LOCUS       BBa_S05217              1947 bp    DNA     linear       14-Jun-2024
DEFINITION  K1149023:K1149035
ACCESSION   BBa_S05217
VERSION     BBa_S05217.1  
FEATURES             Location/Qualifiers
     misc_feature    1..66
                     /label=BBa_K1149023
     misc_feature    1182..1203
                     /label=6his+stop
     CDS             67..1203
                     /label=BBa_K1149035
     RBS             1204..1227
                     /label=rbs
     CDS             1228..1947
                     /label=sfGFP
     misc_feature    1204..1947
                     /label=BBa_K515005
ORIGIN
        1 atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg
       61 atggccatgg caccggccgt tgaacagcgt tctgaagcag ctcctctgat tgaggcacgt
      121 ggtgaaatgg tagcaaacaa gtacatcgtg aagttcaagg agggttctgc tctgtctgct
      181 ctggatgctg ctatggaaaa gatctctggc aagcctgatc acgtctataa gaacgtgttc
      241 agcggtttcg cagcaactct ggacgagaac atggtccgtg tactgcgtgc tcatccagac
      301 gttgaataca tcgaacagga cgctgtggtt actatcaacg cggcacagac taacgcacct
      361 tggggtctgg cacgtatttc ttctacttcc ccgggtacgt ctacttacta ctacgacgag
      421 tctgccggtc aaggttcttg cgtttacgtg atcgatacgg gcatcgaggc ttctcatcct
      481 gagtttgaag gccgtgcaca aatggtgaag acctactact actcttcccg tgacggtaat
      541 ggtcacggta ctcattgcgc aggtactgtt ggtagccgta cctacggtgt tgctaagaaa
      601 acgcaactgt tcggcgttaa agtgctggac gacaacggtt ctggtcagta ctccaccatt
      661 atcgcgggta tggatttcgt agcgagcgat aaaaacaacc gcaactgccc gaaaggtgtt
      721 gtggcttctc tgtctctggg tggtggttac tcctcttctg ttaacagcgc agctgcacgt
      781 ctgcaatctt ccggtgtcat ggtcgcagta gcagctggta acaataacgc tgatgcacgc
      841 aactactctc ctgctagcga gccttctgtt tgcaccgtgg gtgcatctga tcgttatgat
      901 cgtcgtagct ccttcagcaa ctatggttcc gtcctggata tcttcggccc tggtacttct
      961 atcctgtcta cctggattgg cggtagcact cgttccattt ccggtacgag catggctact
     1021 ccacatgttg ctggtctggc agcatacctg atgaccctgg gtaagaccac tgctgcatcc
     1081 gcttgtcgtt acatcgcgga tactgcgaac aaaggcgatc tgtctaacat cccgttcggc
     1141 accgttaatc tgctggcata caacaactat caggctgtcg accatcatca tcatcatcat
     1201 tgaaaatcaa attaaggagg taagataatg cgtaaaggcg aagaactgtt cacgggcgta
     1261 gttccgattc tggtcgagct ggacggcgat gtgaacggtc ataagtttag cgttcgcggt
     1321 gaaggtgagg gcgacgcgac caacggcaaa ctgaccctga agttcatctg caccaccggt
     1381 aaactgccgg tgccttggcc gaccttggtg acgacgttga cgtatggcgt gcagtgtttt
     1441 gcgcgttatc cggaccacat gaaacaacac gatttcttca aatctgcgat gccggagggt
     1501 tacgtccagg agcgtaccat ttccttcaag gatgatggct actacaaaac tcgcgcagag
     1561 gttaagtttg aaggtgacac gctggtcaat cgtatcgaat tgaagggtat cgactttaaa
     1621 gaggatggta acattctggg ccataaactg gagtataact tcaacagcca taatgtttac
     1681 attacggcag acaagcaaaa gaacggcatc aaggccaatt tcaagattcg ccacaatgtt
     1741 gaggacggta gcgtccaact ggccgaccat taccagcaga acaccccaat tggtgacggt
     1801 ccggttttgc tgccggataa tcactatctg agcacccaaa gcgtgctgag caaagatccg
     1861 aacgaaaaac gtgatcacat ggtcctgctg gaatttgtga ccgctgcggg catcacccac
     1921 ggtatggacg agctgtataa gtaatga
//