LOCUS       BBa_S05121              2049 bp    DNA     linear       05-Nov-2024
DEFINITION  S05101:J06702
ACCESSION   BBa_S05121
VERSION     BBa_S05121.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    1109..1147
                     /label=LVA
     CDS             20..1147
                     /label=lacI-LVA
     misc_feature    20..1147
                     /label=BBa_C0012
     misc_feature    1148..1172
                     /label=Help:Barcodes
     CDS             20..1147
                     /label=BBa_C0012
     misc_feature    1..1172
                     /label=BBa_S05101
     misc_feature    1185..1188
                     /label=conserved
     RBS             1181..1192
                     /label=BBa_B0034
     CDS             1199..1909
                     /label=mCherry
     misc_feature    1550..1550
                     /label=C->T (removing PstI site)
     misc_feature    1199..1912
                     /label=BBa_J06504
     stem_loop       1932..1975
                     /label=stem_loop
     misc_feature    1921..2000
                     /label=BBa_B0010
     terminator      1921..2000
                     /label=BBa_B0010
     stem_loop       2016..2035
                     /label=T7 TE
     misc_feature    2042..2042
                     /label=stop
     polyA_site      2036..2049
                     /label=polya
     misc_feature    2009..2049
                     /label=BBa_B0012
     terminator      2009..2049
                     /label=BBa_B0012
     misc_feature    1181..2049
                     /label=BBa_J06702
ORIGIN
        1 tcacacagga aagtactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
       61 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
      121 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
      181 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
      241 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
      301 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
      361 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
      421 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
      481 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
      541 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
      601 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
      661 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
      721 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
      781 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
      841 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
      901 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
      961 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     1021 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1081 acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc
     1141 ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga aatactagat
     1201 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     1261 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1321 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1381 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1441 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1501 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctcct tgcaggacgg
     1561 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1621 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1681 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1741 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1801 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1861 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaat aatactagag
     1921 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1981 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     2041 gcgtttata
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