BBa_K944003 1 BBa_K944003 cioA gene related to cyanide insensitivity 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This came from the Pseudomonas aeruginosa genomic sequence we searched in genbank and synthesized by IDT Technologies. cioA encodes a hydrophobic protein of 488 amino acid residues with a predicted molecular mass of 54241 Da.cioA form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases. false false _1211_ 0 7118 9 It's complicated false This part has no restriction sites used in the protocol standard 10. false Natasha G??mez BBa_S05092 1 BBa_S05092 K944003:K944004 2012-09-25T11:00:00Z 2015-05-08T01:14:49Z false true _9_ 0 7118 9 Not in stock false false Natasha G??mez component2202249 1 BBa_K944004 component2202248 1 BBa_K944003 annotation2202248 1 BBa_K944003 range2202248 1 1 1467 annotation2202249 1 BBa_K944004 range2202249 1 1474 2481 BBa_K944004 1 BBa_K944004 cioB gene related to cyanide insensitivity 2012-09-25T11:00:00Z 2015-05-08T01:13:48Z This part came from the Pseudomonas aeruginosa genomic sequence and synthesized by IDT Technologies. cioB encodes a protein, which has the characteristics of a membrane protein, containing 335 amino acids with a predicted molecular mass of 3706 Da. cioB form part of the cioAB operon from Pseudomonas aeruginosa which code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases. false false _1211_ 0 7118 9 It's complicated false The cioB naturaly had a Pst1 restriction site in its sequence so we did a silent mutation for no longer to be this restriction site and proceed with the standard protocol 10. false Natasha G??mez BBa_S05092_sequence 1 atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactgatactagatgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactga BBa_K944004_sequence 1 atgggtatcgatcttcccctcatctgggcggtcatcatcatcttcggcatcatgatgtacgtggtgatggacggcttcgacctgggcatcggcatgctctatccgttcttcaaggacagcggcgaccgcgatgtgatgatgaacaccgtggcgccggtctgggacggcaacgagacctggctggtgctgggcggcgcggcgttgttcggggccttcccgctggcctacgcggtggtcctgtcggcgctctacctgccgttgatcttcatgctcgtcgggctgatcttccgcggcgtcgccttcgagttccgcttcaaggccaagccggccaagcagcacatctgggacaaggcgttcatcggcggttcgctggtggcgaccttcttccagggcgtggcgctgggcgccttcatcgaaggcatcccggtggagaaccgcagcttcgccggcggttcgctggactggatcgcgccgttcccgctgttctgcggcctggccctgatcgtcgcctacaccctgctcggctgcacctggctgatcatgaagaccgaaggccgcctacaggagcgcatgcacgacctggcgcgcccgctggcgttggtgctgctggcgatcatcggggtggtcagcctgtggacgccgctggcccatgccgagatcgccgcacgctggttcagcctgccgaacctgttctggttcctgccggtgccgatcctggtcctggtgaccttctatgcgctgctgcgctcggtggccaacaacgaccacgtgaagccgttcgtgctcaccctggtgttgatcttcctcggctacagcggcctggggatcagcctgtggccgaacatcatcccgccggcggtgagcatctgggaggcctcggcgccgccgcagagccagggcttcatgctggtcggcgcgctgttcatcattcccttcatcctcggctacaccgcctggagctactacgtgttccgcggcaaggtgaagcacggcgatggctatcactga BBa_K944003_sequence 1 atgttcggattggaggctatcgaccttgcccgcatccagttcgcgttcaccgtgtccttccacatcatcttcccggcgatcaccatcgggttggccagctacctggccgtactggaaggcctctggctgaaaacgaacgaagaggtctaccgcgacctctatcatttctggtcgaagattttcgcggtcaacttcggcatgggcgtggtgtccgggctggtcatggcgtaccagttcggcaccaactggagccatttctccgacttcgccgggtccatcaccggtccgctgctgacctacgaggtgctcaccgcgttcttcctcgaggccggtttcctcggggtcatgctgttcggctggaaccgcgtgggaccgggcctgcatttcttcgccacggtgatggtggcgatcggcacgctgatctcgacgttctggatcctcgcttccaacagctggatgcagaccccgcagggtttcgagatcgtcgacggccgggtgatcccggtggactggctggcggtgatcttcaacccgtcgttcccctaccgcctgctgcacatgtcggtcgccgcgttcctcgccaccgccttcttcgtcggcgcctcggccgcctggcacctgctgcgcgggcgcgacaacccggcgatccgcaagatgctctcgatggccatgtggatggcgctgatcgtcgccccggtacaggcgctgatcggcgacgcccatggcctcaataccctggagcaccagccggcgaagatcgccgcgatggaaggccactgggacaacagcagcggcgagccgaccccgctgatcctgttcggctggccggacatgcagcgcgaggaaacccgcttcaaggtggagatcccggtgctcggcagcctgatcctcaagcacagcctgaccgagccgatcccggcgctgaaggacttcccgccggaagaccggccgaactccaccgtggtgttctggtcgttccggatcatggccggcctcggcatgctgatgatcctggtcggcgtctggagcctgtggctgcgctggcgcggccaggacaagctgttcaactcgaaggccttcctgcgcctgaccctgctgatgggaccgtccggcctgatcgcgatcctcgccggctggttcaccaccgagatgggccgccagccctgggtggtctacggactgatgcgcaccgccgacgcctcctcggcgcagagcgtgacgcagatgagcctgaccctgctgaccttcgtggtggtgtacttctcgctgttcggcgtcggtatcggctacatgatgcgcctggtgcgcaagggcccggtcacccatgagggacgcgagaccaacccgggaggcgccgggcagaagcgcacgccggcacggccgttgtcggccgccggcgaaggtttcgacgaagagcacgacggccacgcgcaagcggccgaccgcgaccaggacaagaggaactga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z