BBa_S05201
1
BBa_S05201
K1051302:K1051201
2013-09-09T11:00:00Z
2015-05-08T01:14:51Z
false
false
_9_
0
15920
9
Not in stock
false
false
Yang Zhou
component2367142
1
BBa_K1051201
component2367141
1
BBa_K1051303
annotation2367142
1
BBa_K1051201
range2367142
1
708
746
annotation2367141
1
BBa_K1051303
range2367141
1
1
699
BBa_K1051303
1
BBa_K1051303
clb6 promoter (during G1 phase)
2013-06-29T11:00:00Z
2015-05-08T01:08:54Z
TAACT AGGTG GTCCT TCCAT GAAAGTTTTTTTTTTTTGAAGATGTTCTTTTTTTTTTTTT
GGGCCTCATTACAATGTAAGAGGTTATTATGTGTTACATGAGTAAAGCTAATTGACGCAA
GCAGGTAATGAAGAAAGCCCTATATTACCGTATAGACAATGGGCGCGTCCCTAAAGAGGA
TACCAAATAGCAGTTTTACGCGTACCACAAAATTTAACGACCAACGCGTATTCTTCGCGT
TTTTTTTTTGCGACGGTAAACAAAAAAAGGGCCCTCTGCGGTAAAACAAGTGGGTCTACC
ACAGGACACAGGACTTGGTTGTTTCTACTCTCCCTGTGTATCCTACAATTGGCAATTGGC
AATTCGCAACCTGCGTTTAAATATTTACCGTTTTAGCACATTGATTACCTTTTTTGCTAG
GGTTAAGTTTTTGCCTTTTTCTTATTAACATATATATAACTGCTTCATTTCATACAAGTG
ACTTTTATCTTTTATTAAATAATAACAAGCTTCACTAATCTAGCGTTCATACAACATACA
AATCAGTGTGTTGAAAATTATTATTCTCTGATATTCTCTCCCTCCTTTTAAATTTTTAAA
It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb6 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .
false
false
_1358_
0
15940
9
It's complicated
true
We get the sequence which is actived by clb6 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows :
upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTAACT AGGTG GTCCT TCCAT
downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTAAAAATTTAAAAGGAGG
false
Kequan Lin
BBa_K1051201
1
BBa_K1051201
D-BOX
2013-06-29T11:00:00Z
2015-05-08T01:08:54Z
5' - AATTC GCGGCCGC T TCTAGA GTACAAAGGTTGGCCTTAAACAATGTAACAAATACGACA ACTAGT A GCGGCCG CTGCA- 3'
5???- G CGGCCGC T ACTAGT TGTCGTATTTGTTACATTGTTTAAGGCCAACCTTTGTAC TCTAGAA GCGGCCGC G-3???
D-BOX
false
false
_1358_
0
18073
9
In stock
false
D-BOX
false
wanling ZHOU
BBa_S05201_sequence
1
acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaatactagaggtacaaaggttggccttaaacaatgtaacaaatacgaca
BBa_K1051303_sequence
1
acaacactttcagaaaatcaagctcgtatggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttttattaactaggtggtccttccatgaaagttttttttttttgaagatgttctttttttttttttgggcctcattacaatgtaagaggttattatgtgttacatgagtaaagctaattgacgcaagcaggtaatgaagaaagccctatattaccgtatagacaatgggcgcgtccctaaagaggataccaaatagcagttttacgcgtaccacaaaatttaacgaccaacgcgtattcttcgcgttttttttttgcgacggtaaacaaaaaaagggccctctgcggtaaaacaagtgggtctaccacaggacacaggacttggttgtttctactctccctgtgtatcctacaattggcaattggcaattcgcaacctgcgtttaaatatttaccgttttagcacattgattaccttttttgctagggttaagtttttgcctttttcttattaacatatatataactgcttcatttcatacaagtgacttttatcttttattaaataataacaagcttcactaatctagcgttcatacaacatacaaatcagtgtgttgaaaattattattctctgatattctctccctccttttaaatttttaaa
BBa_K1051201_sequence
1
gtacaaaggttggccttaaacaatgtaacaaatacgaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z