	2945	.	+	0	ID=BBa_K598002;Name=BBa_K598002
BBa_S05085	.	ribosome_entry_site	1556	1567	.	+	0	ID=annotation2150247;Name=RBS;Parent=BBa_K598002
BBa_S05085	.	promoter	1492	1540	.	+	0	ID=annotation2150246;Name=PR (CI regulated promoter);Parent=BBa_K598002
BBa_S05085	.	sequence_feature	2214	2225	.	+	0	ID=annotation2150249;Name=RBS;Parent=BBa_K598002
BBa_S05085	.	ribosome_entry_site	682	693	.	+	0	ID=annotation2150237;Name=RBS;Parent=BBa_K598002
BBa_S05085	.	CDS	708	1421	.	+	0	ID=annotation2150238;Name=lamda CI repressor;Parent=BBa_K598002
BBa_S05085	.	promoter	1440	1491	.	+	0	ID=annotation2150243;Name=PRM (CI434 regulated promoter);Parent=BBa_K598002
BBa_S05085	.	ribosome_entry_site	1422	1433	.	+	0	ID=annotation2150239;Name=RBS;Parent=BBa_K598002
BBa_S05085	.	CDS	2226	2945	.	+	0	ID=annotation2150250;Name=eGFP;Parent=BBa_K598002
BBa_S05085	.	CDS	1	681	.	+	0	ID=annotation2150236;Name=mRFP;Parent=BBa_K598002
BBa_S05085	.	CDS	1568	2203	.	+	0	ID=annotation2150248;Name=CI434 repressor;Parent=BBa_K598002
BBa_S05085	.	engineered_region	2954	3751	.	+	0	ID=BBa_F1610;Name=BBa_F1610
BBa_S05085	.	terminator	3623	3702	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_S05085	.	stem_loop	3634	3677	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05085	.	engineered_region	3623	3702	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05085	.	terminator	3711	3751	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_S05085	.	stem_loop	3718	3737	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05085	.	stop_codon	3744	3744	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05085	.	engineered_region	3711	3751	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S05085	.	polyA_site	3738	3751	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05085	.	ribosome_entry_site	2954	2965	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_S05085	.	conserved_region	2958	2961	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05085	.	CDS	2972	3589	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_S05085	.	engineered_tag	3590	3614	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_S05085	.	engineered_region	2972	3589	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_S05085	.	CDS	2972	3550	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_S05085	.	tag	3551	3582	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
>BBa_S05085
ttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtg
atgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcag
cgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacg
ttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagttta
actttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataa
cacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagcttt
ggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcg
gtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgctaacggaaccttccatac
gaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcctctttcactagaatcag
ccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggt
actgtgggtttagtggttgtaaaaacgcctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaag
tctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggc
ttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgc
ttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaac
agggtactcatgctcacttctaagtgacggctgcatactaaccgctttatacatctcgtagatttctctggcgattgaa
gggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccat
taaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcattttt
ctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatt
tttttatcagtactagaaacaaactttcttgtacatacgttaaatctaacaaactttcttgtaaatatctaacaccgtg
cgtgttgactattttacctctggcggtgataatggttgctactagagaaagtacggtactggttgtatgagtatttctt
ccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtc
tatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgtt
gactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattga
ttagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacag
tgacgttaacttattaggcaatggattctggctgaaagttgaaggtgattccatgacctcacctgtaggtcaaagcatc
cctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacg
cgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaacccttcatggcctat
gactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataaactagaaga
ggagaaattaagcatggtaagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgac
gtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatct
gcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgcta
ccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttc
aaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagg
gcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcat
ggccgacaagcagaagagcggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtacagctcgcc
gaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccg
ccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcgg
catggacgagctgtacaagtaatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttt
tggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggactt
agttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaa
aatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtc
aacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataa
ctctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgta
acagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattc
atgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgc
aaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata