##gff-version 3
##sequence-region BBa_S05116 1 1573
BBa_S05116	.	engineered_region	713	1573	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_S05116	.	terminator	1533	1573	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_S05116	.	stop_codon	1566	1566	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05116	.	engineered_region	1533	1573	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S05116	.	stem_loop	1540	1559	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05116	.	polyA_site	1560	1573	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05116	.	CDS	731	1411	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_S05116	.	engineered_tag	1412	1436	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S05116	.	CDS	731	1405	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S05116	.	ribosome_entry_site	713	724	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_S05116	.	conserved_region	717	720	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05116	.	terminator	1445	1524	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_S05116	.	stem_loop	1456	1499	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05116	.	engineered_region	1445	1524	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05116	.	engineered_region	1	704	.	+	0	ID=BBa_S05102;Name=BBa_S05102
BBa_S05116	.	CDS	20	704	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S05102
BBa_S05116	.	engineered_tag	680	704	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S05116	.	tag	640	673	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S05116	.	CDS	23	639	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S05116	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05102
BBa_S05116	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S05116	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S05116	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_S05116
tcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtc
ggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaa
ataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggga
aagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagta
catttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttttt
cactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatca
agtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgat
caccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaa
gtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactaga
gaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggt
tccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactga
aagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgt
taaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttc
gaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtg
gtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtaccc
ggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa
accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccaca
acgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtg
ctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata