LOCUS       BBa_S05124              1580 bp    DNA     linear       05-Nov-2024
DEFINITION  S05105:J06702
ACCESSION   BBa_S05124
VERSION     BBa_S05124.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=BBa_J61117
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..703
                     /label=BBa_C0040
     misc_feature    1..703
                     /label=BBa_S05105
     misc_feature    716..719
                     /label=conserved
     RBS             712..723
                     /label=BBa_B0034
     CDS             730..1440
                     /label=mCherry
     misc_feature    1081..1081
                     /label=C->T (removing PstI site)
     misc_feature    730..1443
                     /label=BBa_J06504
     stem_loop       1463..1506
                     /label=stem_loop
     misc_feature    1452..1531
                     /label=BBa_B0010
     terminator      1452..1531
                     /label=BBa_B0010
     stem_loop       1547..1566
                     /label=T7 TE
     misc_feature    1573..1573
                     /label=stop
     polyA_site      1567..1580
                     /label=polya
     misc_feature    1540..1580
                     /label=BBa_B0012
     terminator      1540..1580
                     /label=BBa_B0012
     misc_feature    712..1580
                     /label=BBa_J06702
ORIGIN
        1 aaagacatga gttactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag
      721 aaatactaga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      781 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      841 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      901 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      961 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1021 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1081 ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1141 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     1201 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     1261 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     1321 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     1381 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     1441 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1501 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1561 tgggcctttc tgcgtttata
//