##gff-version 3
##sequence-region BBa_S05121 1 2049
BBa_S05121	.	engineered_region	1181	2049	.	+	0	ID=BBa_J06702;Name=BBa_J06702
BBa_S05121	.	terminator	1921	2000	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_S05121	.	engineered_region	1921	2000	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05121	.	stem_loop	1932	1975	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05121	.	engineered_region	1199	1912	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_S05121	.	sequence_alteration	1550	1550	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_S05121	.	CDS	1199	1909	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_S05121	.	terminator	2009	2049	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_S05121	.	stem_loop	2016	2035	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05121	.	polyA_site	2036	2049	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05121	.	stop_codon	2042	2042	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05121	.	engineered_region	2009	2049	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S05121	.	ribosome_entry_site	1181	1192	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_S05121	.	conserved_region	1185	1188	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05121	.	engineered_region	1	1172	.	+	0	ID=BBa_S05101;Name=BBa_S05101
BBa_S05121	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05101
BBa_S05121	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S05121	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S05121	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S05121	.	CDS	20	1147	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S05101
BBa_S05121	.	engineered_tag	1148	1172	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S05121	.	engineered_region	20	1147	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S05121	.	tag	1109	1147	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S05121	.	CDS	20	1147	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
>BBa_S05121
tcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttat
cagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgg
agctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccag
tctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtg
tcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctga
tcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttga
tgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtc
gcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggc
ataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaaca
aaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatg
cgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcat
gttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaact
ctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaat
acgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggc
aggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaaga
ggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcac
atggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccg
ccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaa
ggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtg
atgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtga
agctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcg
gatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgct
gaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatca
cctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacga
gctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata