LOCUS       BBa_S05140              2957 bp    DNA     linear       26-Jun-2024
DEFINITION  B0032:S05137
ACCESSION   BBa_S05140
VERSION     BBa_S05140.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..2086
                     /label=BBa_K986000
     RBS             2095..2107
                     /label=RBS-3\Weak
     RBS             2101..2104
                     /label=RBS
     misc_feature    2095..2107
                     /label=BBa_B0032
     RBS             2095..2107
                     /label=BBa_B0032
     misc_feature    2114..2833
                     /label=GFP
     misc_feature    2834..2866
                     /label=LVA
     CDS             2114..2869
                     /label=BBa_K082003
     misc_feature    2095..2869
                     /label=BBa_S05100
     stem_loop       2889..2932
                     /label=stem_loop
     misc_feature    2878..2957
                     /label=BBa_B0010
     terminator      2878..2957
                     /label=BBa_B0010
     misc_feature    2095..2957
                     /label=BBa_K986001
     misc_feature    20..2957
                     /label=BBa_S05137
ORIGIN
        1 tcacacagga aagtactaga tgggtcagga aaagctatac atcgaaaaag agctcagttg
       61 gttatcgttc aatgaacgcg tgcttcagga agcggcggac aaatctaacc cgctgattga
      121 aaggatgcgt ttcctgggga tctattccaa taaccttgat gagttctata aagtccgctt
      181 cgctgaactg aagcgacgca tcattattag cgaagaacaa ggctccaact ctcattcccg
      241 ccatttactg ggcaaaattc agtcccgggt gctgaaagcc gatcaggagt ttgacggcct
      301 ctacaacgag ctattgctgg agatggcgcg caaccagatc ttcctgatta atgaacgcca
      361 gctctccgtc aatcaacaaa actggctgcg tcattatttt aagcagtatc tgcgtcagca
      421 cattacgccg attttaatca atcctgacac tgacttagtg cagttcctga aagatgatta
      481 cacctatctg gcggtggaaa ttatccgtgg cgataccatc cgttacgcgc tgctggagat
      541 cccatcagat aaagtgccgc gctttgtgaa tttaccgcca gaagcgccgc gtcgacgcaa
      601 gccgatgatt cttctggata acattctgcg ttactgcctt gatgatattt tcaaaggctt
      661 ctttgattat gacgcgctga atgcctattc aatgaagatg acccgcgatg ccgaatacga
      721 tttagtgcat gagatggaag ccagcctgat ggagttgatg tcttccagtc tcaagcagcg
      781 tttaactgct gagccggtgc gttttgttta tcagcgcgat atgcccaatg cgctggttga
      841 agtgttacgc gaaaaactga ctatttcccg ctacgactcc atcgtccccg gcggtcgtta
      901 tcataatttt aaagacttta ttaatttccc caatgtcggc aaagccaatc tggtgaacaa
      961 accactgccg cgtttacgcc atatttggtt tgataaagcc cagttccgca atggttttga
     1021 tgccattcgc gaacgcgatg tgttgctcta ttatccttat cacacctttg agcatgtgct
     1081 ggaactgctg cgtcaggctt cgttcgaccc gagcgtactg gcgattaaaa ttaacattta
     1141 ccgcgtggcg aaagattcac gcatcatcga ctcgatgatc cacgccgcac ataacggtaa
     1201 gaaagtcacc gtggtggttg agttacaggc gcgtttcgac gaagaagcca acattcactg
     1261 ggcgaagcgc ctgaccgaag caggcgtgca cgttatcttc tctgcgccgg ggctgaaaat
     1321 tcacgccaaa ctgttcctga tttcacgtaa agaaaacggt gaagtggtgc gttatgcaca
     1381 catcgggacc gggaacttta acgaaaaaac cgcgcgtctt tatactgact attcgttgct
     1441 gaccgccgat gcgcgcatca ccaacgaagt acggcgggta tttaacttta ttgaaaaccc
     1501 ataccgtccg gtgacatttg attatttaat ggtatcgccg caaaactccc gccgcctatt
     1561 gtatgaaatg gtggaccgcg agatcgccaa cgcgcagcaa gggctgccca gtggtatcac
     1621 cctgaagcta aataaccttg tcgataaagg cctggttgat cgtctgtatg cggcctccag
     1681 ctccggcgta ccggttaatc tgctggttcg cggaatgtgt tcgctgatcc ccaatctgga
     1741 aggcattagc gacaacattc gtgccatcag tattgttgac cgttaccttg aacatgaccg
     1801 ggtttatatt tttgaaaatg gcggcgataa aaaggtctac ctttcttccg ccgactggat
     1861 gacgcgcaat attgattatc gtattgaagt ggcgacgccg ctgctcgatc cgcgcctgaa
     1921 gcagcgggta ctggacatca tcgacatatt gttcagcgat acggtcaaag cacgttatat
     1981 cgataaagaa ctcagtaatc gctacgttcc ccgcggcaat cgccgcaaag tacgggcgca
     2041 gttggcgatt tatgactaca tcaaatcact cgaacaacct gaataatact agagtcacac
     2101 aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     2161 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     2221 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     2281 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     2341 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     2401 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     2461 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     2521 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     2581 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     2641 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     2701 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     2761 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     2821 atacaaaagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat actagagcca
     2881 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2941 tgtcggtgaa cgctctc
//