BBa_K944002 1 BBa_K944002 Transcriptional Regulator for Cyanide Inducible Promoter 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This part came from the genomic sequence of Pseudomonas pseudoalcaligenes CECT5344 and was synthesized by IDT technologies. The cynF gene codes for a sigma ?54-dependent transcriptional regulator of 648-amino-acid residues, which belongs to the FIS (factor for inversion stimulation) family of regulatory proteins. P. pseudoalcaligenes CynF shared 59% identity with R. palustris CynF (YP_485671) and 58% identity with Bradyrhizobium japonicum CynF (NP_772375). This Transcriptional regulator works along with the cyanide and cyanide compounds inducible promoter in the natural ocurring cyanase operon from P. pseudoalcaligenes. false false _1211_ 0 7118 9 It's complicated false This part had 3 illegal Pst1 restriction sites. false Natasha G??mez annotation2199373 1 Transcriptional Regulator range2199373 1 1 1932 annotation2199372 1 cynF range2199372 1 1 1932 annotation2199378 1 stop codon range2199378 1 1930 1932 annotation2199377 1 silent mutation range2199377 1 1277 1277 annotation2199376 1 silent mutation range2199376 1 1253 1253 annotation2199374 1 start codon range2199374 1 1 3 annotation2199375 1 silent mutation range2199375 1 890 890 BBa_S05090 1 BBa_S05090 K944000:K944002 2012-09-25T11:00:00Z 2015-05-08T01:14:49Z false false _9_ 0 7118 9 Not in stock false false Natasha G??mez component2202154 1 BBa_K944000 component2202162 1 BBa_K944002 annotation2202154 1 BBa_K944000 range2202154 1 1 270 annotation2202162 1 BBa_K944002 range2202162 1 277 2208 BBa_K944000 1 BBa_K944000 Putative Promoter Induced by Cyanide Compounds 2012-09-24T11:00:00Z 2015-05-08T01:13:48Z This part came from the genomic sequence Pseudomonas pseudoalcaligenes CECT5344 and was synthesized by IDT technologies. This part works with the transcriptional regulator of the Cyanide operon from Pseudomonas pseudoalcaligenes CECT5344.It is negatively regulated by ammonium and positively regulated by cyanate, cyanide, and some cyanometallic complexes. false false _1211_ 0 7118 9 It's complicated false This promoter is known to be in the cyanase operon from Pseudomonas pseudoalcaligenes CECT5344 between the cynF which is the transcriptional regulator and CynA, but its specific sequence is unknown, so our team use the sequence between this two genes to be sure that we get our inducible promoter. false Natasha G??mez BBa_S05090_sequence 1 tcgtgatcactcctgcgatggcctgccccaaattggtgaggcgggcgctgactgggctgggccattgctgaccggttcatgtcagctattgcaggagctgtaccagttcaaaaatctatataagcgattgaaaaatataaacttttaacaaaaccaataaatacgaaatctcgttattcacgagatttcgtcatcttgaaaaacgagatatcgtcaactggcacgccctttggataacgtcctgaaaggtcaatcccgagagcgccgcaatactagatgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtag BBa_K944000_sequence 1 tcgtgatcactcctgcgatggcctgccccaaattggtgaggcgggcgctgactgggctgggccattgctgaccggttcatgtcagctattgcaggagctgtaccagttcaaaaatctatataagcgattgaaaaatataaacttttaacaaaaccaataaatacgaaatctcgttattcacgagatttcgtcatcttgaaaaacgagatatcgtcaactggcacgccctttggataacgtcctgaaaggtcaatcccgagagcgccgcaa BBa_K944002_sequence 1 atgcccgatcccaatgccgccagccagttgttgctgcaacatatgcagagcgccagcctggtcatcgatccaatccacgaccgcatcctgcatgccaatccggcctgctgcgccctgctcgactggccgctggatgaactgctcgcactgcgcgccagccgtctctgggcgcatgaccttggggcactggtcaccttcaccgaggaagtgcaggtgcgcggcaatgcctggcgcgacggcctcgggctgctgcgtcgcgacggcgagcgcctggaagtggaaatcgacggcagccgactggagcatgacggcgtcatactgctcggcctgctgatccagcagcgccgccgcctcgacgccctgcgcgggctggccgaagccgagcgctatcaacgtcagggtctgctcgaatggcagggcgtggaacgcatcttccgccagttcgagcggcagaatcagttgatcctcggcgccgctggcgagggcatctacggcgtcaaccgtaatggcgagaccaccttcgtcaacccggcggccgagcgcattctcggctggcgtgccgacgatctgatcggccacaacattcacgacctgattcaccaccatcatccggacggcagcacctacgacggccacacctgcccgatctacgccgccttcaatgatggcgaagtgcatcaggtggccgacgaggtgttctggaacaaggacggcaagccgatcccggtggattacaccagcacgccgctgcgcgacgacggcgaactggtcggcgccgtggtggtgtttcgcgacatcagcgagcgcctggaaaccgagcgccggctgcgccaggctctgagcgaggtgcagcagctcaagcaacgcctggaactggagaatgcctacctacaggaagagattcgtggcgagtacgcccagcacgacctggtcgggcgcagcgccgccatgcagcaggtaatccaccagatcgagctggtcgcccccaccggcgccaacgtgctgatcaccggcgagtcaggcaccggcaaggagctgatcgcccgcgccattcacgacaacagcgggcgcagtcgtcgcccgctgatccgagtgaactgcgctgcggtgccgcgcgagctgttcgagagcgagttcttcggccatatccgcggcgccttcaccggcgcgctgaacgaccgcgtcgggcgcttcgaactggccgacggcggcacgctgtttctcgacgaggtcggcgaaattcctctggagctacagagcaagctgctgcgcgtgctacaggaacagcaactggaacgggtcggcgacacccgcacccgtcaggtcgacgtacgcgtgatcgccgccaccaaccgcgacctgcgccaggaagtacgcgccggccgcttccgcgaagacctgtactttcgcctcaacgtcttccccatcgagtcggcgccgctgcgtcagcgcccggaagacattccaccgctggcccagcactttctcggccgcgcctgtcgtcagctcaaccgccctgagccgagcctgcgcctggccgacatccaacgcctgcaagcctattcctggccgggcaacattcgtgagctggagaacctgatcgagcgtgcggtgatcatttcgcccggcacccggctgcgtctggatttgcccaacgacaacggcaacgacgcaccgtcacggccaccggtcgaacaggaaatgcggattttcactcagggtgaacaacgccggcagatgcgcgaaaacctgatcgcggcgctgacggcctgcgcaggcaggatatcggggaaggacggcgcggcgcggttgctggagctcaagcccaccaccctacgctcccgtctggagagcttcgccatcgacccgcgcgactaccgaccggggaggtcgcagccgcgcaggtacgagacgaatcagacgccgaggtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z