LOCUS       BBa_S05116              1573 bp    DNA     linear       29-Jun-2024
DEFINITION  S05102:I13507
ACCESSION   BBa_S05116
VERSION     BBa_S05116.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             23..639
                     /label=tetR
     misc_feature    640..673
                     /label=SsrA
     misc_feature    680..704
                     /label=Help:Barcodes
     CDS             20..704
                     /label=BBa_C0040
     misc_feature    1..704
                     /label=BBa_S05102
     misc_feature    717..720
                     /label=conserved
     RBS             713..724
                     /label=BBa_B0034
     CDS             731..1405
                     /label=mrfp1
     misc_feature    1412..1436
                     /label=Help:Barcodes
     CDS             731..1411
                     /label=BBa_E1010
     stem_loop       1456..1499
                     /label=stem_loop
     misc_feature    1445..1524
                     /label=BBa_B0010
     terminator      1445..1524
                     /label=BBa_B0010
     stem_loop       1540..1559
                     /label=T7 TE
     misc_feature    1566..1566
                     /label=stop
     polyA_site      1560..1573
                     /label=polya
     misc_feature    1533..1573
                     /label=BBa_B0012
     terminator      1533..1573
                     /label=BBa_B0012
     misc_feature    713..1573
                     /label=BBa_I13507
ORIGIN
        1 tcacacagga aagtactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt
       61 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
      121 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
      181 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
      241 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
      301 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
      361 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
      421 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
      481 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
      541 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
      601 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta
      661 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agaaagagga
      721 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      781 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      841 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
      901 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
      961 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
     1021 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
     1081 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
     1141 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
     1201 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
     1261 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
     1321 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
     1381 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact
     1441 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1501 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1561 ttctgcgttt ata
//