##gff-version 3
##sequence-region BBa_S05115 1 2041
BBa_S05115	.	engineered_region	1181	2041	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_S05115	.	terminator	2001	2041	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_S05115	.	stem_loop	2008	2027	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05115	.	stop_codon	2034	2034	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05115	.	polyA_site	2028	2041	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05115	.	engineered_region	2001	2041	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S05115	.	ribosome_entry_site	1181	1192	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_S05115	.	conserved_region	1185	1188	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05115	.	terminator	1913	1992	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_S05115	.	stem_loop	1924	1967	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05115	.	engineered_region	1913	1992	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05115	.	CDS	1199	1879	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_S05115	.	CDS	1199	1873	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S05115	.	engineered_tag	1880	1904	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S05115	.	engineered_region	1	1172	.	+	0	ID=BBa_S05101;Name=BBa_S05101
BBa_S05115	.	CDS	20	1147	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S05101
BBa_S05115	.	CDS	20	1147	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S05115	.	engineered_tag	1148	1172	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S05115	.	engineered_region	20	1147	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S05115	.	tag	1109	1147	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S05115	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05101
BBa_S05115	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S05115	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S05115	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
>BBa_S05115
tcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttat
cagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgg
agctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccag
tctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtg
tcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctga
tcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttga
tgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtc
gcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggc
ataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaaca
aaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatg
cgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcat
gttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaact
ctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaat
acgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggc
aggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaaga
ggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgtt
aacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagtta
ccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaaca
cccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagac
ggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacca
acttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaaga
cggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacc
tacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaag
actacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtg
tagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata