LOCUS       BBa_S05216              1554 bp    DNA     linear       01-Jun-2024
DEFINITION  K1149036:K515005
ACCESSION   BBa_S05216
VERSION     BBa_S05216.1  
FEATURES             Location/Qualifiers
     misc_feature    1..66
                     /label=BBa_K1149022
     misc_feature    783..810
                     /label=6His+3stop
     CDS             67..810
                     /label=BBa_K1149036
     RBS             811..834
                     /label=rbs
     CDS             835..1554
                     /label=sfGFP
     misc_feature    811..1554
                     /label=BBa_K515005
ORIGIN
        1 atgaaatatc tgctgccgac cgcagcagcg ggtctgctgc tgctggcagc acagcctgca
       61 atggcaatgc tggttagcgc actggcactg gcagttctga gcgcagcaag cctgggtcgt
      121 gcagcaccga caccggaaag tgccgaagca catgaactgg aagcacgtgc aaccagcagc
      181 gcatgtccgc agtatgttct gattaatacc cgtggcaccg gtgaaccgca gggtcagagc
      241 gcaggttttc gtaccatgaa tagccagatt accgcagcac tgagcggtgg caccatttat
      301 aacaccgttt ataccgcaga ttttagccag aatagtgcag caggcaccgc agatattatt
      361 cgtcgtatta atagcggtct ggcagccaat ccgaatgttt gttatattct gcaaggttat
      421 agccagggtg cagcagcaac cgttgttgcc ctgcaacagc tgggcaccag cggtgcagcc
      481 tttaatgccg ttaaaggtgt ttttctgatt ggcaacccgg atcacaaaag cggtctgacc
      541 tgtaatgttg atagcaatgg tggtacaacc acccgtaatg ttaatggtct gagcgttgca
      601 tatcagggta gcgttccgag cggttgggtt agcaaaaccc tggatgtttg tgcatatggt
      661 gatggtgttt gtgataccgc acatggtttt ggtattaatg cacagcatct gagctatccg
      721 agcgatcagg gtgttcagac catgggttac aaatttgcag ttaataaact gggtggtagc
      781 gcacatcatc atcaccatca ctaataatga aaatcaaatt aaggaggtaa gataatgcgt
      841 aaaggcgaag aactgttcac gggcgtagtt ccgattctgg tcgagctgga cggcgatgtg
      901 aacggtcata agtttagcgt tcgcggtgaa ggtgagggcg acgcgaccaa cggcaaactg
      961 accctgaagt tcatctgcac caccggtaaa ctgccggtgc cttggccgac cttggtgacg
     1021 acgttgacgt atggcgtgca gtgttttgcg cgttatccgg accacatgaa acaacacgat
     1081 ttcttcaaat ctgcgatgcc ggagggttac gtccaggagc gtaccatttc cttcaaggat
     1141 gatggctact acaaaactcg cgcagaggtt aagtttgaag gtgacacgct ggtcaatcgt
     1201 atcgaattga agggtatcga ctttaaagag gatggtaaca ttctgggcca taaactggag
     1261 tataacttca acagccataa tgtttacatt acggcagaca agcaaaagaa cggcatcaag
     1321 gccaatttca agattcgcca caatgttgag gacggtagcg tccaactggc cgaccattac
     1381 cagcagaaca ccccaattgg tgacggtccg gttttgctgc cggataatca ctatctgagc
     1441 acccaaagcg tgctgagcaa agatccgaac gaaaaacgtg atcacatggt cctgctggaa
     1501 tttgtgaccg ctgcgggcat cacccacggt atggacgagc tgtataagta atga
//