LOCUS       BBa_S05214              2547 bp    DNA     linear       01-Jun-2024
DEFINITION  K1149022:K1149030
ACCESSION   BBa_S05214
VERSION     BBa_S05214.1  
FEATURES             Location/Qualifiers
     misc_feature    1..66
                     /label=PelB
     misc_feature    1..66
                     /label=BBa_J32015
     misc_feature    1776..1803
                     /label=6his3stop
     CDS             67..1803
                     /label=BBa_K1149030
     RBS             1804..1827
                     /label=rbs
     CDS             1828..2547
                     /label=sfGFP
     misc_feature    1804..2547
                     /label=BBa_K515005
ORIGIN
        1 atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg
       61 atggccatga tgcgagccga gttgcatcgc tctccccgac aacaccgtct cggcaaacaa
      121 cgacaacatg aggaggatga cccagtgcga accaaattcg gcgcctggaa gccgctgacc
      181 gcgttggcat cggcgatcct gttggccgcc tctgtggctg gctgtggctc aagttcgaaa
      241 tcggacgaca gggaaccccc accggctccg agaatgggca actatcaggg aaccgacgaa
      301 ggcagcatgc ttgccttccg cggcattcgc tacgccaagc cgcccgtagg cgaactgcgg
      361 ttcgcgccac cccagccggt cgagaagcca acggggattg tggtcgccga taaattcggc
      421 agcgcctgtc cccagtacca aagcgccttc ggcccggaaa gcctcgaaga ggactgcctt
      481 ttcctcaacg tgtacgtccc caaggctggc gagggcccct tcccggtcat ggtctggatc
      541 catggcggcg cattcgttgc tggttcaggc ggtccagaat atgacccctc acgcttggta
      601 gcccagggtg tggtggtcgt cgccatcaac tatcgcctgg gcccgctcgg ctttctcgcc
      661 catccggatt taaccaacga acaaggcggc ggttcgggca actatggcct gatggatcag
      721 cagttagcgc tgcgctgggt ccaggacaat atctcaacct ttggcggcga tccaaacaac
      781 gtcactatct ttggtgaatc agccggcggc cttagcgtgc tgtcgcatgt cgtatcgcct
      841 aaggcacagg gtctcttcca gcgcgctatc gtccaaagcg gctcgtacga tgcaatcagc
      901 cgcaccttgg ctgaggccga gcaagcaggt caggatttcg tagctgccgt gggttgcgac
      961 tcggcagggg atattccgca atgcctgcgt gaactggatg tcaagaccat tctcgacaat
     1021 ggcgatgcaa tcgtagccgg cctctcgctg acgccccatt tacaccccga cctgctgccg
     1081 aaatcaatta accaggcttt gcaagaaggc aaagttaacg ttaattctat tctcctcggt
     1141 agcaatcgcg acgagttcac gttattcata gcccttgaag aactcaatgg aactccccca
     1201 atggaggaag atgatgtcga agatcaatgg tctaaatttt ttgacccttt gacttccata
     1261 gtaagcactt actatcctgc taccgacttt gaaaacccca gccgtcgctt cgctgcaccg
     1321 ttgggtgaca gcgttttcag ctgtaccgcc ttgcggcaag cagaacagct aggcagcacc
     1381 atccccgtct atgtctacga gttcgctgac cgcgatgctc ccagcattct gcctaaggtc
     1441 agcttcgacc ttggcgctgc ccatgctttc gaaatccagt atgtactagg cagcgagcaa
     1501 gccctgcggg cacgcggagc gaatgattcg caaattgcgc tttccaacgc catggttcaa
     1561 tactggactg ctttcgcccg taccggcgat cccaatcaag gggaggcgga agcaatttgg
     1621 ccggagtttg gaagcaataa cggcaatatt cgctggctaa accccgccga acctgagaaa
     1681 acggtgatca cgagcgctga gttcgaggca gatcaccgtt gcgaaatttg ggataaaata
     1741 atattccaag ctcttaaaca agatccccaa aaatcacacc atcaccatca ccattaatag
     1801 tgaaaatcaa attaaggagg taagataatg cgtaaaggcg aagaactgtt cacgggcgta
     1861 gttccgattc tggtcgagct ggacggcgat gtgaacggtc ataagtttag cgttcgcggt
     1921 gaaggtgagg gcgacgcgac caacggcaaa ctgaccctga agttcatctg caccaccggt
     1981 aaactgccgg tgccttggcc gaccttggtg acgacgttga cgtatggcgt gcagtgtttt
     2041 gcgcgttatc cggaccacat gaaacaacac gatttcttca aatctgcgat gccggagggt
     2101 tacgtccagg agcgtaccat ttccttcaag gatgatggct actacaaaac tcgcgcagag
     2161 gttaagtttg aaggtgacac gctggtcaat cgtatcgaat tgaagggtat cgactttaaa
     2221 gaggatggta acattctggg ccataaactg gagtataact tcaacagcca taatgtttac
     2281 attacggcag acaagcaaaa gaacggcatc aaggccaatt tcaagattcg ccacaatgtt
     2341 gaggacggta gcgtccaact ggccgaccat taccagcaga acaccccaat tggtgacggt
     2401 ccggttttgc tgccggataa tcactatctg agcacccaaa gcgtgctgag caaagatccg
     2461 aacgaaaaac gtgatcacat ggtcctgctg gaatttgtga ccgctgcggg catcacccac
     2521 ggtatggacg agctgtataa gtaatga
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