##gff-version 3
##sequence-region BBa_S05124 1 1580
BBa_S05124	.	engineered_region	712	1580	.	+	0	ID=BBa_J06702;Name=BBa_J06702
BBa_S05124	.	terminator	1452	1531	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_S05124	.	stem_loop	1463	1506	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S05124	.	engineered_region	1452	1531	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S05124	.	engineered_region	730	1443	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_S05124	.	sequence_alteration	1081	1081	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_S05124	.	CDS	730	1440	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_S05124	.	ribosome_entry_site	712	723	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_S05124	.	conserved_region	716	719	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S05124	.	terminator	1540	1580	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_S05124	.	polyA_site	1567	1580	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S05124	.	engineered_region	1540	1580	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S05124	.	stem_loop	1547	1566	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S05124	.	stop_codon	1573	1573	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S05124	.	engineered_region	1	703	.	+	0	ID=BBa_S05105;Name=BBa_S05105
BBa_S05124	.	CDS	19	703	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S05105
BBa_S05124	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S05124	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S05124	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S05124	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_J61117;Name=BBa_J61117;Parent=BBa_S05105
>BBa_S05124
aaagacatgagttactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaagg
tgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccca
gaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggc
tccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagc
gcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaa
ggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctcc
gagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacg
acgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgga
catcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatg
gacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata