LOCUS       BBa_S05241              2308 bp    DNA     linear       22-Dec-2024
DEFINITION  I20260:S05033
ACCESSION   BBa_S05241
VERSION     BBa_S05241.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..782
                     /label=GFP protein
     CDS             63..782
                     /label=BBa_E0040
     stem_loop       802..845
                     /label=stem_loop
     misc_feature    791..870
                     /label=BBa_B0010
     terminator      791..870
                     /label=BBa_B0010
     stem_loop       886..905
                     /label=T7 TE
     misc_feature    912..912
                     /label=stop
     polyA_site      906..919
                     /label=polya
     misc_feature    879..919
                     /label=BBa_B0012
     terminator      879..919
                     /label=BBa_B0012
     misc_feature    1..919
                     /label=BBa_I20260
     CDS             1076..1894
                     /label=kanamycin resistance
     misc_feature    928..1894
                     /label=BBa_P1003
     misc_feature    1929..2308
                     /label=R6K
     misc_feature    1903..2308
                     /label=BBa_J61001
     misc_feature    928..2308
                     /label=BBa_S05033
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      121 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      181 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      241 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      301 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      361 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      421 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      481 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      541 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      601 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      661 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
      721 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
      781 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      901 gggcctttct gcgtttatat actagagctg atccttcaac tcagcaaaag ttcgatttat
      961 tcaacaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     1021 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     1081 ccatattcaa cgggaaacgt cttgctcccg tccgcgctta aactccaaca tggacgctga
     1141 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1201 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1261 caatgatgtt acagatgaga tggtccgtct caactggctg acggagttta tgcctctccc
     1321 gaccatcaag cattttatcc gtactcctga tgatgcgtgg ttactcacca ccgcgattcc
     1381 tgggaaaaca gccttccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1441 tgcgctggcc gtgttcctgc gccggttaca ttcgattcct gtttgtaatt gtccttttaa
     1501 cagcgatcgt gtatttcgtc ttgctcaggc gcaatcacgc atgaataacg gtttggttga
     1561 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1621 gcacaagctc ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     1681 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gggtcggaat
     1741 cgcagaccgt taccaggacc ttgccattct ttggaactgc ctcggtgagt tttctccttc
     1801 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     1861 gtttcatttg atgctcgatg agtttttcta ataatactag agtgattcgc acgggcccat
     1921 ggctaattcc catgtcagcc gttaagtgtt cctgtgtcac tcaaaattgc tttgagaggc
     1981 tctaagggct tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa
     2041 aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta
     2101 tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac
     2161 atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg
     2221 ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt
     2281 agtacgtact atcaacaggt tgaactgc
//