LOCUS       BBa_S05223              2139 bp    DNA     linear       23-Dec-2024
DEFINITION  K1149023:K1149038
ACCESSION   BBa_S05223
VERSION     BBa_S05223.1  
FEATURES             Location/Qualifiers
     misc_feature    1..66
                     /label=BBa_K1149023
     misc_feature    1369..1395
                     /label=flag+2stop
     CDS             67..1395
                     /label=BBa_K1149038
     RBS             1396..1419
                     /label=rbs
     CDS             1420..2139
                     /label=sfGFP
     misc_feature    1396..2139
                     /label=BBa_K515005
ORIGIN
        1 atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg
       61 atggccatga tgtcaagtca gaccacacaa tcttcgaaat tctcgctgtt cctcaagcgc
      121 gggcttctcc ttgcggcagc acccttgctg gcaatgagcg catcctcggc tctcgctgcg
      181 acccaggtaa ccggcttcgg ctcgaacccc ggcaatcttc tgatgtacaa gcatgtgccg
      241 tccagcatgc cggccaatgc gccgctggtc atcgccatgc atggttgcac ccagagcgcc
      301 tctgcctatg aagccaccgg ctggacccag ctcgccaaca cctacaagtt ctatgtggtg
      361 tatccggaac agcaaagcag caataatcag aacaagtgct tcaactggtt cgagcccggc
      421 gacatcgcgc gcggccaggg cgaagccctg tcgatcaagc aaatggtcga caagatgaag
      481 gcagaccact cgatcgatac gaatcgtgtg tatgtcacag gcctgtcggc gggcggttac
      541 atggtcaacg tcatgttggc tacttatccc gacgtctttg ccggcggcgc gcctttttcg
      601 ggcggtccgt acaactgcgc gacttcgatg accaatgcgt tcacctgcat gagcccgggc
      661 gtggataaaa ctccggctgc ctggggcgac ctggcgcgcg gcggctacag cggctatacc
      721 ggccgtaagc cgatcgtatc gatctggcac ggcgatgctg actataccgt caagcaatcc
      781 aatcaggtcg aagaagtgga gcagtggacc aactaccatg gcatcgacca aaccgcagac
      841 gtaagcgaca cggtggccgg cttcccgcac aaggtgtaca aggatgccag cggtaatgcg
      901 ctcgtggaaa cctataccat caccggcatg ggtcatggca ctccggttga tccgggcacc
      961 ggctccctgc aatgcggcac cgctggcgcc tatatcctcg atgtgaatat ctgctcgagc
     1021 tattacgttg ccaagttctg gggattgatc ggcggtagcg gcaccaccac gaccacgtcg
     1081 gctggcacca ctacgactac gtcggcagga accaccacga ctaaggcttc aaccacgacc
     1141 accaaggttt ccactacaac tactgcatcc accacgacga caactgcggg cgcttgctac
     1201 aactcgagca actacgcgca cgtgacggct ggtcgtgctc atgacaccgg tggctatgca
     1261 tataccaacg gttcgaacca gaagatgggg ctgaacaaca ccttctacac cagcaagctg
     1321 cgtaagaccg gtaccaatta ctacgtcatc gacactactt gcccggacta caaggatgac
     1381 gacgacaagt aataaaaatc aaattaagga ggtaagataa tgcgtaaagg cgaagaactg
     1441 ttcacgggcg tagttccgat tctggtcgag ctggacggcg atgtgaacgg tcataagttt
     1501 agcgttcgcg gtgaaggtga gggcgacgcg accaacggca aactgaccct gaagttcatc
     1561 tgcaccaccg gtaaactgcc ggtgccttgg ccgaccttgg tgacgacgtt gacgtatggc
     1621 gtgcagtgtt ttgcgcgtta tccggaccac atgaaacaac acgatttctt caaatctgcg
     1681 atgccggagg gttacgtcca ggagcgtacc atttccttca aggatgatgg ctactacaaa
     1741 actcgcgcag aggttaagtt tgaaggtgac acgctggtca atcgtatcga attgaagggt
     1801 atcgacttta aagaggatgg taacattctg ggccataaac tggagtataa cttcaacagc
     1861 cataatgttt acattacggc agacaagcaa aagaacggca tcaaggccaa tttcaagatt
     1921 cgccacaatg ttgaggacgg tagcgtccaa ctggccgacc attaccagca gaacacccca
     1981 attggtgacg gtccggtttt gctgccggat aatcactatc tgagcaccca aagcgtgctg
     2041 agcaaagatc cgaacgaaaa acgtgatcac atggtcctgc tggaatttgt gaccgctgcg
     2101 ggcatcaccc acggtatgga cgagctgtat aagtaatga
//