LOCUS BBa_S05142 7083 bp DNA linear 29-Dec-2025 DEFINITION M30109:K990001 ACCESSION BBa_S05142 VERSION BBa_S05142.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 misc_feature 883..907 /label=Help:Barcodes CDS 157..882 /label=BBa_I15008 misc_feature 920..923 /label=conserved RBS 916..927 /label=BBa_B0034 CDS 934..1683 /label=PcyA misc_feature 1684..1708 /label=Help:Barcodes CDS 934..1683 /label=BBa_I15009 stem_loop 1728..1771 /label=stem_loop misc_feature 1717..1796 /label=BBa_B0010 terminator 1717..1796 /label=BBa_B0010 stem_loop 1812..1831 /label=T7 TE misc_feature 1838..1838 /label=stop polyA_site 1832..1845 /label=polya misc_feature 1805..1845 /label=BBa_B0012 terminator 1805..1845 /label=BBa_B0012 misc_feature 1854..1872 /label=TetR 1 misc_feature 1854..1907 /label=BBa_R0040 promoter 1873..1878 /label=-35 misc_feature 1879..1897 /label=TetR 2 promoter 1896..1901 /label=-10 promoter 1854..1907 /label=BBa_R0040 misc_feature 1920..1923 /label=conserved RBS 1916..1927 /label=BBa_B0034 CDS 1934..4171 /label=Cph8 misc_feature 4172..4196 /label=Help:Barcodes CDS 1934..4171 /label=BBa_I15010 stem_loop 4216..4259 /label=stem_loop misc_feature 4205..4284 /label=BBa_B0010 terminator 4205..4284 /label=BBa_B0010 stem_loop 4300..4319 /label=T7 TE misc_feature 4326..4326 /label=stop polyA_site 4320..4333 /label=polya misc_feature 4293..4333 /label=BBa_B0012 terminator 4293..4333 /label=BBa_B0012 misc_feature 1..4333 /label=BBa_M30109 misc_feature 4354..4371 /label=C1 OmpR misc_feature 4375..4392 /label=C2 OmpR misc_feature 4395..4412 /label=C3 OmpR promoter 4416..4421 /label=-35 promoter 4439..4444 /label=-10 promoter 4342..4449 /label=BBa_R0082 RBS 4458..4474 /label=rbs CDS 4475..5398 /label=Luciferin regenerating enzyme (P.pyralis) misc_feature 5399..5404 /label=double stop codon misc_feature 5405..5410 /label=BamH1 site RBS 5411..5427 /label=rbs misc_feature 5428..7077 /label=EPIC luciferase (P. pyralis) misc_feature 7078..7083 /label=double stop codon misc_feature 4458..7083 /label=BBa_K325100 misc_feature 4342..7083 /label=BBa_K990001 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga gtgtcaactt agcttcccag 181 ttgcgggaag ggacgaaaaa atcccactcc atggcggaga acgtcggctt tgtcaaatgc 241 ttcctcaagg gcgttgtcga gaaaaattcc taccgtaagc tggttggcaa tctctacttt 301 gtctacagtg ccatggaaga ggaaatggca aaatttaagg accatcccat cctcagccac 361 atttacttcc ccgaactcaa ccgcaaacaa agcctagagc aagacctgca attctattac 421 ggctccaact ggcggcaaga agtgaaaatt tctgccgctg gccaagccta tgtggaccga 481 gtccggcaag tggccgctac ggcccctgaa ttgttggtgg cccattccta cacccgttac 541 ctgggggatc tttccggcgg tcaaattctc aagaaaattg cccaaaatgc catgaatctc 601 cacgatggtg gcacagcttt ctatgaattt gccgacattg atgacgaaaa ggcttttaaa 661 aatacctacc gtcaagctat gaatgatctg cccattgacc aagccaccgc cgaacggatt 721 gtggatgaag ccaatgacgc ctttgccatg aacatgaaaa tgttcaacga acttgaaggc 781 aacctgatca aggcgatcgg cattatggtg ttcaacagcc tcacccgtcg ccgcagtcaa 841 ggcagcaccg aagttggcct cgccacctcc gaaggctaat aacgctgata gtgctagtgt 901 agatcgctac tagagaaaga ggagaaatac tagatggccg tcactgattt aagtttgacc 961 aattcttccc tgatgcctac gttgaacccg atgattcaac agttggccct ggcgatcgcc 1021 gctagttggc aaagtttacc cctcaagccc tatcaattgc cggaggattt gggctacgta 1081 gaaggccgcc tggaagggga aaagttagtg attgaaaatc ggtgctacca aacgccccag 1141 tttcgcaaaa tgcatttgga gttggccaag gtgggcaaag ggttggatat tctccactgt 1201 gtaatgtttc ctgagccttt atacggtcta cctttgtttg gctgtgacat tgtggccggc 1261 cccggtggag taagtgcggc tattgcggat ctatccccca cccaaagcga tcgccaattg 1321 cccgcagcgt accaaaaatc attggcagag ctaggccagc cagaatttga gcaacaacgg 1381 gaattgcccc cctggggaga aatattttct gaatattgtt tattcatccg tcccagcaat 1441 gtcactgaag aagaaagatt tgtacaaagg gtagtggact ttttgcaaat tcattgtcac 1501 caatccatcg ttgccgaacc cttgtctgaa gctcaaactt tggagcaccg tcaggggcaa 1561 attcattact gccaacaaca acagaaaaat gataaaaccc gtcgggtact ggaaaaagct 1621 tttggggaag cttgggcgga acggtatatg agccaagtct tatttgatgt tatccaataa 1681 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg 1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat 1861 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga 1921 ggagaaatac tagatggcca ccaccgtaca actcagcgac caatccctcc gtcagctaga 1981 aaccctcgcc atccacaccg cccacctgat tcagccccac ggtttagtgg tggtcctgca 2041 agaaccagac ctcaccatca gccaaattag cgccaactgc accggcattt tagggcgatc 2101 gccagaggat ttgttgggca gaaccctagg ggaagtgttt gatagctttc agattgatcc 2161 catccagagt cgcctaacgg ccggacaaat cagcagcctc aaccccagta aactttgggc 2221 gcgggtcatg ggggacgact ttgtcatttt tgacggggtt tttcatcgca acagtgacgg 2281 tttattggta tgtgaactcg agccagccta cacttccgat aatctgccct tcctcggttt 2341 ttatcacatg gccaacgctg ccctgaatcg gttgcgccaa caagctaatc tacgggattt 2401 ctacgatgtt attgtcgaag aagtccgccg tatgactggc tttgaccggg tgatgctata 2461 ccgctttgat gaaaataacc acggtgatgt cattgccgaa gataaacggg atgatatgga 2521 accctatttg ggcctgcact atcccgaatc ggatattccc caacccgccc gtcggctatt 2581 tatccacaac cccattcgag taattcccga tgtttatggt gtggcggtgc ccctgacccc 2641 agcggttaac cccagcacca accgagcggt ggatttaaca gaatccattc tgcgcagtgc 2701 gtaccattgc cacttgacct atctgaaaaa tatgggggta ggagcgtctt taaccatttc 2761 cctaattaag gacggccatc tctgggggct cattgcctgc caccatcaaa cccccaaagt 2821 aattcccttt gaactgcgta aagcctgcga attttttggt cgggtggtgt ttagcaacat 2881 ttccgcccag gaagatacgg aaaccttcga ttaccgggtg cagctggcgg agcatgaagc 2941 ggttttattg gacaaaatga ccacggcggc ggattttgtc gaaggattaa ctaatcatcc 3001 cgatcgcctg ttgggattaa cgggctccca gggggcggcc atttgctttg gggaaaaatt 3061 gattttagta ggggaaaccc cggacgagaa agcagtgcaa tatttactgc aatggttgga 3121 gaatcgggaa gtgcaagacg ttttcttcac ctcttccctc tcacaaattt atcctgatgc 3181 agtgaatttt aaatccgtgg ccagtggctt attggccatt cccattgccc gtcacaactt 3241 tttgctctgg tttcgccctg aagtgttgca aacggttaat tggggcggtg acccaaatca 3301 tgcttacgaa gctacccagg aagacggtaa aatcgagctc catccccgcc aatcctttga 3361 cctctggaaa gaaattgtcc gactccaatc tttgccctgg caatcggtgg aaatccaaag 3421 tgccctggcc ctgaaaaagg cgatcgtcaa cctcattttg cgccaggcag aagaattgca 3481 tatggcggct ggtgttaagc aactggcgga tgaccgcacg ctgctgatgg cgggggtaag 3541 tcacgacttg cgcacgccgc tgacgcgtat tcgcctggcg actgagatga tgagcgagca 3601 ggatggctat ctggcagaat cgatcaataa agatatcgaa gagtgcaacg ccatcattga 3661 gcagtttatc gactacctgc gcaccgggca ggagatgccg atggaaatgg cggatcttaa 3721 tgcagtactc ggtgaggtga ttgctgccga aagtggctat gagcgggaaa ttgaaaccgc 3781 gctttacccc ggcagcattg aagtgaaaat gcacccgctg tcgatcaaac gcgcggtggc 3841 gaatatggtg gtcaacgccg cccgttatgg caatggctgg gtcaaagtca gcagcggaac 3901 ggagccgaat cgcgcctggt tccaggtgga agatgacggt ccgggaattg cgccggaaca 3961 acgtaagcac ctgttccagc cgtttgtccg cggcgacagt gcgcgcacca ttagcggcac 4021 gggattaggg ctggcaattg tgcagcgtat cgtggataac cataacggga tgctggagct 4081 tggcaccagc gagcggggcg ggctttccat tcgcgcctgg ctgccagtgc cggtaacgcg 4141 ggcgcagggc atgacaaaag aagggtaata actctgatag tgctagtgta gatctctact 4201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 4261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 4321 ttctgcgttt atatactaga gtcccttgca tttacatttt gaaacatcta tagcgataaa 4381 tgaaacatct taaaagtttt agtatcatat tcgtgttgga ttattctgca tttttgggga 4441 gaatggactt actagagtta aggaggtaaa aaaaatgggt ccggtcgttg aaaagatcgc 4501 cgagctgggt aagtacacgg ttggtgaggg tccgcactgg gaccacgaaa cgcagacgct 4561 gtatttcgtg gatacggtcg agaaaacgtt ccacaaatac gttccgagcc agaagaaata 4621 cacgttttgt aaggtggaca agctggttag ctttatcatt ccgctggcag gttctccggg 4681 tcgcttcgtc gtcagcttgg agcgcgagat tgcgatcctg acgtgggacg gcgtcagcgc 4741 ggcaccgacc agcattgagg cgattgtgaa tgtcgagccg cacatcaaga ataatcgcct 4801 gaacgacggt aaagcggacc cgctgggtaa tctgtggacc ggcacgatgg ccattgatgc 4861 gggtttgccg gtgggtccgg tgaccggctc cctgtaccac ctgggtgcgg ataagaaggt 4921 caagatgcat gaaagcaata ttgccatcgc taacggcctg gcgtggagca acgacctgaa 4981 gaagatgtac tatattgaca gcggcaaacg tcgtgttgac gaatacgact atgatgcgag 5041 cacgctgtct atcagcaatc agcgtccgct gttcaccttc gaaaaacatg aggtccctgg 5101 ctacccagac ggtcaaacca ttgacgaaga aggtaacctg tgggtcgccg tgtttcaagg 5161 ccagcgtatt atcaagatta gcacccagca gccggaggtt ctgctggata ccgtcaaaat 5221 cccggacccg caggtgacta gcgttgcatt cggtggtccg aatctggacg agctgcatgt 5281 gaccagcgcc ggtttgcagc tggatgacag ctccctggac aaatctttgg tcaatggtca 5341 cgtctaccgt gtgactggcc tgggtgttaa gggtttcgcc ggtgtgaaag ttaagctgta 5401 ataaggatcc ttaaggaggt aaaaaaaatg gaggacgcga aaaacatcaa gaaaggccca 5461 gcaccgttct atccgctgga agatggtacc gccggtgaac aattgcacaa agcgatgaaa 5521 cgttatgcgc tggttccggg taccatcgca ttcacggacg cgcatatcga ggttaacatc 5581 acctatgccg agtacttcga gatgagcgtt cgtctggccg aggcaatgaa gcgttatggt 5641 ttgaatacca atcatcgcat cgtcgtctgt agcgagaaca gcttgcagtt tttcatgcca 5701 gttctgggtg cactgtttat tggtgtggca gtggcgcctg cgaatgatat ctacaacgag 5761 cgtgaactgt tgaatagcat gaacatttcg cagccgacgg ttgtttttgt gtccaagaaa 5821 ggtttgcaga agattctgaa tgtgcaaaag aagttgccga tcattcagaa aatcatcatc 5881 atggacagca agaccgatta ccagggtttc caaagcatgt acacttttgt gacgagccac 5941 ctgcctccgg gttttaacga gtatgatttc gttccggaaa gcttcgaccg tgataagacg 6001 attgcactga ttatgaactc gtccggctct accggcctgc ctaaaggcgt ggccctgccg 6061 caccgtactg catgtgtccg cttcagccac gcgcgtgatc cgatcttcgg taaccaaatc 6121 attccggaca ccgccatttt gtctgtcgtt ccgtttcatc atggctttgg tatgtttacc 6181 actctgggtt acctgatctg tggtttccgt gttgttctga tgtaccgttt cgaagaagaa 6241 ctgttcctgc gtagcctgca agattacaag atccaatccg cgctgctggt gccgacgctg 6301 tttagcttct tcgctaaatc caccctgatt gacaaatatg acttgagcaa cctgcacgaa 6361 atcgcgtcgg gcggtgcgcc gctgagcaaa gaagtcggtg aggccgttgc gaaacgtttc 6421 catctgccgg gcatccgtca gggttatggc ctgacggaaa cgaccagcgc gattttgatt 6481 acccctgagg gtgacgataa accgggtgcc gttggcaagg tggtaccgtt ctttgaggct 6541 aaagtcgtgg acctggacac gggtaagact ctgggtgtga atcaacgtgg cgagttgtgc 6601 gttcgtggcc cgatgatcat gagcggttat gtcaacaacc cggaagcaac taatgcgttg 6661 atcgataagg atggctggct gcacagcggc gacctggcgt actgggatga ggatgaacac 6721 tttttcatcg ttggtcgcct gaagagcctg atcaagtaca agggctacca ggtggcaccg 6781 gctgaactgg agagcattct gttgcaacac cctaatatct tcgacgcggg tgttgcgggt 6841 ctgcctgatg atgatgccgg tgaattgcca gctgcggtgg tcgttctgga acacggtaaa 6901 acgatgactg aaaaagaaat cgtggactac gtcgcctcgc aggtgaccac cgctaaaaag 6961 ttgcgtggtg gcgtggtgtt tgtggatgaa gtgccgaaag gcctgacggg taaacgtgat 7021 gcacgtaaga ttcgtgagat cttgatcaag gcgaagaaag gtggtaaatc caagttgtaa 7081 taa //