BBa_M45710 1 BBa_M45710 AvrA protein coding sequence 2015-04-15T11:00:00Z 2015-05-08T01:14:09Z http://www.uniprot.org/uniprot/E8XKZ3 This protein has already been sequenced. AvrA is a protein excreted by Salmonella typhimurium (strain 4/74). This protein plays an anti-inflammatory role. false false _1855_ 0 23855 9 Not in stock false The protein does not contain any illegal restriction enzyme sites. No design information is offered at this time, as the submitter of this part did not do any physical work with the protein in a lab. false Nicholas D. Lauritzen BBa_S05264 1 BBa_S05264 R0010:M45710 2015-04-18T11:00:00Z 2015-05-08T01:14:52Z true false _9_ 0 23452 9 Discontinued false false Michelle Bonebrake component2431142 1 BBa_R0010 component2431149 1 BBa_M45710 annotation2431142 1 BBa_R0010 range2431142 1 1 200 annotation2431149 1 BBa_M45710 range2431149 1 207 1109 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961225 1 -10 range1961225 1 161 166 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961223 1 CAP binding site range1961223 1 89 126 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_M45710_sequence 1 atgattttcagcgttcaagaattgagttgcgggggcaaaagcatgttaagtccgacgacccgaaacatgggagctagtttgagccctcaaccggatgtctccggtgagctgaatactgaggctttaacttgtattgtcgaaagattggaatccgagataatcgatggatcatggattcatatcagttatgaagaaaccgatctcgagatgatgcctttcctagtagcgcaggctaacaagaaatatcccgagcttaacttaaaatttgtcatgagcgtacatgaactggtgtcttctatcaaagaaacaaggatggaaggagttgaaagcgcccgctttttggtcaacatgggatcctcgggtattcatatttcggtggtggatttccgggttatggacggcaaaacctccgtcattttgttcgaacctgccgcttgttcagcgtttggtcccgcactggcccttcggacgaaggcggcacttgaacgtgagcagctgcccgattgttattttgcaatggtggaactggatatccaacggtcttcaagtgaatgcggaatcttcagtctggccttggccaaaaaattacagctggaatttatgaatttagttaaaattcacgaagataatatttgtgagcgcctgtgtggggaagagccatttttaccaagcgataaagcagatcggtatctgccggtgtctttttacaaacatacacagggtgcgcagcgtctgaatgagtacgtcgaagctaatccagcggcgggtagttcaattgttaacaagaaaaatgaaacgttatacgaacgtttcgataacaatgccgttatgcttaacgataaaaagctgtcgataagtgcgcataaaaagcgcattgcagaatataaatcactgctgaaacct BBa_S05264_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatgattttcagcgttcaagaattgagttgcgggggcaaaagcatgttaagtccgacgacccgaaacatgggagctagtttgagccctcaaccggatgtctccggtgagctgaatactgaggctttaacttgtattgtcgaaagattggaatccgagataatcgatggatcatggattcatatcagttatgaagaaaccgatctcgagatgatgcctttcctagtagcgcaggctaacaagaaatatcccgagcttaacttaaaatttgtcatgagcgtacatgaactggtgtcttctatcaaagaaacaaggatggaaggagttgaaagcgcccgctttttggtcaacatgggatcctcgggtattcatatttcggtggtggatttccgggttatggacggcaaaacctccgtcattttgttcgaacctgccgcttgttcagcgtttggtcccgcactggcccttcggacgaaggcggcacttgaacgtgagcagctgcccgattgttattttgcaatggtggaactggatatccaacggtcttcaagtgaatgcggaatcttcagtctggccttggccaaaaaattacagctggaatttatgaatttagttaaaattcacgaagataatatttgtgagcgcctgtgtggggaagagccatttttaccaagcgataaagcagatcggtatctgccggtgtctttttacaaacatacacagggtgcgcagcgtctgaatgagtacgtcgaagctaatccagcggcgggtagttcaattgttaacaagaaaaatgaaacgttatacgaacgtttcgataacaatgccgttatgcttaacgataaaaagctgtcgataagtgcgcataaaaagcgcattgcagaatataaatcactgctgaaacct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z