LOCUS       BBa_S05115              2041 bp    DNA     linear       22-Dec-2024
DEFINITION  S05101:I13507
ACCESSION   BBa_S05115
VERSION     BBa_S05115.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    1109..1147
                     /label=LVA
     CDS             20..1147
                     /label=lacI-LVA
     misc_feature    20..1147
                     /label=BBa_C0012
     misc_feature    1148..1172
                     /label=Help:Barcodes
     CDS             20..1147
                     /label=BBa_C0012
     misc_feature    1..1172
                     /label=BBa_S05101
     misc_feature    1185..1188
                     /label=conserved
     RBS             1181..1192
                     /label=BBa_B0034
     CDS             1199..1873
                     /label=mrfp1
     misc_feature    1880..1904
                     /label=Help:Barcodes
     CDS             1199..1879
                     /label=BBa_E1010
     stem_loop       1924..1967
                     /label=stem_loop
     misc_feature    1913..1992
                     /label=BBa_B0010
     terminator      1913..1992
                     /label=BBa_B0010
     stem_loop       2008..2027
                     /label=T7 TE
     misc_feature    2034..2034
                     /label=stop
     polyA_site      2028..2041
                     /label=polya
     misc_feature    2001..2041
                     /label=BBa_B0012
     terminator      2001..2041
                     /label=BBa_B0012
     misc_feature    1181..2041
                     /label=BBa_I13507
ORIGIN
        1 tcacacagga aagtactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
       61 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
      121 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
      181 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
      241 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
      301 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
      361 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
      421 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
      481 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
      541 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
      601 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
      661 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
      721 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
      781 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
      841 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
      901 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
      961 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     1021 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1081 acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc
     1141 ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga aatactagat
     1201 ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc
     1261 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac
     1321 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct
     1381 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga
     1441 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga
     1501 cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa
     1561 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat
     1621 gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat
     1681 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta
     1741 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
     1801 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
     1861 ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat
     1921 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1981 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2041 a
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